匀浆

After homogenate and centrifugation, the fat existing in liver was removed by diethyl ether. Then, the pH of the remaining aqueous layer was adjusted to 11.6 and clenbutamol was extracted using diethyl ether. The mobile phase component A was 50mmol/L phosphoric acid – 30mmol/L triethylamine and the pH was adjusted to 4.0 with 2mol/L sodium hydroxide.

此外，又将含有盐酸克伦特罗的猪肝用匀浆机匀浆，用1mol/L盐酸溶出其中的盐酸克伦特罗，再用乙醚去除盐酸溶出液中的脂肪后，用2mol/L氢氧化钠调节溶出液pH为11.6，用乙醚萃取其中的克伦特罗。

Then the intracellular calcium concentration of neurons was measured by laser confocal scanning microscope , represented by the mean intracellular fluorescent intensity. On the ice, the cerebral cortical tissue at the infarction margin of the section after optic chiasm was rapidly dissociated about 100mg, then made into brain homogenate in low temperature. The high performance liquid chromatography was used to determine the Glu, Asp and Gly contents in homogenized brain tissue.

视交叉平面用2％TTC染色，用图像处理软件计算出脑梗死面积占该片脑组织面积的百分比；取视交叉前平面梗死边缘区皮层组织约100mg，用酶消化法制作神经细胞悬液，用激光扫描共聚焦显微镜检测皮层神经元细胞内游离钙离子浓渡，以细胞平均的荧光强度代表细胞内钙浓度；冰上迅速分离视交叉后梗死边缘区皮层组织约100mg，于低温下制作脑组织匀浆，用高效液相色谱法测定皮层匀浆中谷氨酸、天门冬氨酸、甘氨酸的含量。

Content of result grackle extraction can reduce liver to injure the serum ALT of small rat apparently, AST and content of MDA of oar of liver divide evenly, XOD of oar of liver divide evenly is active, raise GSH active, liver organizes pathology to change to be reduced apparently.

结果白头翁提取物能明显降低肝损伤小鼠的血清ALT，AST和肝匀浆MDA含量，肝匀浆XOD活性，提高GSH活性，肝组织病理变化明显减轻。

To investigate the method of RNA isolation from human embryonic tissues and the factors influencing the quality of RNA, the RNA from human embryonic tissues obtained with drug-induced labor or non-drug induced labor were isolated by using grind with liquid nitrogen or homogenizer without liquid nitrogen.

为探讨人类胚胎组织完整RNA的分离方法及其影响因素，采用液氮研磨及匀浆器两种制备组织匀浆方法，对不同引产方式的人类胚胎组织进行总RNA分离，并对RNA质量进行鉴定。

Aspirin can delay the progression of lens opacification. Its function is apparent in the earlier stages.

阿司匹林组晶状体匀浆的GSH量、GSH-PX活性及羟自由基抑制能力均高于萘组，而晶状体匀浆的MDA量低于萘组。

The six stages involved euthermic state (25℃), prehibernation (10-12℃), after hibernating for a week and a month (2-4℃), and arousing from hibernation for a week and two weeks (10-12℃). From euthermic state to long-term hibernation, lipid peroxidation was showed to increase in brain except the stage of prehibernation.

分别测试了上述各阶段荒漠沙蜥1％脑组织匀浆、0.5％心脏组织匀浆及1％肝脏组织匀浆中：丙二醛及过氧化氢(H_2O_2)的含量，超氧化物岐化酶、过氧化氢酶、谷胱甘肽过氧化物酶的活性，及总抗氧化能力的水平。

RESULTS: Compared with SG, the levels of Cr and BUN and that of MDA in the nephric tissue were significantly higher than those in OG and TM+OG. The levels of these indices in TM+OG were lower than that in OG. The level of GSH in OG and TM+OG was lower than that in SG, but higher in TM+OG than in OG.

结果：腹主动脉缩窄组及替米沙坦+腹主动脉缩窄组血清Cr， BUN含量及肾组织匀浆MDA水平均较假手术组增高，但TM＋OG低于OG；肾组织匀浆GSH水平OG及TM＋OG低于SG，但TM＋OG高于OG。

The method of pH controlled homogenate extraction of carnosic acid in Rosmarinus officinalis was studied, and the optimum technology parameters were found as following: Homogenate once for 4 min with 80% ethanol (adjusted pH=3.0 with HCl) at a ratio of solid to liquid 1:12.

采用pH控制匀浆法从迷迭香枝叶中提取鼠尾草酸，研究了提取过程中不同影响因子对提取效果的影响。确定的匀浆提取优化条件为：以盐酸调节pH=3.0的80%乙醇为提取溶剂，料液比1：12，匀浆4min，匀浆提取1次。

Analysis the changes of each groups mRNA expression of ICAM-1,VCAM-1 and eNOS .Naphthylamide Heavy nitrogen spectrophotography was adopted to detect thecontent of nitric oxide in the blood serum. To try to find out the mechanism of QDTMT prevent and cure function on AS.

采用蔡胺重氮分光光度法测定血清中NO含量，采用半定量RT一PCR的方法分别检测各组动物动脉壁匀浆及单个核细胞中ICAM一l、VCAM一1 mRNA的表达，以及动脉壁匀浆中eNOSmRNA表达，并分析造模及各药物组上述指标mRNA表达的变化；进一步探讨QDTMT防治As作用的机制。

Then killed the animals and extracted hippocampus.The serum of TChE,SOD CAT and MDA were detected.

然后将各组剩余小鼠断髓处死，冰台上迅速取出脑组织，在预冷的生理盐水中漂洗，除去血液，分离出两侧的海马，投入4℃冰浴的玻璃匀浆器中，按1∶9于生理盐水中制成10%脑组织匀浆，在4℃下4000r/min离心10min，取上清液以检测小鼠海马组织中乙酰胆碱酯酶、超氧化物歧化酶、过氧化氢酶的活力和丙二醛的含量。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。