匀和

Methods Established the Wistar rat models of EAE by footpad injection of guinea pig spinal cord homogenates and CFA. Animals were executed 6 days after disease onset, and optic nerves, brains and spinal cords were removed for light and electron microscope pathological analysis.

足垫皮下注射豚鼠脊髓匀浆和完全弗氏佐剂混合物制作Wistar大鼠EAE模型，于发病后第6 d将大鼠处死，取视神经、脑和脊髓，行HE和LFB染色，光镜和电镜下观察其病理改变。

Results: The result is that PCNA positive appeared 6 hours after transplantation, increase unit time pass by, and reach peat at 1 to 2 week. After 2 weeks, PCNA positive of SMC in the tunica media begin to decline and recover to the level 6 hours after the operation at the 8 weeks.

本实验的第三部分，我们将于液氮固定的移植静脉段经组织匀浆后用于研究ECE和ET-1的表达情况，我们分别从mRNA水平和蛋白质水平，采用RT-PCR和免疫组织化学方法，对ECE和ET-1在移植静脉的表达情况进行分析。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

When the night got down quietly, the glory which seemed to be the jade fell into the entire earth and space looking like the Milky Way waterfall. It suddenly shone the clear bright. The silent magnolia looking like a literary-style girl by now on, calmly bathed in the fantastic night. In another hand, it was decorated gracefully and naturally by the flowers which were standing upright the branches showing off some extent of grazioso pretty. Looking the suitable ray of light and paying the full attention to the top of the tree gave me more interest.

当夜沉寂下来，一丝风也没有，玉一般的光辉似银河瀑布匀匀地倾洒向整个大地和空间，映照出一种清晰的明亮，沉默不语的花树这时就如一个文气的姑娘，静静地沐浴在夜的飘渺之中，而那挺立枝头非常大方的花又把她装点得婀娜多姿，显出一派幽幽的靓丽。

When the night got down quietly, the glory which seemed to be thejade fell into the entire earth and space looking like the MilkyWay waterfall. It suddenly shone the clear bright. The silentmagnolia looking like a literary-style girl by now on, calmlybathed in the fantastic night. In another hand, it was decoratedgracefully and naturally by the flowers which were standing uprightthe branches showing off some extent of grazioso pretty. Lookingthe suitable ray of light and paying the full attention to the topof the tree gave me more interest.

当夜沉寂下来，一丝风也没有，玉一般的光辉似银河瀑布匀匀地倾洒向整个大地和空间，映照出一种清晰的明亮，沉默不语的花树这时就如一个文气的姑娘，静静地沐浴在夜的飘渺之中，而那挺立枝头非常大方的花又把她装点得婀娜多姿，显出一派幽幽的靓丽。

Cosmic spherules were discovered among artificial heavy mineral grains in Changzhogou formation, Taoyuan, Lunxian;Changzhogou, Jixian of Hebei and the Ming Tombs , District of Beijing.

河北滦县、蓟县、北京十三陵三个地方的常州沟组宇宙尘是在人工重砂的重矿物中发现的，产在古老沉积岩地层中的砾岩和砂岩里，大部分是铁质宇宙尘，形态各异，表面构造多种多样，内部构造各有不同，具Fe—Ni金属，核与壳的化学元素分布不匀匀，化学成分与深海宇宙尘相似。

Add into the penne and other ingredients.

将调味料拌匀，加入长通粉和其他材料再拌匀，放入雪柜冷冻便可供食。

A deviation to enable business card printing and membership card making more neatly away material; business card printing and membership card Studio Center-drum for double-deck, using thermostatical hollow water circulation system structure, plated nickel plating avoids corrosion, Nano-imprint lithography drum machine, safe and reliable braking equipment; spam satellite business card printing and membership card, four business card printing and membership card production unit in the Centre of coining drum distribution around the business card printing and membership card making support adoption of the integral structure, intensity that cannot be easily; Center for the imprint drum version, roller roller, using separate servo motors between the gearless, Business card printing and membership card making length is not supported by gear pitch in roll-wide choice; business card printing and membership card making pressure, roller and roller pressure singlein servo motor and with memory with two types of regulation, accurate and rapid adjustment; closed ceramic Roller blade device ink to ensure that the business card printing and membership card making ink stability, blade-like device for pneumatic compression; taojian the plate cylinder, taojian-reseaus and roller, the portal structure of push-pull roller and taojian beckenbachannular roller to replace a simple and quick; roller servomotor-driven business card printing and membership card production and business card printing and membership card making machine be a certain speed, stopping the slow down to prevent the ink roller network hang dry knot, splash-proof ink installed equipment downtime or roller pressure, from the printing plate out of arrive﹖, stop to ink, the plate cylinder the full delivery from the pressure to keep the printing plate clean; stainless steel-monlchamus tuhung-glass to prevent ink prints get dirty; dryer direct to the wind, air flow loss small high efficiency, reduces noise scene, new oven heat energy to secondary structures, control temperature control system using intelligentized; traction roll at the same time acts as both a cooling rolls, new structure increases cooling effect.

纠偏，使制卡和会员卡制作走料更加整齐；制卡和会员卡制作部中心大滚筒为双层空心式，采用恒温水循环系统结构，表面镀镍处理可避免锈蚀，中心压印滚筒采用机械制动装置，安全可靠；卫星式制卡和会员卡制作方式，4个制卡和会员卡制作单元分布在中心压印滚筒周围，制卡和会员卡制作支架采用整体式结构，强度好不易变形；中心压印滚筒、版辊筒、网纹辊之间采用独立伺服电机无齿轮传动，制卡和会员卡制作长度不受齿轮节距限制，在版辊范围内任意选择；制卡和会员卡制作压力、网纹辊与版辊之间的压力分别用伺服电机调整，并具有记忆功能，调整有两种调节方式，精确调整与快速调节；陶瓷网纹辊封闭刮刀装置供墨，可确保制卡和会员卡制作过程中油墨稳定饱满，刮刀装置为气动加压；套筒式印版滚筒，套筒式网纹辊筒，门式推拉式结构使套筒版辊与套筒网纹辊更换简单快速；网纹辊由伺服电机驱动，制卡和会员卡制作时与制卡和会员卡制作机保持相对一定的速度，停机时则放慢速度匀墨，防止网纹辊网坑枯结，两边安装防油墨泼溅装置，在停机或离压时，网纹辊立即从印版脱离开来，停止给墨，印版滚筒把墨完全传递后离压，保持印版清洁；不锈钢接墨斗，防止油墨滴漏蹭脏印品；干燥箱采用直接进风型式，风量损失小效率高，减小了现场噪音，新型烘箱结构热能可二次利用，控制采用智能化恒温系统；牵引辊可同时兼作冷却辊使用，新型结构增大了冷却效果。

Surmise cake is the most critical aspect of steamed puddings immediately opened the lid, still hot steam out into the pot in the pudding as soon as some cold water back and forth through the hot-dip folded with the fist and the hides to make them uniform in a uniform circular block.

揣糕是最为关键的环节，糕蒸熟要立即揭开锅盖，趁热汽将糕取出放入瓷盆中，马上借热沾些凉水来回用拳折和揣，使其变成匀匀的一圆块。

Gradually add in egg, coconut shreds and evaporated milk.

鸡蛋打散后分次加入拌匀后倒入椰丝和淡奶拌匀。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。