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objective to compare the anti-aging effects of lycium barbarum polysaccharides and tanshinone on the skin of d-galactose-induced mice aging model.methods 60 female mice were randomly divided into 4 groups (n=15):mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of d-galactose[1000 mg/(kg1·d)] for 42 d to establish subacute aging model.the experimental groups included agingmodel group,lycium barbarum polysaccharides [200 mg/group,tanshinone [1 500 mg/] group.forty two days later,the dorsal skin samples were collected to determine sod activities、mda and hydroxyproline contents.

60只雌性昆明种小鼠,随机分为4组(n=15):正常对照组皮下注射生理盐水;其余3组每日颈背部皮下注射 d-半乳糖[1000 mg/]造成小鼠衰老模型,同时各组分别灌胃生理盐水、枸杞多糖[200 mg/]、丹参酮[1 500 mg/]。42 d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

A polyclonal antibody against vitellin was prepared in male rabbits and the specific antibody against vitellin was made through absorbtion by male worm homogenate. After purification, HRP was used to mark antibody, then ELISA was set up to detect vitellin content in the Chrysopa septempunctata fat-body, hemolymph and ovary.

提取了大草蛉的卵黄蛋白,免疫家兔,并将所得抗血清经雄虫匀浆液吸收,获得抗卵黄蛋白的特异抗体,经纯化后,用辣根过氧化物酶标记抗体,建立了ELISA法测定大草蛉脂肪体、血淋巴和卵巢中卵黄蛋白含量的方法。

After varied survival periods, the model mice were sacrificed to get the specimens of SN fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry to label the DA neurons and Calbindin D28k-containing neurons with the antibodies against tyrosine hydroxylase and CR respectively. The labeled cells were counted under microscope and analyzed statistically.

模型动物存活1周后,一部分取其中脑黑质节段,固定、包埋做连续冠状石蜡切片,以免疫组织化学染色方法,显示各组酪氨酸经化酶和钙结合蛋白表达阳性细胞,光镜观察并细胞计数,统计学分析;另一部分取其中脑黑质组织匀浆,行TH、CB的Westem blot免疫印迹方法检测,用LabWorks软件分析处理。

Methods: A sepsis model of mice made with cecum deligation and perforation was established,and a radioimmunoassay for FABP and 96-well spectrophotometry assays for myeloperoxidase and superoxide dismutasewhich were related with clearance of free radicals,were used to detect their levels in lung and intestine ho...

建立小鼠盲肠结扎致脓毒症模型,采用放射免疫分析法和96孔分光光度分析法分别检测肺肠组织匀浆液中FABP及髓过氧化物酶、超氧化物歧化酶等与自由基清除相关的酶的水平、同时以HE染色方法观察肺肠组织病理组织学改变。

A virus strain with high hemagglutinin titer was isolated out from embryonated egg and MDCK cells after they were inoculated with the lung tissues of the died marmosets.

在排除细菌感染的基础上,通过死亡狨猴肺组织匀浆接种鸡胚和MDCK细胞的分离培养,分离出一株具有很高血凝效价的病毒株。

A virus strain with high hemagglutinin titer was isolated out from embryonated eggs and MDCK cells after inoculating with the lung tissues suspension of the dead marmosets.

在排除细菌感染的基础上,通过死亡狨猴肺组织匀浆接种鸡胚和MDCK细胞的分离培养,分离出一株具有高血凝效价的病毒株。

Product fault ink UN-uniform UN-uniform ink products were:(1) water and ink, ink and wash painting the rubber roll version of wear, spindle bearing wear, cots epiderm aging decline, ink, etc;(2) series water roll of layer debranning, cylindricity wear, a thin layer of shoulderpieces for intergradle monlchamus, a product of long a portion of the Ribbon, resulting in a roller 跞 movement oscillating anisotropism;(3) support by Edition of water, rollers, the cylinder, swayig stand, wear, resulting in version tiaodong Cot by cause pressure by version is unstable, produce ink imbalance, ink, ink plate, staves, relying on the old version (students'overloaded) version of COTS and pressure;(4) the water roll chemiluminscent set of aging or poor quality water decline caused by transmission;(5) does not have a regular check of the drum-and-ink roller and version by version-and-ink roller and pressures;(6) if the drum gear wear, and gear spacing is the same as the regular ink "" gear.

二、产物墨色不平均障碍产物墨色不平均的来因有:(1)传水、传墨、匀墨和靠版水墨各橡胶辊的圆柱度磨损超不差,拆轴承的轴脚磨损,胶辊不内脏嫩化,传墨本领回升等;(2)串水辊度层脱皮,圆柱度磨损超不差,暗地有薄积墨层,两脚长出局部有积墨带,变不败滚子跞不静串墨不不平衡;(3)撑持靠版水、墨辊的锁脚,摆不静支架,磨损惨重,变不败胶辊靠版时跳不静致使靠版不抬辛不安定,爆发水墨不不平衡,呈现墨色不不平衡、干版、水墨杠、嫩版等本体;(4)水辊绒套嫩化或质度不差变不败传水本领回升;(5)操纵按期校核各水墨辊及版滚筒与靠版水墨辊的不抬辛;(6)若滚筒传不静齿轮磨损超不差,呈现与齿轮间距不异的有次序的&齿轮墨杠&。不一、套印障碍 1。

The idea here is to introduce very subtle surface texture changes that represent the slight uneveness brought on by MBT vents, bouys, floodable grating covers and hatches.

这里的想法是引入非常微妙的表面纹理的变化,代表轻微不匀带来的主战坦克是由喷口, bouys , floodable光栅盖和舱口。

The analysis of existing insect diversity showed that there are species of the common insects, except Coleoptera and Hemiptera, there are no more than 20 species among the other 9 orders of the insect species, and the index of species Shannon-wiener is low.

昆虫物种多样性分析显示:Shannon-Wiener指数较低,该区常见昆虫种类较少,除鞘翅目和半翅目昆虫外,其它9个目的昆虫种类匀不超过20个种类。

Objective To study the leucocytes infection statistics of the tiny organism eperthrozoon and analysis on the people who just reached the highland and who inhabited the highland for half a year at least.Methods Take one portion of anticoagulation that contains abundant leucocytes,two portions of distilled water or1%HCl2liquores for dilution,and mix them up to hemolyze for ten minutes.Take15ul of the mixture to make a silce,and then observe by oil microscope.

目的 研究从内地不同地区急进高原人群和其在高原环境中居住半年后白细胞内感染附红细胞体的对比分析方法取富含白细胞的抗凝血液1份,加蒸馏水或1%HCl2份稀释混匀,使其溶血10min,取15μl制作压片然后用油镜观察。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。