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After varied survival periods, the model mice were sacrificed to get the specimens of SN fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry to label the DA neurons and Calbindin D28k-containing neurons with the antibodies against tyrosine hydroxylase and CR respectively. The labeled cells were counted under microscope and analyzed statistically.

模型动物存活1周后,一部分取其中脑黑质节段,固定、包埋做连续冠状石蜡切片,以免疫组织化学染色方法,显示各组酪氨酸经化酶和钙结合蛋白表达阳性细胞,光镜观察并细胞计数,统计学分析;另一部分取其中脑黑质组织匀浆,行TH、CB的Westem blot免疫印迹方法检测,用LabWorks软件分析处理。

The results indicated that the water or 50% ethanol extracts from peonies petals could reduce light absorption of DPPH and OH initiated hydroxylation of salicylate, and inhibit O2(superscript -) mediated light chemical reduction of nitroblue tetrazolium effectively, and lipid peroxidation of polyunsaturated fatty from yolk lipoprotein, in which DPPH scavenging activities of 6 peonies water extracts were hinger than that of 200 μg/mL BHT, but lower than 80 μg/mL Vc. In 4 kinds of detecting systems of antioxidation, the peroxidation activity of deep colored flowers of peonies was higher than that of light colored flowers of peonies.

牡丹花瓣提取液能明显抑制卵黄组织匀浆的脂质过氧化作用,降低DPPH的吸光值、抑制O2介导的NBT光化学还原及OH作用下的水杨酸羟基化作用,其中牡丹花瓣提取液清除DPPH自由基的活性高于200μg/mL BHT,但低于80μg/mL Vc.6个品种的牡丹花瓣提取液,在4种测定系统中均具有一定程度的抗氧化能力,且水提取液的抗氧化活性略高于体积分数50%乙醇提取液,深色花瓣提取液对不同自由基的清除能力较强。

Intercalation of metal complexes into the interlamellar space of clay is an excellent method for homogeneous catalyst heterogenization .

粘土的可膨胀的二维空间有助于将催化活性的金属络合物用阳离子交换法和配位体交换法插入到粘土层间,因而易于制得粘土层间金属络合物催化剂,这是匀相催化剂多相化的一种好方法。

Preparation and Properties of A Chloroperoxidase-like Catalytic Antibody Iodination of free tyrosine and tyrosine in protein is m...

游离酪氨酸和蛋白质中酪氨酸的碘化是由甲状腺匀浆液和亚细胞部分中存在的酶催化的。

Lavement of pulmonary alveolus of right lung bronchus, undertake left lung of; of cellular classification, computation organizes divide evenly oar to determine group of immunity of collagen content; changes a law to determine sex of STAT1, plaque source grows factor the expression of albumen of 1(PAI-1 of depressor of content of activation of PDGF and fine dissolve zymogen.

右肺支气管肺泡灌洗,进行细胞分类、计数;左肺组织匀浆测定胶原含量;免疫组化法测定STAT1、血小板源性生长因子和纤溶酶原激活物抑制剂1(PAI-1)蛋白的表达。

In the experimental study section, the rats were divided into four groups, including the normal control, the model, the Piracetam, the QPC, and copied to AD model. After the treatment, we observed the effects on the AD rats' ability of leaning and memorizing, detecting the TChE activity in whole blood and the expression levels of the related biochemical indicators of As includeing GFAP、NGF and BDNF of the AD rats' brain hippocampus, then compared the QPC with other groups to reveal the mechanism of action of the QPC treating AD to bright.

在动物实验部分,我们通过塑造AD动物模型,设立正常对照组、模型组、脑复康组和强脾益智胶囊组,观察强脾益智胶囊对AD模型大鼠空间学习记忆能力的影响,检测AD大鼠全血、海马匀浆液中乙酰胆碱酯酶活性和通过免疫组化的方法检测胶质纤维酸性蛋白、神经生长因子、和脑源性神经营养因子的表达情况来反映星形胶质细胞的增值情况,以期揭示强脾益智胶囊对AD的治疗作用机理。

The techniques in the production of elegant type Luzhou-flavor liquor covered the following: blending of liquor of different flavor types and production control of base liquor (including raw materials, saccharifying leaven, production equipments, fermentation techniques, and microorganism etc).

生产浓香淡雅型白酒的技术有:采用不同的香型酒来组合匀兑和通过时原酒的生产控制来解决(包括原料、糖化发酵剂、生产设备、发酵工艺和微生态等方面)。

The leaves soft, fat and elastic, the good; hard and inelastic, not good; The color red-brown and even, the good; uneven, black, carbonized, and putrefied such as mud and leaves not opening, not good quality.

叶质柔软、肥嫩、有弹性的好,叶底硬、无弹性的品质不好;色泽褐红、均匀一致的好,色泽花杂不匀,或发黑、碳化、或腐烂如泥,叶张不开展属品质不好。

Low dose Yiweitang group; 4. Diethylstilbestrol group; 5. Model control group. After 4 weeks intervention, get the left ovarie, a part of hypothalami and pituitary gland from head-off rat, and observe expression of ER, FSHR and GnRH by immune histochemistry method . Then extract RNA from the rihgt ovarie and the rest hypothalami and pituitary gland, so as to measure the expression of GnRHmRNA 、 ERmRNA 、 FSHRmRNA and P 16mRNA by RT-PCR. And the cerebrum were made to homogenate to examine SOD, GSH-PX and MDA.

各组实验动物灌药4周后,断头处死,取部分下丘脑、垂体及左侧卵巢组织固定切片,免疫组化法检测 ER 和 FSHR 表达、 GnRH ;取剩余部分下丘脑、垂体及右侧卵巢组织提取总 RNA ,用于 RT-PCR 检测下丘脑 GnRHmRNA ,下丘脑、垂体的 P 16mRNA 及下丘脑、垂体、卵巢的 ERmRNA 、 FSHRmRNA 基因表达;另外取部分大脑组织制作脑组织匀浆,用于 SOD 和 GSH-PX 酶活力以及 MDA 含量的测定。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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