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Phenanthroline ; spectrophotometry ; red mud ; limestone ; mixedness degree

邻二氮菲;分光光度法;赤泥;石灰石;混

Detection the early stage apoptosis cells by flow cytornetry Incubating SCL-1 cells in 6-well culture medium with 1×10~6/well, after 12~48hculture with the concentration of imiquimod 0μg/mL and 150μg/mL, gathering cellsgrowing along the wall and making monocellular suspension, washing in PBS for 2times, and then add 5μL Annexin V/FITC and 10μL PI solutions, reaction for 15min avoiding light, detection the early stage apoptosis cells by flow cytometry.

流式细胞仪测定细胞早期凋亡 SCL-1细胞以1×10~6个/孔接种于6孔培养板中,使用含0μg/mL、150μg/mL咪喹莫特的培养液培养12~48h,收集贴壁生长的细胞制成单细胞悬液,PBS洗两次,分别加入5μL Annexin V/FITC和10μL浓度20μg/mL的PI溶液,混后室温避光孵育15min,流式细胞仪检测,结果用Cell Quest软件分析。

Overloaded rat models were established by using abdominal aorta constriction method. The levels of creatinine and urea nitrogen in the rats?? serum as well as those of malonaldehyde and glutathion of the rats?? nephric tissue were measured respectively.

采用腹主动脉缩窄法制备压力超负荷大鼠模型,分别测定大鼠血清肌酐和尿素氮含量及大鼠肾组织浆丙二醛、谷胱甘肽含量。

The sol-gel solution was prepared by mixing ethyl orthosilicate, absolute alcohol and 0.1molL^(-1) HCl in a definite proportion, and adding an appropriate amount of silicotungstic acid as active material, strring for 30min and then standing for 24h at room temperature.

溶胶-凝胶溶液系将正硅酸乙酯、无水乙醇及0.1molL^(-1)盐酸按一定比例混合后加适量的硅钨酸作为活性物质,搅拌30min混后在室温放置24h配成。

Take care not to overmix.

即可,不要过度搅拌。

Takecare not to overmix.

即可,不要过度搅拌。

For every 4 overbeaten whites, you can try whipping in about 3/4 of 1 egg white, stir unbeaten white in a bowl, then spoon some into the overwhipped whites and beat again, carefully, for 20 to 30 seconds.

每四个打发过度的蛋白中,可加入一颗新蛋白的3/4,轻轻的用搅拌器拌约20~30秒即可。

The synthesis method of the invention is as follows: first, 5-amino-2-(p-aminophenyl)- benzoxazole and aliphatic diacid are respectively recrystallized; NMP is distilled; solubilizer is dried; second, in an atmosphere of nitrogen, after Py and NMP are mixed evenly and added to a sealed reactor, the solubilizer is added into the reactor, followed by the 5-amino-2-(p-aminophenyl)- benzoxazole, then the mixture in the reactor is stirred; second, in an atmosphere of nitrogen, the mixture in the second step is stirred and heated to 50-150 DEG C, then the aliphatic diacid is added, followed by TPP for condensation; fourth, after methanol is poured into the reactor, the sediment is separated out from the mixture, filtered, washed and dried, so the semi-aromatic polyamide containing oxazole ring is obtained.

本发明的合成方法如下:一、分别重结晶5-氨基-2-苯并唑与脂肪族二酸;蒸馏NMP;干燥增溶剂;二、在氮气气氛下,Py与NMP混后加入密封的反应器中,再加入增溶剂,然后加入5-氨基-2-苯并唑,搅拌;三、在氮气气氛下,将步骤二的混合液搅拌并升温至50℃~150℃后加入脂肪族二酸,再加入TPP后缩聚;四、再倾入甲醇析出沉淀,过滤,清洗沉淀,再干燥;即得到含唑环半芳香聚酰胺。

Mix drained rice with the 300ml coconut milk, 100ml water, pandan leaves and peppercorns. Pour onto the lined steaming tray. Steam for 15 minutes.

把300毫升椰奶、100毫升水、班兰叶、白胡椒粒和沥乾的糯米拌,倒入铺好的焗盘里,蒸15分钟。

objective to search the factors of liver injury via the anit-induced liver injury in rats.methods male sprague-dawley rats,were divided into 4 groups,data were analyzed using a completely randmized factorial anova and linear correlation.results after anit intoxication,the necrosis of hepatic cell and neutrophil infiltration im periportal region of the liver were observed in liver setions.jinshisan can reduce the alt,the tbil.pla2 correlated positively with alt、akp and tbil.conclusion the severity of anit-induced liver injury correlated positively with the pla2.jinshisan can protect against the rat anit-induced liver injury.

目的 拟通过对α-萘异硫氰酸酯诱导大鼠肝损伤发病机理的探讨,对引起肝损伤的因素进行系统的研究。方法选择s-d大鼠,雄性,随机分4组,检测数据以平均值±标准差表示,采用方差分析,相关分析进行统计学处理。结果s-d大鼠经anit染毒后,病理切片显示肝损伤明显,汇管区有大量中性粒细胞浸润。经金石散及地塞米松治疗后,生化指标均有所下降,中药金石散优于地塞米松,血生化指标与pla2活性相关分析显示,呈正相关。结论 anit诱导的肝毒性损伤程度与肝组织浆pla2活力呈正相关,应用抑制剂降低pla2活力,肝损伤程度也减轻,中药金石散具有降低pla2活性,减轻肝毒性损伤。

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