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The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Taking natural anhydrate(anhydrous gypsumⅡ) as major raw material,adding composite activator for modification to adjust setting time,flexural strength and compression strength,then mixed and milled with proper amount of composite water-retention agent,fly ash and air entraining agent to obtain plastering gypsum .

以天然硬石膏为主要原料，掺加复合激发剂进行改性处理，调节凝结时间及抗折、抗压强度，然后辅以适量复合保水剂、粉煤灰掺合料、引气剂等，磨细混匀后即得到抹灰用的粉刷石膏。

Is located in the Qingpu District of Shanghai Huqingping 2599, Panda high-tech industrial park, is set research and development, manufacturing, sales automatic Jiaoyun sewage pumps, Sets, air compressor, antiscale Centrifugal pumps, constant pressure fire pump, no suction pipe network frequency automatic pressurized water supply equipment, pipeline cleaning equipment, frequency conversion kits water supply equipment, control cabinet, pressure complete sets of equipment in a body specialized company.

上海熊猫机械集团有限公司坐落于上海市青浦区沪青平公路2599号熊猫高科技工业园区内，是集研究、开发、制造、销售自动搅匀排污泵、高压清洗机、空压机、防垢离心泵、恒压消防泵、变频无负压管网自动增压给水设备、管道清理设备、变频成套供水设备、控制柜、气压成套设备于一体的专业集团公司。

In APAR simulator system design scheme a new design is used to generate all the sum, azimuth difference and elevation difference channel target echo signal by passing only one channel signal of target through antenna gain multiplication circuit, which simplified the simulator design.

提出了在很短的一帧时间里用匀加速直线运动拟和目标实际运动轨迹的方法，简化了目标回波模拟系统并实现了目标数据的实时模拟。

Aseptically remove a sufficient quantity of solids from the appropriate amount of containers (see Table 2), mix to obtain a composite, equivalent to about 6 g of solids, and transfer to a sterile 500-mL conical flask.

以无菌操作从适当数量的容器中（见表2）取出足够数量的固体，混匀以获得等同于6g固体的混合物，转移至一个无菌的500mL锥形烧瓶。

Objective To study antioxidation ofDi-(3β-hydroxyandrost-5-en-17-one) ester, a new compound which was structure derivated from active substance from Asterias amurensis.

目的 以兔和大鼠心肌及肝组织匀浆为材料，研究一种从多棘海盘车中提取得到的活性物质经结构改造和修饰得到的丁二酸二-（3β-羟基雄甾5-烯-17-酮）酯新化合物抗脂质过氧化作用。

In the cytosol preparations, nimodipine inhibited the iNOS enzymatic activity induced by LPS +〓 in mouse astroglia cells with an 〓 value of 0.036±0.003mM and nNOS activity in mouse neurons with an 〓 value of 0.047±0.003mM, but did not affect the eNOS activity in bovine cerebral endothelial cells.

在体外培养细胞制备的匀浆液，尼莫地平抑制由LPS+〓诱导的星型胶质细胞的iNOS活性，〓为0.036±0.003mM，同时还抑制神经元的nNOS活性，IC50为0.047±0.003mM，但对牛脑微血管内皮细胞的eNOS活性无明显影响。

M and appearance of many long pseudopodia, have a lot of differentsized basophilic cytoplasmic granules. Some of the cells have both large and small cytoplasmic vacuoles, which can not be stained by neutral red.

m，胞质中具有大小不匀的许多嗜碱性颗粒，有些细胞内也含有不为中性红所着色的较大空泡，细胞表面有许多长伪足伸出。

objective the effects of anthocyanin pigment from maize purple plant on resisting lipid peroxidation were investigated.methods the inhibition of anthocanin pigment from maize purple plant was examined in vitro that autcoxudation of lecithin liposome system induced by fe2+.50 mice were randomly divided into 5 groups.vehicle and different dose anthocyanin pigment from maize purple plant were given respectively,and then experimental injury of mice liver was induced to make use of bromobenzene and the content of mda was determined in liver homogenate.results the inhibition rates of anthocyanin pigment from maize purple plant on linoleic acid oxidation was higher that of ascorbic ascorbic acids.the content of mda in homogenate in middle and high dose of pigment were significantly lower than that in low dose and the injured control group.there were no significat differences in the content of mda in homogenate between low dose group and injury control group.conclusion the anthocyanin pigment from maize purple plant have the capability to resist lipid peroxide.

目的 探讨玉米紫色植株色素抗脂质过氧化的作用。方法体外实验测定玉米紫色植株花色苷色素在fe2+引发的卵磷脂脂质体体系中抗氧化活性。体内实验，取50只小鼠随机分成5组，分别给予溶媒和不同剂量的色素，然后采用溴代苯致实验性肝损伤，测定肝匀浆的丙二醛含量。结果在由fe2+引发的卵磷脂脂质体体系中玉米紫色植株色素对脂质过氧化有明显的抑制作用，抑制率随样品的浓度增高而增大，并且明显优于抗坏血酸。在溴代苯致小鼠实验性肝损伤模型实验中，中、高剂量组的丙二醛含量均低于损伤模型组。低剂量组和损伤模型组比较丙二醛含量差异无统计学意义。

The findings of this study demonstrate the presence of enzymes in S. Japonicum, which are able to catabolize the heme.

成虫匀浆抽提物体外降解血红素的反应对pH值有依赖性，HO和BR反应的最佳pH环境均为pH 8.7。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。