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Then rAAV-DJ-1 was transfected into HEK-293 cells for packaging. AAV vectors encoding human DJ-1 protein were stereotactically injected into the rat SN 4 weeks prior to rotenone lesion in SN. Tyrosine hydroxylase protein was determined using immunohistochemical staining. Ethovison software allowed measurement of movement distance in 30 minutes.

并转染HEK-293细胞,进行腺相关假病毒颗粒包装,所得病毒颗粒注射到大鼠脑内感染黑质神经细胞;4周后,注射鱼藤酮于大鼠黑质相同脑区;通过免疫组织化学方法鉴定TH蛋白表达情况,采用动物行为轨迹分析软件计算大鼠30 min内运动距离。

Neural stem cells exist in numerous regions of the central nevous system and have capacity to differentiate into neurons. Noggin promotes adult neurogenesis of subventricular zone and subgranular zone by blocking endogenous bone morphological protein signaling under physiologic state.

神经干细胞存在于中枢神经系统的广泛区域,且这些细胞具有分化为神经元的潜能。noggin通过拮抗内源性骨形态蛋白(Bone Morphological Protein, BMPs)的作用促进生理状态下成年动物室下区和海马齿状回成体神经元发生。

To result in 20 percent left tibia middle and inferior diaphyseal defect of every goat and fixed with half-ring sulcated external fixator.

在体支架材料内BrdU标记自体MSCs增殖的大动物示踪研究:培养山羊MSCs,传至第三代,细胞同步化后加入BrdU培养。

"Sleep after learning helps solidify memory," says Susumu Tonegawa, PhD, a Nobel Prize-winning professor of biology and neuroscience at the Picower Institute for Learning and Memory at MIT. According to animal studies, when you perform a task, the brain cells fire in a certain sequence.

诺贝尔奖得主麻省理工大学学习和记忆Picower学院的生物学和神经科学教授Susumu Tonegawa博士说:&睡眠能够巩固记忆&根据对动物的研究,当你在进行一项任务时,大脑细胞以一个特点的顺序运行。

Dissociate cortex cells from early postnatal wistar rats (postnatal hour 24) with DMEM were supplemented by horse serum and N〓 medium, and non-neuronal cell division was halted by cytosine arabinoside. As the result, enough neurons with extensive and synaptically active network were produced.

实验各组分别在造模后3、7、15天取材,观察动物一般情况、脑系数、脑组织含水量、脑组织HE、Nissl's、Mallory染色、投射电镜形态学变化以及TUNEL染色显示凋亡细胞等指标变化,并采用计算机图像分析系统对相关形态学指标进行定量分析。

After successful setting up the mold, the main organs' tectological changes of big rats can be observed through a light mirror.

动物模型的制作均获得成功,光镜下观察心脏、肺脏、肝脏、肾脏、脑组织细胞形态均有明显的异常改变。

Objective To establish an animal model of thyrotoxic myopathy, and to observe the morphological changes of its skeletal muscle cells after treatment with Xiaoyingqiangjitang (XYQJT,a traditional Chinese medicine).

目的建立甲状腺功能亢进性肌病的动物模型,观察消瘿强肌汤治疗后大鼠骨骼肌细胞的形态学变化。

As a member of POU transcription factor family, Oct-4 plays an important role in mammalian early embryonic development and takes part in maintenance of cellular totipotence and undifferentiated state.

Oct-4属POU家族蛋白,是一类在动物早期胚胎发育过程中起重要作用的转录因子,参与维持细胞的全能性及未分化状态。

And 37. 1%, respectively. The results suggest that 18α-GA down-regulated CYP expression at the transcriptive levels.

表明GL在转录水平下调大鼠肝细胞CYP1A1,CYP2E1和3A1表达,以对CYP2E1的抑制作用最强,对CYP3A的抑制作用最弱,对CYP1A1的抑制作用居中,与整体动物的酶学实验结果基本一致。

T739 mice were inoculated with LA795 cells. Angiostatin was given to part of inoculated and uninoculated mice by intraperitoneal injection fourteen days after the inoculation. The treatment lasted 20 days and the size of tumor, survival period, behavior of the mice and pathology of lungs, livers and kidneys were observed.

用亲和层析方法得到的血浆纤维溶酶原经胰蛋白酶消化、层析柱亲和层析、透析,得到纯化的血管抑素;以LA795细胞接种于T739小鼠;接种后第15日起腹腔内注射血管抑素共20 d,观察动物行为,测量荷瘤鼠肿块直径,记录生存期,另设未治疗对照组及观察血管抑素副作用对照组,行肺、肝、肾脏病理学检查。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。