动物细胞

Tetraploid embryo can be used as a power tool on life science such as on the research of mechanism in the inchoate embryo development which regulatory the cell volume, mumber and cleavage rate. Tetraploid embryo can also be used to change the ratio of genome of parents on the progeny; More importantly we can obtain completely ES cell-derived mice through tetraploid embryos complementation and the ES mice is significance in analysis of gene function and built disease mouse modle.

在生命科学研究中，四倍体胚胎可以作为研究机体发育的有力工具，例如，用它来研究早期胎儿发育过程中调节细胞体积、数目以及分裂速率的机制，也可以用它来改变双亲基因组在子代中的分配比例；更为重要的用途是通过四倍体补偿作用获得完全由ES细胞发育而来的个体，ES小鼠的获得对基因功能的分析、疾病动物模型的建立有十分重要的意义。

Allen was a researcher at the Department of Neurobiology, The Babraham Institute, Cambridge, UK. Dr. Allen's research skills and techniques include DNA/RNA manipulation and purification/isolation, electrophoreses, PCR, cloning, all blottings, library screening, genetic mapping, design and construction of transgenes, artificial chromosome engineering, design and construction of vectors for gene targeting in ES cells, cell culture, histological techniques, mouse behavioural analysis, small animal surgery, and bioinformatic tools such as sequence databases and analysis tools.

从1996年到 2004年，Dr。 Allen 一直在英国剑桥Babraham 研究所神经生物学部门从事研究工作，包括DNA/RNA操纵、提纯/分离、电泳、PCR、克隆、印迹工作、文库筛选、遗传图绘制、转基因设计与构建、人工染色体工程、用于ES细胞基因打靶的载体设计与构建、细胞培养、切片技术、小鼠行为分析、小动物外科手术以及序列数据库和分析工具等生物信息工具的研究。

JPYFHY Recipe can inhibite the tumor of lung cancer transplanted animals, and the JPYFHY Recipe drug-serum can block the prolife ration of A549/DDP in vitro by inducing cancer cell apoptoses and boking cell cycle; It can enhance the chemotherapy synergism effects when they are used in combination and reverse multidrug resistance which because of DDP. The mechanism of these reversing drug resistance maybe is related to downregulating the expression of MRP and GST and upregulating the expression of TopoⅡ.

健脾益肺化瘀方对动物移植性肺癌具有抑瘤作用，能阻滞肺癌细胞进入增殖期，从而达到抑制肺癌细胞生长的目的；并能增强DDP的敏感性和具有对DDP的耐药逆转作用，其作用机理与健脾益肺化瘀方抑制A549／DDP耐药基因MRP和GST的表达和增强TopoⅡ活性密切相关。

Objective Cultured RK13 and Tn-5B1 animal cell lines were induced with heat-killed Esherichia coli DH5α or Staphylococcus sarophyticus.

目的 利用诱导的离体动物细胞系兔肾细胞RK13和昆虫细胞Tn-5B1产生抗菌活性肽类物质，筛选具有抑菌作用的抗菌药物。

Methods Fifteen healthy, female SD rats were employed in the experiment. Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth. Adminstrated 5-bromo-2′-deoxyuridine intraperitoneally, the rats were sacrificed and the temporal bones were harvested at 3, 7,14 day after labyrinth vaccination respectively. The frozen sections of the decalcified samples were dealt with H-E staining and immunohistohemical methods to investigate the cellular infiltration, BrdUrd and IgG positive cells in the ES.

选用SD大鼠15只，以钥孔虫戚血蓝蛋白（keyhole limpet hemocyanin，KLH）全身免疫后，经耳蜗底回钻孔以相同抗原进行内耳免疫，然后分别在内耳免疫后3、7和14 d腹腔注射溴脱氧尿嘧啶核苷（bromodeoxyuridine，BrdUrd）后处死动物，取颞骨经组织学处理制作冰冻切片，用免疫组织化学技术观察内淋巴囊的细胞浸润、增殖和IgG细胞的分布状况。

PartⅡExperimental Study on Acute Hypatocyte Necrosis and Apoptosis with MRI, DWI and MRS Objective: To demonstrate the ability and value of MR imaging, diffusion-weighted imaging, and MR spectroscopy in detecting and characterizing necrosis and apoptosis in vivo with a comparison to the pathology after establishment of hepatic necrosis and apoptosis animal model by different surgical ligations of hepatic blood supply vessels.

目的：通过手术结扎的方法建立大鼠肝细胞坏死和凋亡的动物模型，将不同时间点的MRI、DWI和MRS检测结果与病理学所见进行对照研究，以评价MRI、DWI及MRS在检测及鉴别肝细胞凋亡和坏死两种病理改变的能力与价值。

Results The histological observation of the cerebellar tissue in the animal experiment revealed that embryonic grafts could survive and that mitoses of the host Purkinje cells of the implanted group were increased substantially over the control groups. The short-term therapeutic effects were good in the 6 patients following cerebellar transplantation.

结果 动物实验的组织切片证实移植物在受体脑内再生，并且可见浦氏细胞分化成熟和迁徙到颗粒细胞层。6例患者接受小脑移植后近期疗效良好，症状和体征及客观指标均有改善。

In addition, we studied the distribution of mitochondria in metaphase Ⅱ rabbit oocytes, zygotes and early embryos by two active mitochondria dyes and revealed the change of active mitochondria during early development of rabbit embryos. We also found that the distribution of microfilaments and microtubules have similar patterns to that of active mitochondria, which is an important basis for further development of animal cloning study using the recipient oocytes derived from rabbit.

此外，我们应用两种线粒体荧光染料研究了日本大耳白兔体内成熟卵母细胞、正常交配后收集的受精卵及早期体外发育胚胎的活性线粒体的位置变化，首次揭示了兔早期胚胎中活性线粒体的变化特征及其与微丝微管变化的相关性，为进一步开展以兔卵母细胞作为核移植受体的动物克隆研究打下了重要的基础。

For the first time, we found that the mosquito was a transmitted infectious media of malignant cells to human and quadrumana. The mosquito's body was full of malignant cells, and transmitted pathway was that the mosquito first spit out malignant cells from its thrusting tube and into human body, then it sucked the blood from human body into its stomach.

本文首次公开垦表，钻研发明蚊子是人类和灵长类动物恶性肿瘤传染媒介，蚊子刺针管内充满恶性肿瘤细胞，蚊子在吸血时入针后须先把针管内的肿瘤细胞吐出，然后才顺利把血液吸入胃内。

The journal publishes original research on the evaluation and characterization of teratogens, carcinogens, or mutagens; reports describing the development of novel experimental techniques for toxicological assessment, or the application of current methods in molecular and cellular biology (including in vitro studies, organ and cell culture, and the use of transgenic animals); as well as critical reviews focused on topics of significant interest to specialists in teratology, cancer biology, and mutation research.

杂志刊有关载致畸剂，致癌物质和诱变剂的评估和特征方面的原始研究；描述毒物评定的实验新技术，或当前方法在分子和细胞生物学（包括体外研究，器官和细胞培养，和转基因动物）中应用的报告；以及对畸形学，癌生物学，和突变研究中生物学家非常感兴趣课题的评论。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。