动物细胞

Results In the control animals, the expression of the hsp70-like protein appeared only in the spiral ganglion, whereas in the cochleae with CIEAg immunization, strong expression of the hsp70-like protein and its mRNA appeared in the spiral ganglion as well as in the stria vascularis and the spiral ligament. The hearing thresholds were significantly increased in 10 out of 28 cochleae (35.7%) with CIEAg immunization.

结果 对照组豚鼠螺旋神经节细胞中存在hsp70样蛋白基础表达；实验组28耳中10耳（35.7 ％）听阈提高≥10 dB；此组动物螺旋神经节细胞和血管纹、螺旋韧带hsp70及其mRNA表达明显增强。

Five hours after the noise exposure, the cochleae were harvested. mitochondria energetic function, succinate dehydrogenase activity, in the hair cells of cochleae was evaluated with a colorimetric assay using blue tetrazolium monosodium salt. the sdh-labeled organs of corti were double stained with propidium iodide, a dna intercalating fluorescent probe used to visualize the morphologic viability of hair cell nuclei.

采用琥珀酸脱氢酶（succinate dehydrogenase，sdh）染色法进行耳蜗基底膜细胞线粒体染色，细胞核dna荧光染料碘化丙啶（propidium iodide，pi）双重染色耳蜗基底膜，以未受噪声暴露动物为对照，显微镜下观察噪声暴露后耳蜗基底膜核固缩和核肿胀毛细胞琥珀酸脱氢酶染色的变化。

Therefore, we detect the effects of A. fumigatus on airway epitheliums using animal asthma model and cultured cells in vitro, in order to explore in further the possible mechanism of A. fumigatus on the onset and development of asthma. Chapter 1 Effects of A.

本实验从动物哮喘模型和体外细胞培养两个方面阐述烟曲霉菌对呼吸道上皮细胞的作用及机制，进一步阐明烟曲霉菌在哮喘早期致敏和加重发病中的作用机制。

Histological observation showed that degeneration,necrosis of liver cells,edema,extravasated blood,erosion and degranulation of the mast cells in intestine mucosa.

肠谷氨酰胺酶活性降低，肠粘膜对谷氨酰胺利用减少，肠粘膜通透性增加。组织细胞学显示，TAA组动物肝细胞发生严重变性坏死；肠粘膜淤血、水肿、糜烂，肠肥大细胞脱颗粒。

Cytosolic calcium homeostasis can be ad justed by taurine,and the slightly increase cytosolic calcium in neuronal cells may be one of the major mechanism of taurine's enhancing the memory and learn ing ability.

牛磺酸对细胞内钙离子有双向调节作用，牛磺酸可能是通过对钙稳态的调节作用来阻止细胞内钙超载，保护神经细胞、并发挥其增强动物学习记忆等方面功能的。

Cytosolic calcium homeostasis can be ad justed by taurine,and the slightly increase cytosolic calcium in neuronal cells may be one of the major mechanism of taurine's enhancing the memory and learn ing ability.

牛磺酸对细胞很痛内钙离子有双向调节作用，牛磺酸可能过敏是通过对钙稳态的调节作用来阻止细胞很痛内钙超载，保护原则神经细胞很痛、并发挥其增强动物学习记忆等方面功能的。

This product can increase immunity of livestock and poultry, enhance the activity of phagocyte, which otherwise can not be performed by normal microorganism flora parasitized in animal intestinal tract.

禽畜机体内正常的微生物菌群对机体的免疫系统具有重要的诱发作用，本品可提升动物肠内免疫系统细胞，使一般非特异免疫功能得到协调和提高，加大吞嗜细胞的活性，这些并非原宿于禽畜肠道内微生物菌系所能达到的。

The thymuses and spleens were isolated from rats of different groups,and grinded into mononuclear cells on stainless steel mesh to analysis the phenotypes by flow cytometry at different time point.

各组在不同时间分别取出实验动物的胸腺和脾脏研磨成单细胞悬液通过流式细胞仪检测T细胞表型变化。

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清，进行以下检测：(1)原子吸收法测血清锌浓度；(2)测胸腺、脾脏脏器重量并与大鼠体重比较，计算脏器指数；(3)HE染色对胸腺、脾脏进行病理观察；(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡，并通过图像分析进行比较；(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达；(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达；(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

In order to verify the Fluorescent-Quantitation PCR kit specific for Borna disease virus nucleoprotein, compare molecular beacon with common probe and study its practical application, the sensitivity, distinctness, repeatability and stability were tested on BDV and non-BDV infected cells, as well as non-infected controls. Human and animal samples were also tested by the kit. The lowest quantity of virus RNA detected by the kit was 2.5×101, which corresponded to 1 virus copy.

为评价博尔纳病病毒(Borna disease virus， BDV)核蛋白荧光定量PCR试剂盒的各项指标，比较分子信标探针相对普通探针的优势，并了解其实际检测效果，本课题组使用BDV OL持续感染细胞株、非BDV病毒序列转染的OL细胞、正常的OL细胞，对BDV RT-PCR试剂盒的敏感性、特异性、重复性和稳定性进行评估，同时检测部分临床病人和动物外周血液RNA。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。