动物细胞

Rat in Group B was set an air bladder which was empty and not perfused water. Water of 0.6 ml, 0.8 ml, 1.0 ml, 1.2 ml, 1.4 ml and 1.5 ml was perfused respectively into the air bladders which were set in the colon of rats in group C, D, E, F, G and H. Then the changes of ethology of the rats were recorded in interval of an hour.

应用疼痛行为学的观察方法，观察1h各组动物的行为学改变，并采用免疫荧光化学的方法，观察大鼠DCN中神经元被激活的标志c-fos及胶质细胞的胶质原纤维酸性蛋白(glial fibrillary acidic protein， GFAP)和小胶质细胞特异性补体OX42的表达情况，空白对照组是在疼痛高峰点(1.2 ml)处死取材。

Epithelial cells and muscle is connected to a primitive phenomenon, Hydrozoa in some hard-jellyfish and jellyfish as well as most bowl Anthozoa animals, epithelial cells can be no contraction of the above-mentioned processes.

上皮细胞与肌肉相连是一种原始现象，水螅纲中的有些硬水母以及大多数钵水母和珊瑚纲动物，上皮细胞没有上述能收缩的突起。

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

In order to investigate the changes of HSF2 mRNA expression and its role in germ cell apoptosis induced by heat stress or a high of testosterone undecanoate,we established the operation induced unilateral cryptorchid and TU injected monkey models,and examined in situ analysis of testicular cell DNA fragmentation and the expre ssion level of HSF2 mRNA in germ cells after treatment.

为了探讨HSF2mRNA在热应激和超生理剂量睾酮诱导恒河猴生精细胞凋亡中的表达变化，我们建立了手术诱导单侧隐睾和注射大剂量 11酸睾酮恒河猴动物模型，应用 3′末端标记分析和原位杂交方法，检测睾丸细胞的凋亡信号和HSF2的表达变化。

Astrocyte Cell culture Cerebral cortex Ectogenesis Model ; animal Rat

星形细胞；细胞培养；大脑皮层；体外发生；模型；动物；大鼠

Collagen, a most abundant protein, is widely distributed in skins, tendons, cartilages and constitutes about 25%-30% by weight of all proteins in human or other animal bodies. Collagen structure is characterized by containing domains with repetition of the Gly-Xaa-Yaa prolinrich tripetide and the trimeric collagen triplehelices.

胶原是动物体内含量最多、分布最广的蛋白质，广泛分布于结缔组织、骨骼、内脏细胞间质及肌腔、韧带、巩膜等部位，占人体或其他动物体总蛋白含量的25％～33％。

To observe the expression of α-MSH in four different animals scargskin and to study its meaning for finding a novel evidence to research zoopery of pigment disease.

［目的］初步研究α-黑素细胞刺激素(α-melanocyte-sti mulating hormone，α-MSH)在不同实验动物表皮中的表达并阐明其意义，为色素障碍性皮肤病动物实验提供新的理论依据。

Methods Fifty white guinea pigs with normal hearing were randomly divided into the control group, ligustrazine group and traditional Chinese drug group. The alternations of hearing function were evaluated by the examination of auditory brainstem response. Some specimens were perfused by AgNO3 and observed the damage of bristle cell. And some were sent for immunohistochemistry to detect the expression of iNOS. Results Compared with the control group, the threshold of ABR in ligustrazine group and TCD group were tignificantly different.

将50只健康白色红目豚鼠随机分为对照组、顺铂组和中药组，各组动物均于用药前及停药后测试听觉脑干诱发电位，采用耳蜗铺片观察毛细胞损伤情况，应用免疫组织化学方法测定各组动物耳蜗内iNOS的表达情况。

A low number of algal cells in the presence of a high number of grazers suggested, but did not prove, that the grazers had removed most of the algae.

一个海藻细胞的低数字在一个吃草的动物高数字之前建议，但是没有证明，吃草的动物移动大部份的藻类。

Most animals, including all the shelly Cambrian ones, grow from three-layered embryos.

而包括所有寒武纪壳类动物在内的大多数动物都是由三层细胞的晶胚发展而来。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。