动物细胞

Objective To establish an animal model of asphyxial neonatal rat and compare the effects of resuscitation with 100% oxygen and resuscitation with room air on the ultrastructure and the apoptosis on the kidney of asphyxial neonatal rats,in order to provide a scientific basis for clinical choice to resuscitation.

目的 建立窒息新生大鼠动物模型，比较纯氧与空气复苏对窒息新生大鼠肾组织超微结构和细胞凋亡的影响，为临床选择使用纯氧或空气复苏提供动物实验依据。

In order to develop an accurate and efficient method for the identification of tiger DNA,mtDNA Ctyb sequences of 5 tiger subspecies and 6 other feline species and 6 cervid species were downloaded from Genbank,and compared by Wdnasis(V2.5) software.

为了建立对虎DNA进行特异性检测的有效、精确的方法，本研究中，从Genbank下载虎及其他6种猫科动物和6种鹿科动物的mtDNA细胞色素b基因序列，并用Wdnasis(V2.5)软件进行了种间序列比较。

Based on this,We designed a series of experiments to study the treatment and its mechanism of Iridoids composition of Valeriana jatamansi Jones of the IBS animal model.1 Research on Iridoids composition of Valeriana jatamansi Jones in the role of gastrointestinal function.1.1 Effection of Iridoids composition of Valeriana jatamansi Jones on gastrointestinal function of normal miceMethods:Compared with normal group and YJBH group,mice which has given Iridoids composition of Valeriana jatamansi Jones as 71.2mg/kg,35.6mg/kg,17.8mg/kg,8.9mg/kg was detected gastric emptying and small intestinal propulsion after 4 days drug given.Results:Compared with normal group,gastric emptying and small intestinal propulsion in 35.6mg/kg and 17.8mg/kg group was inhibited.

课题通过观察蜘蛛香环烯醚萜对正常及IBS模型动物的胃肠道功能、精神活动的影响，以及蜘蛛香环烯醚萜干预下IBS大鼠结肠、血清、中枢5-HT含量，结肠5-HTR3A受体的mRNA和色氨酸羟化酶(TPH1)的mRNA表达，结肠和血浆中SP和VIP含量以及结肠中肥大细胞分布，探讨蜘蛛香环烯醚萜对腹泻型IBS的治疗作用及其作用机理，为临床治疗IBS提供实验依据。1蜘蛛香环烯醚萜对胃肠功能的作用目的：探讨蜘蛛香环烯醚萜对胃肠功能的影响1.1蜘蛛香环烯醚萜对正常小鼠胃肠功能的作用方法：正常小鼠灌胃给予蜘蛛香环烯醚萜71.2mg/kg、35.6mg/kg、17.8mg/kg、8.9mg/kg与空白组和越鞠保和丸组对照，给药4日后测定动物的胃排空率和小肠推进率。

Methods and Results— Using a micro–single-photon emission computed tomography small-animal imaging system and a Food and Drug Administration–approved radiotracer ([indium 111] oxyquinoline, 111In-oxine), we demonstrate here that monocyte recruitment to atherosclerotic lesions can be visualized in a noninvasive, dynamic, and 3-dimensional fashion in live animals.

方法和结果——采用微单光子发射计算机断层摄影术的小动物成像系统和美国食品药品治理局认同的放射性示踪剂（111In-oxine），我们证实动物体内单核细胞向动脉粥样硬化损害处的聚集能够通过一种非侵入性，动态的，3维方式进行可视化观察。

In the study, we established the animal model of diabetic ED successfully. Modern medical methods such as routine lightscopy, pathology, transmission electroscopy, scanning electroscopy, fluorospectrophotometer and radio-immunity were employed. From the aspects of pathomorphism, cellular supermicrostucture and neurocrine, we observed the mixture's effect on status of the whole animal model and penial erection, the fast plasma glucose, protein non-enzyme gycosylation, nitric oxide synthase , testosterone. In contrast with control groups, the mixture could apparently slow down the corpus cavernosum penial, vascular, neural pathologic progression of the diabetic rats and had a better result than diabetic pill, invigorating yang and restoring normal capsule and amino guanidine.

通过实验研究，成功建立了糖尿病性阴茎勃起功能障碍动物模型，利用常规光镜病理、透射电镜、扫描电镜、荧光分光光度计、放射免疫法等现代医学研究方法，从病理形态学、细胞超微结构、神经内分泌学等方面，观察了降糖起萎合剂对模型动物整体状态和阴茎勃起情况、空腹血糖水平、蛋白非酶糖基化、一氧化氮合酶、睾酮等实验指标的影响，与对照组比较提示降糖起萎合剂能明显延缓大鼠基于DM基础上的阴茎海绵体及血管、神经病变的病理进程，其疗效优于消渴丸、壮阳复春灵胶囊及氨基胍。

The results are: 1 the stricture of the capillaries of stria vascularis was observed in most cochleae of rickety rats and, segmental deletions of the outer hair cells on the first turn of cochlea were found in a small number of rickety rats; 2 the perilymphatic ionized calcium concentration significantly re- duced and the threshold of CAP (Compound Action Poten- tials of the eighth nerve) significantly rose in rickety rats in comparison with those of normal control; 3 gentamicin results in an increase of the perilymphatic ionized calcium concentra- tion in rickety rats; 4 the cochleae of the rickety rats appeared to be less vulnerable to the ototoxicity of gentamicin than the normal one.

研究包括两个方面，其一是&佝偻病的感觉神经性耳聋及其对庆大霉素耳毒性作用的耐受性&，系用大白鼠建立维生素D缺乏性佝偻病动物模型，发现：（1）佝偻病大白鼠耳蜗血管纹毛细血管狭窄，少数动物耳蜗底回外毛细胞有节段性缺失；（2）佝偻病大白鼠的耳蜗鼓阶外淋巴钙离子浓度降低，CAP（听神经复合动作电位，以下简称CAP）听阈提高；（3）庆大霉素可使佝偻病大白鼠耳蜗外淋巴钙离子浓度上升；（4）佝偻病大白鼠耳蜗在功能和形态上都比正常者更能耐受庆大霉素的耳毒性损害。

Objective To study the statement of immunoreaction of c Jun and functional recovery of spinal cord injury in rats after fetal spinal cord transplantation.Methods Experimental rats were divided into two groups:spinal cord hemisection injury combination with transplants of FSC to injured site group,and spinal cord injury that received gelfoam pledgets group.On the lst,3th,7th,14th and 28th day after operation,6 animals in each group at each time phase,were observed for their function recovery with the methods of praxiology and electro physiological examination,the animals were then sacrificed for the observation of the statement of c Jun reaction by immnochemistry method and computer image analysis system was used for quantitative analysis.

目的：研究大鼠胚胎脊髓移植后能否影响，c-Hun免疫反应的表达和脊髓损伤后大鼠功能的恢复，方法；将动物分为脊髓半切洞损伤加胚胎脊髓移植组和单纯脊髓板切洞损伤加明胶海绵填塞组，每个时相点每组6只动物，术只1，3，7，14和28天，应用行为学和电生理检查观察大鼠功能恢复情况，应用免疫细胞化学方法，c-Jun免疫反应的表达，采用计算机图像分析技术，进行定量分析。

This paper mainly investigated the effect of maternal undernutrition during late pregnancy on the levels of hormones which take the somatotropic axis as the center in ewes and lambs, the expression of partil hormones'mRNA and the gene localization in tissue cells on the basis of animal nutrition, endocrine physiology and by modern molecular biology technique.

中文摘要：本研究依据动物营养学和内分泌生理学理论，应用现代分子生物学技术对限制营养的妊娠母羊和羔羊以内分泌生长轴为中心的激素水平变化和部分激素及其受体mRNA表达水平的影响以及在组织细胞中基因表达定位规律等进行研究，进一步探究反刍动物补偿生长的机理，为更科学地将动物的补偿生长能力应用于畜牧业生产上奠定理论基础。

This study mainly investigated the effect of maternal undernutrition during late pregnancy on the levels of hormones which take the somatotropic axis as the center in ewes, the expression of partil hormones'mRNA and the gene localization in tissue cells on the basis of animal nutrition, endocrine physiology and by modern molecular biology technique. The experiments explored the mechanism of restriction and compensatory in order to pave the way for applying compensatory growth for animal husbandry scientifically.

本研究依据内分泌生理学和动物营养学理论，应用现代分子生物学技术系统研究了限饲及补偿生长对母羊生长轴激素基因表达水平的影响以及部分激素在母羊组织细胞中基因表达定位规律，进一步探究了绵羊补偿生长与内分泌生长轴的关系，为更科学地将动物的补偿生长能力应用于畜牧业生产上奠定理论基础。

In the experimental study section, the rats were divided into four groups, including the normal control, the model, the Piracetam, the QPC, and copied to AD model. After the treatment, we observed the effects on the AD rats' ability of leaning and memorizing, detecting the TChE activity in whole blood and the expression levels of the related biochemical indicators of As includeing GFAP、NGF and BDNF of the AD rats' brain hippocampus, then compared the QPC with other groups to reveal the mechanism of action of the QPC treating AD to bright.

在动物实验部分，我们通过塑造AD动物模型，设立正常对照组、模型组、脑复康组和强脾益智胶囊组，观察强脾益智胶囊对AD模型大鼠空间学习记忆能力的影响，检测AD大鼠全血、海马匀浆液中乙酰胆碱酯酶活性和通过免疫组化的方法检测胶质纤维酸性蛋白、神经生长因子、和脑源性神经营养因子的表达情况来反映星形胶质细胞的增值情况，以期揭示强脾益智胶囊对AD的治疗作用机理。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。