动物细胞

One embryonic cell can be separated into many blastomere cells. As be the nuclear donor of the embryonic cell nuclear transfer, it can breed the number of eminent species largely, increase the number of endangered animals, supply large number of supply same genotype animals, improve the efficiency of gene transfer and sex control and offer same genotype apparatus or organ for human transplant.

一个早期胚胎细胞可以分离多个卵裂球，以此作为核供体进行胚胎细胞核移植，可以大量扩繁优良品种的数量，增加濒危动物的数量，为科学研究提供大量遗传同质的动物，提高转基因和性别控制技术的效率，提供基因型相同的器官或组织供人类移植。

Animal growth is the course of cell division and protein synthesis, it is regulated by endocrine growth axis of brain, animal growth fast or slow is depended on concentration equilibrium of growth hormone and Somatostatin in blood.

动物生长发育是细胞分裂与蛋白质合成的结果，它由脑垂体神经内分泌生长轴所控制，动物生长速度的快慢最终是生长因子与生长抑制因子平衡的结果。

Methods On the basis of bilirubin induction and NMDA receptor an-tagonist GAPA administration in guinea pigs. The brain extracellular EAA neurotransmitter was ex-tracted with microdialysis and analysed with HPLC; then, brain tissue ATP was measured by fluores-cence assay and intracellular Ca2 + was measured by fluorescence imaging analysis.

制作胆红素脑病动物模型基础上，在体脑内微透析提取兴奋性氨基酸神经递质，HPLC检测天冬氨酸、谷氨酸、甘氨酸，并给予模型动物NMDA受体拮抗剂GAPA干预，电镜检测脑组织超微结构，荧光检测脑组织ATP与细胞内Ca2+含量。

Prove that the soap glucoside of ginsengs is a kind of very good immunity strengthens the pharmaceutical through animals experiment, It is strengthening the systematic function of single-nuclear macrophage cell, promotes the formulation antibody and lymphocyte transformed, Promote some lymph secretion of factor, play a regulating role in natural killer cell-interferon-IL-2 regulate network, and protect the organization cell attacked by the virus.

通过动物实验证明人参皂甙是一种很好的免疫增强剂，它具有扶正固本、增强单核巨噬细胞系统功能，促进特异性抗体的生成和淋巴细胞转化，促进某些淋巴因子的分泌，对天然杀伤细胞-干扰素-白介素-2调节网起正调节作用，并能保护受病毒侵袭的组织细胞。

The cerebellum consists of five distinct concentric layers: white matter, internal granule layer, Purkinje cell layer, molecular layer, and external granule layer in young rodents.

在年幼的啮齿类动物中，小脑包含5种不同的集中分布层：白质、内颗粒细胞层、浦肯野氏细胞层、分子层和外颗粒细胞层。

Results: The animal experimental model indicates the facial nerve' s bone canal fracture can make the facial nerve hyperaemia, strut, and putrescence, denaturalization and with the Schwann cell and the cell become more and more, taking on the restore evidence.

结果 动物实验表明：面神经骨管骨折后，面神经发生充血肿胀，并出现脱髓鞘、变性及坏死的变化，伴随神经损伤的同时出现巨噬细胞和施万细胞增生肥大及纤维母细胞的增生，呈现修复迹象。

For the purpose of constructing mammary gland specific expressional vector, we use the updream regulation sequence of bovine BLG to regulate lacS gene to express in mammary gland. Positive clone cells are selected and purified by the expression Neo gene and report gene EGFP. So the cells can be used as sources to create transgenic animals by nuclear transfer techniques.

本研究以牛β-乳球蛋白基因上游调控成分作为启动子，嗜热菌糖苷酶基因lacS为目的基因，构建了lacS基因乳腺特异表达载体，并通过脂质体介导法转染体外培养的牛胎儿成纤维细胞，借助新霉素抗性基因和增强型绿色荧光蛋白报告基因来筛选、纯化阳性克隆细胞，为利用核移植技术制备转基因动物提供细胞来源。

The result showed that microcells containing our interest chromosomes-human chromosome 12 were equally distributed in the preparation, the preparation was suitable for use in generation of transchromosomic animals.

微细胞PCR技术首次用于检测微细胞溶液的质量，检测结果显示，所制备的溶液中均匀分布着带有目的染色体的微细胞，适用于进一步作转染色体动物实验。

Methods The rat thoracic duct endothelia were cultured and the morphology observed and identified by TEM and Factor Ⅷ-related antigen immunohistochemically. Wistar rats were used to make the animal model of primary colorectal cancer, the cancer type were appraised by HE staining and PAS Reaction. The expression of VEGF-C and its receptor Flt-4 and ICAM-1 in the cancer tissues were carried through immunohistochemistry staining and In situ hybridization.

通过体外培养大鼠胸导管内皮细胞和构建大鼠原发性大肠癌动物模型，观察内皮细胞的形态和生长状态，用透射电镜和Ⅷ因子相关抗原进行鉴定，用HE染色和过碘酸雪夫氏反应区别癌组织的类型和期别，用免疫组织化学方法和原位杂交方法研究内皮细胞和大肠癌组织的VEGF-C及其受体Flt-4和ICAM-1的表达。

In our research,Wistar rats and SD rats which were parathyroidectomized were used as donors and recepients respectively.The parathyroid gland of the Wistar rat were allografted into the cerebroventricle and other different sites of the brains in SD rat s tereotaxically. Using methods of testing serum Ca~(2+) concentration,histological examination,histochemical and immunocytochemical methods and electron microscopic observation,we investigated the survival of the grafting tissue,its revascularization, infiltrating of monocytes, changes of microglia and astrocytes, the relationship of the CNS with the immune system, and the mechanism of rejection were investigated.The results as following: I .Effects on PTG after allografting into cerebroventricle and cerebral parenchyma of the brain: Testing serum Ca~(2+) conc.

本研究以Wistar大鼠作为供体，切除甲状旁腺的SD大鼠作为受体，在立体定位下将同种异体的PTG移植到大脑的不同部位，设计了侧脑室-内分泌系统移植的代替途径，比较了侧脑室内移植和脑实质内移植的异同，通过测定血清Ca~(2+)的浓度、组织学检查、组织化学和免疫细胞化学以及电镜观察对移植后PTG组织的存活、血管的再生、单核细胞的浸润、CNS内胶质细胞的变化、对免疫系统的影响及免疫排斥的机制进行了研究，结果如下：一、同种异体PTG侧脑室内移植与脑实质内移植的比较：血清Ca~(2+)的检查发现，PTG侧脑室内移植后第7天，血清Ca~(2+)的浓度恢复到8mg％以上，一直持续到移植后第8-12周；移植后第12周仍有60％移植PTG的动物血清Ca~(2+)维持在正常水平。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。