动物细胞

Zeta potential was -44.23 millivolt. Viscosity was 1.83 mpa. All of which were coincided with physical desire. In addition, the tests of PHGF liposomes including irritation, hemolysis, pyrogen, anomaly toxicity and hypersensibility, proved that the preparation reached the standard of intravenous injection entirely. Taking glutamic pyruvic transaminase and malondialdehyde in mice as index, studies on pharmacodynamics after hepatic injure caused by tetrachloromethane in mice indicated that GPT level (P.001) and MDA yieldment P

以小鼠CCli急性中毒性肝损伤为模型，以动物血清中谷丙转氨酶（GP水平与肝脏中丙二醛p A烨成量为指标，进行PHGF脂质体的药效学试验，结果表明P＊＊F脂质体能显著降低GP T水平o＜0刀01）与MD A 生成量厌＜o.05）；大鼠组织形态的观察表明高剂量＊＊＊F脂质体能使肝脏 0 内皮细胞增生活跃，提高肝细胞的免疫功能，对受损肝细胞具有修复作用；同样小鼠碳廓清试验也显示PHGF脂质体较市售粉针具有更好的机体免 p 疫作用o功刀01），达到制剂设计目的，获得较好的结果。

The first description of Pulmonary Neuroendocrine cells dates back over 60 years when single and small clusters of cells with argyrophilic cytoplasm (stainable with silver impregnation method) were described within the airway epithelium of human and animal lungs.

第一次对肺神经内分泌细胞的说明要回顾60年以前，单一的小细胞簇和嗜银细胞质在人类和动物肺气道上皮细胞中被描述。

The translationally controlled tumor proteingene of Plasmodium falciparum recombined with the pQE-30 vector was transformed into the E.coli M15 and induced with IPTG to express TCTP.

翻译控制肿瘤蛋白最初在小鼠肿瘤细胞中被发现，是一个受翻译过程所阻遏并与肿瘤生长相关的蛋白质，随后在酵母及植物和除肾细胞外的各种动物组织正常细胞中被发现(Sanchezet al。，1997)。

FACS analysis for the trimera model one week later showed that the engrafted human cells in peritoneal cavity of trimera held 88.84% of total peritoneal cells.

免疫组化结果显示，在实验组的脾脏中有大量的人CD20〓细胞，CD3〓细胞则散在分布于其中。各种疫苗免疫该模型10天后，结果显示，用照射过的肿瘤细胞免疫动物在E/T为100时的特异CTL杀伤率为40％左右。

In conclusion, like human mast cells, bullfrog mast cells also contain the special enzyme, tryptase and it was likely that bullfrog can be used as an ideal experimental animal for the study of mast cell biology.

研究证实，与人类肥大细胞相似的是牛蛙肥大细胞也含有这种特有的类胰蛋白酶，牛蛙有可能是用作肥大细胞生物学研究的理想实验动物。

Methods: NG108-15 cells were cultured in vitro at 37℃in a humidified atmospherecontaining 5％CO_2. Aβ_(25～35) fragment was dissolved with deionized distilledwater and aged for 4 days at 37℃. The cells were exposured to 5μmol/LAβ_(25～35) for 24h to establish the cell model. Twelve rabbits were divided intotwo groups at random, and garaged with MWD or physiological saline controlrespectively for 7 days to collect two kinds of cerebrospinal fluid, theyare CSF from rabbits treated with MWD and CSF from rabbits treated withphysiological saline.

NG108-15细胞在37℃、体积分数为5％的CO_2培养箱中培养，Aβ_(25～35)溶解于双蒸水中，置于37℃培养箱中老化4d，使其寡聚，毒性增强，细胞被置于浓度为5μmol／L的Aβ_(25～35)中作用24h，建立起AD细胞模型。12只清洁级大耳白家兔购于华中科技大学同济医学院动物中心，将其随机分成两组，分别给与等量的加味温胆汤和生理盐水灌胃7d，末次给药后1h内用水合氯醛将给药组白兔麻醉，1.2ml／kg，无菌条件下用7号注射针头从枕骨大孔处垂直进针，抽取清亮含药脑脊液。

The coil spring were kept constant and recorded for 0hr, 1 hr, 2 hrs, 4 hrs, 8 hrs, 12hrs, ldays, 3days or 7days. Upon completion of experiments, the maxillae were removed. The specimens were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer for 24 hrs and decalcified in 10% ethylene diamine tetraacetic acid at room temperature for 5 wks.

本研究通过建立正畸大鼠牙齿移动动物模型和体外细胞培养，结合细胞力学试验方法和分子生物学测试技术，拟从组织、细胞水平以及分子生物学角度探讨ATF4在正畸牙齿移动过程中对牙周组织改建的影响及作用机制，进一步为正畸牙齿移动提供理论和实践依据。

Animal size;control mechanisms;cell number;cell division;cell death

动物体型；调控机制；细胞数量；细胞分裂；细胞死亡

Methods After Candida albicans were inoculated into the specific pathogen free mice, the total amount of Candidas in cecum and the amount of which attached to the mucosal membrane were counted at different interent intervals. The lymphocyte proliferation in Peyer's patch and in lamina proper was shown by BrdU incorporation, meanwhile B cells isotype switch in PP was investigated. IgA plasma cells were shown by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.

采用无特殊病原菌动物经口喂入白念菌，在不同时相处死后，观察肠内白念菌总数及肠粘膜表面白念菌粘附数量；取肠系膜淋巴结做白念菌选择培养，观察移位体内发生率；采用Brdu体内掺入显示局部粘膜淋巴细胞的增殖情况；流式细胞计数潘伊尔氏结细胞表面IgA阳性率；免疫组化染色后计数固有层中IgA浆细胞数量变化；ELISA法测定肠粘液中特异抗白念菌IgA含量。

Compared with the control, the lungs of 3-day-hyperxia-exposure group developed into edema, hemorrhage and inflammatory infiltration and this condition aggravated at the 7th day. The pulmonary septum was significantly widened at the 14th day, the interstitial fibrocytes as well as alveolar epithelial cells proliferated obviously, and the tendency of lung fibrosis was also presented. The results mentioned above showed that the modle of hyperoxia lung injury was successfully established, and the acute inflamationm was its early symptom while the fibrosis was its late manifistation. There was no positive cytes of IFN-γ in the lung of nomal rats, The positive cytes of the groups under hyperxia-exposure for 3, 7, 14 day, which climbed to the climax at the 7th day and began to decline from the 14th day, were found in alveolus epithlia,pulmonary interstitial, bronchoepithlia and macrophage.

高氧14天（5.46±0.30）虽有所下降，但同空气组比，差异仍然具有显著性（P.05）；BALF中蛋白含量高氧3天（0.45±0.10）、7天（0.66±0.08）、14天（0.79±0.14）显著高于空气组（0.18±0.08，P值均。05）；肺通透系数高氧3天（6.61±2.05）、7天（10.88±1.54）、14天（12.75±2.20）均明显高于空气组（2.99±1.27，P值均。05）；同时病理组织学改变也表明与空气组比较，高氧3天时肺组织水肿、出血、炎性细胞浸润，7天时进一步加重，14天时肺间隔明显增宽，间质纤维细胞和肺泡上皮细胞明显增生，出现肺纤维化倾向；以上结果均表明我们成功地建立了高氧肺损伤动物模型，且急性炎症反应是其早期表现，纤维化改变是其晚期表现。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。