动物研究

We further evaluated the synergistic cytotoxicity of TRAIL in combination with actinomycin D, cisplatin and paclitaxel in A549 cell lines in vitro and in vivo.

本实验选择了人肺腺癌A549细胞瘤株作为我们实验研究的对象，通过TRAIL对人肺腺癌A549细胞诱导凋亡研究，在A549的荷瘤裸鼠的动物模型上研究了其体内的抗肿瘤活性，并进一步研究了TRAIL与化疗药物放线菌素D(actinomycin D，Act D)、紫杉醇和顺铂(cisplatin，CDDP)的联合作用对人肺腺癌A549细胞的体外协同抗肿瘤活性，通过化疗药物协同增强TRAIL抗肿瘤活性作用机制的初步研究，为降低临床化疗药的用药量，减轻其对机体的不良反应提供一定的理论基础。

Carabid beetle was selected as bioindicators and pitfall trapping was used as sampling method for animal biodiversity evaluation; for flora evaluation, both field experiment and releve approach were used with assistance of remote sensing image; for the evaluation of soil organic carbon change, a Regional Resources and environmental information system was established; and RS, GIS and biogeochemistry model were all used to evaluate the soil organic carbon change.

生物多样性研究中，动物多样性研究部分以步甲作为指示生物展开，方法为陷阱法；植物多样性研究部分采用田间试验以及遥感影像配合下的样方记录法；对区域土壤有机碳变化的研究采用遥感、地理信息系统和生物地球化学循环模型模拟相结合的方法。

PSO is a population-based optimization algorithm, which mimics the social behavior of animals in a flock. It makes use of individual and group memory to update each particle position allowing global and local search optimization. The objective function considered was the total weight of the structure subjected to stresses, displacements and forces constraints. The effects of the parameters were investigated as well and such combination of tuning parameters promote a better global search behavior avoiding premature convergence while rapidly converging to the optimal solution. Results showed the effectiveness of the proposed method by comparing with ANSYS Design Optimization Tool (zero-order method). The PSO with the tuning parameters makes it an ideal method for offshore wind turbines foundations optimization tasks.(2) A reliability analysis method for pile foundation bearing axial loads based PSOThe performance function of pile foundation's axially bearing capacity sometimes is nonlinear and complex, on the basis of geometric meaning of structural reliability index, an optimum model with PSO for structural reliability analysis under arbitrary random variables was established, The PSO algorithm is very efficient to solve global optimization problemsIts use in structural reliability field presents not only the advantage of its facility of implementation, but also the possibility to obtain the design point and the failure probability with a good accuracy. In addition, PSO is a zero order algorithm, for no derivative is necessary for its implementation.

本文的研究针对桩式海上风机基础结构的特点，在国内外有关研究成果的基础上对海上风机基础结构优化设计理论和可靠度方法一些相关问题进行了较为深入的研究，具体做了以下几个方面的工作：(1)基于粒子群优化的桩式海上风机基础确定性优化设计方法桩式海上风机基础的优化设计是一个复杂的、非线性约束的优化问题，针对传统的基于梯度信息的优化方法在处理非线性问题中易陷入局部最优解的问题，本文将一种耦合惩罚函数的PSO算法引入到海上风机基础结构概念设计中，PSO算法是从群体动物聚集觅食这一活动中受到启发而发展的，该算法利用个体和群体的信息共享不断改进自身的位置从而进行局部和全局最优搜索，本文中以桩和三脚架连接段直径及壁厚为设计变量，以基础总重量作为优化的目标函数，在给定的约束条件下建立了三脚架基础优化数学模型，另外本文还研究了PSO参数变化对结果的影响，协调的参数组合可以避免陷入早熟收敛而能够快速的获得全局的最优解，通过与ANSYS优化模块的计算结果比较验证了该方法的有效性，本方法为海上风机基础的确定性设计提供了一条有效的途径。

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型，以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型，进行"长动"的抗肿瘤药效学研究：用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究；用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究；用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响；用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

Observe the effect of Fuling Gancao Decoction on gastric liquid emptying and content of nitric oxide and acetylcholine esteras in the gastric fundus,antrum and duodenal tissu of functional dyspepsia rats and investigate the mechanism.1 Reference reviewThe reference review discussed the source of disease name of TCM for FD,etiology and pathogenesis of TCM,selection of therapeutic methods by different stages or according to the differential syndrome diagnosis,advance on the clinical and experimental research as well as the problems lying in the research of TCM and corresponding strategies.We also have summarized the recent research progresses on the FD.In the meanwhile,we further exploited the pathologic characteristics,diagnosis and treatment of FD;summarized etiology and pathogenesis of TCM for FD and application of Classical Prescriptions in clinical and experimental study;Collected and arranged the Literatures and summarized the recent research progresses of Fuling Gancao Decoction;Finally, collected and arranged the literatures of proved cases and clinical experiences of Fuling Gancao Decoction.2 Theoretical studyThe Theoretical study first discussed the source of TCM for stomach deficiency and congestion of fluid-retention in syndromes of FD;Secondly, summarized the indications of Fuling Gancao Decoction,that is:①epigastric throb,②cold hands and feet in syncope,③hydroadipsia,④gastric blockade,⑤gastric splashing sound,⑥no desire for drinks,⑦light white tongue,thin-white and smooth fur,⑧deep and wiry pulse are the indications of Fuling Gancao Decoction;Subsequently,analyzed the composition of Fuling Gancao Decoction,considered that ginger is the main drug which treating congestion of fluid-retention in Middle-Jiao by Zhang Zhongjing.3 Experimental study3.1 Experiment oneObjective:To establish the animal model of FD with delayed gastric liquid emptying and evaluat it.

同时观察茯苓甘草汤对FD大鼠胃液体排空及胃底、胃窦及十二指肠中乙酰胆碱酯酶、一氧化氮含量的影响，探讨茯苓甘草汤的作用机制。1文献综述参照古、今中医有关文献对FD的中医病名、病因病机、辨证论治方法、临床及实验研究进展进行了探讨，并对现代医学在FD研究中所取得的(来源：A0bBC论文网www.abclunwen.com)进展进行了总结，进一步明确了FD的发病机制、诊断和治疗的研究进展；总结了中医对FD的病因及发病机制、中医诊断及经方治疗FD临床及实验研究进展；并搜集和整理已发表的有关茯苓甘草汤研究报道，阐述了茯苓甘草汤的现代研究进展；最后搜集和整理了近现代著名医家应用茯苓甘草汤的经验及验案。2理论研究理论研究部分首先对功能性消化不良胃虚饮停证的认识进行了理论溯源；其次对茯苓甘草汤的适宜证象进行总结，认为：①心下悸，②四肢不温，③口不渴，④胃中痞满，⑤胃中振水音，⑥不欲饮水，⑦舌淡白，苔薄白而滑，⑧脉沉弦是茯苓甘草汤的主证；随后对茯苓甘草汤方药组成解析，认为生姜是仲景治疗中焦饮停的一味主药。3实验研究3.1实验一目的：探讨建立功能性消化不良胃虚饮停证的动物模型方法并对其进行评价。

A 2. 5～5. 5 MHz transducer was used according to the acoustic depth of the heart examined. Group B was used x-ray fluoroscopy (a Philip mono-rotatable C arm X-ray equipment) as image guide with routine method reported by american college of cardiology cardiovascular technology assessment committee. TTE guiding CSC in site: The guide wire was inserted into left subclavicular vein and inferior vena cava and it was displayed clearly by TTE. The sheath could be displayed clearly too by TTE. CSC was put through the sheath into right atrium in prone position and was thoroughly showed by TTE via untypical parasternal four-chamber view.

本研究对60例心内电生理检查患者，应用TTE作为影像学引导的可行性和临床应用的方法学进行了探讨，并对5只犬TEE引导射频导管消融术时大头电极导管到位的可行性进行了动物实验研究，具体为：(1)专用冠状窦电极导管置放时TTE引导的可行研究；(2)普通电极导管置放时TTE引导的可行研究；(3)专用冠状窦电极导管和普通冠状窦电极导管置放时TTE的对比研究：(4)TTE引导His束电极导管和右室电极导管到位的临床应用研究；(5)TTE在心内电生理检查中的应用研究；(6)TEE引导射频导管消融术大头电极导管到位的实验研究。

The coeve to the plasma study of monoamine neurotransmitter in normal person and schozophreniac on four mian solar term in four seasons; tostudy the amount and the component of middle molecule substances in plasma on four main solar term in four seasons in nor- mal person and schizophreniac; to estabisht the experimental animal model of sehijorhrenia and the improve the measurement index; to study monoamine neurotransmitter value of brain in normal rats and experimental schizophrenia rats on four main soler terms; to study the amount and component of MMS in plasma of normal rats and experi- mental schizophrenia rats on four main solar terms, and to make The morphological study of the brain tissue in experimental schizophre- nix rats and normal rats.

健康人、精神分裂症患者一年四季血浆单胺类神经递质含量的研究。2。健康人、精神分裂症患者一年四季血浆中分子总量及组分的研究。3。精神分裂症动物模型的建立及评分标准的改进。4。正常大鼠、精神分裂症模型大鼠一年四季脑组织单胺类神经递质含量的研究。5。正常大鼠、精神分裂症模型大鼠一年四季血浆中分子总量及组分的研究。6。精神分裂症模型大鼠、正常大鼠脑组织形态学研究。

In order to understand the expressed genes, construct the known gene expressed profile, study the tissue-specific genes and the common genes from pig and chicken; In order to do primary researches for comparative genomics, functional genomics, QTLs mapping, animal molecular breeding and so on, we have constructed 3 cDNA libraries of Xiang Pig muscle tissue, Silkie Fowl muscle tissue and liver tissue, sequenced a large scale ESTs and analyzed their bioinformation from the ESTs of Silkie Fowl muscle tissue and liver tissue in our study.

为全面了解肌肉组织和肝脏组织的基因表达类型，构建猪、鸡肌肉组织和肝脏组织的功能基因表达谱，研究肌肉组织和肝脏组织的特异表达基因及这两种组织的共同基因；为比较基因组学的研究、功能基因组学的研究、QTLs定位等的动物分子育种做前期基础科研工作，本研究构建了香猪肌肉组织、丝毛乌骨鸡肌肉组织和肝脏组织的3个cDNA文厍，并对丝毛乌骨鸡的肌肉组织和肝脏组织进行了大规模的ESTs测序及其生物信息学的初步研究。

One school of ethologists devoted to the study of animal behavior has it that human beings are unique in the possession of consciousness, differing from all other creatures in being able to think things over, capitalize on past experience, and hazard informed guesses at the future.

献身于动物行为研究的一家比较动物学者的研究所断言，在拥有意识方面人是独一无二的，在将事情深思熟虑，利用过去的经历，冒险地说出对于未来的猜想方面不同于其他动物。

By rigorous and clever experiments the three ethologists refined the lore of animal antics into a respectable science called ethology (roughly, the study of character). In 1973, they shared a Nobel prize for their pioneering achievements.

通过严谨而精巧的实验，这三位动物行为研究者把那些关于动物怪异行为的学问提炼成值得尊敬的学科，它的名字就叫做&动物行为学&（粗略说，就是研究特性的科学）。1973年，他们因为这些开创性的工作成就共同获得了诺贝尔奖。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。