动物的胃

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制备SD大鼠肝纤维化动物模型，连续灌胃6周，处死动物取血，检测血清ALT、AST、ALB；ELISA法测定血清透明质酸；取肝、睥，并计算肝、脾指数，组织切片HE染色后进行组织病理学疗效评估；用SP免疫组化法测定肝组织TGF-β1、PPAR-γ及PDGF蛋白表达；半定量RT-PCR方法检测肝组织TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γ和iNOSmRNA的表达。

Methods: Ligating pylorus method, acetic acid and reserpine were used to induce gastric ulcer models in rats and mice, respectively.

目的：观察比较胃益油剂、胃益糖片、胃益胶囊3种药物对动物实验性胃溃疡的影响。

Methods BALB/c mice were treated intragastrically by gavage with ST 3000?μm/kg.The mice were killed 1 hour,2 hours,4 hours,8 hours,16 hours after oral administration respectively for observing ependymal cells,neurons and endothelial cells of blood vessel of the lateral recess with transmission electron microscopy.

BALB/c小鼠，ST单次灌胃，分别于灌胃后1、2、4、8、16 h处死动物，用透射电镜观察外侧隐窝室管膜细胞、神经元、神经纤维和血管内皮细胞的结构变化。

AIM: From the views of biochemistry, histology and molecular biology, this study observed the effects of 4-6 Hz vibration on concentration of hexosamine, the expression of mucin 5AC mRNA in gastric mucosa and histomorphology, and investigated the effects of body vibration on rats' gastric mucus secretion and mucosal structure.

邓 艳，陈伟华，宋小丹，张源淑南京农业大学动物医学院，农业部生理生化重点开放实验室，江苏省南京市 210095目的：从生物化学、组织学和分子生物学角度观察4~6 Hz振动对大鼠胃黏膜氨基己糖含量、黏蛋白5AC mRNA表达及其组织形态学变化，探讨振动对大鼠胃黏膜黏液分泌及形态的影响。

Observe the effect of Fuling Gancao Decoction on gastric liquid emptying and content of nitric oxide and acetylcholine esteras in the gastric fundus,antrum and duodenal tissu of functional dyspepsia rats and investigate the mechanism.1 Reference reviewThe reference review discussed the source of disease name of TCM for FD,etiology and pathogenesis of TCM,selection of therapeutic methods by different stages or according to the differential syndrome diagnosis,advance on the clinical and experimental research as well as the problems lying in the research of TCM and corresponding strategies.We also have summarized the recent research progresses on the FD.In the meanwhile,we further exploited the pathologic characteristics,diagnosis and treatment of FD;summarized etiology and pathogenesis of TCM for FD and application of Classical Prescriptions in clinical and experimental study;Collected and arranged the Literatures and summarized the recent research progresses of Fuling Gancao Decoction;Finally, collected and arranged the literatures of proved cases and clinical experiences of Fuling Gancao Decoction.2 Theoretical studyThe Theoretical study first discussed the source of TCM for stomach deficiency and congestion of fluid-retention in syndromes of FD;Secondly, summarized the indications of Fuling Gancao Decoction,that is:①epigastric throb,②cold hands and feet in syncope,③hydroadipsia,④gastric blockade,⑤gastric splashing sound,⑥no desire for drinks,⑦light white tongue,thin-white and smooth fur,⑧deep and wiry pulse are the indications of Fuling Gancao Decoction;Subsequently,analyzed the composition of Fuling Gancao Decoction,considered that ginger is the main drug which treating congestion of fluid-retention in Middle-Jiao by Zhang Zhongjing.3 Experimental study3.1 Experiment oneObjective:To establish the animal model of FD with delayed gastric liquid emptying and evaluat it.

同时观察茯苓甘草汤对FD大鼠胃液体排空及胃底、胃窦及十二指肠中乙酰胆碱酯酶、一氧化氮含量的影响，探讨茯苓甘草汤的作用机制。1文献综述参照古、今中医有关文献对FD的中医病名、病因病机、辨证论治方法、临床及实验研究进展进行了探讨，并对现代医学在FD研究中所取得的(来源：A0bBC论文网www.abclunwen.com)进展进行了总结，进一步明确了FD的发病机制、诊断和治疗的研究进展；总结了中医对FD的病因及发病机制、中医诊断及经方治疗FD临床及实验研究进展；并搜集和整理已发表的有关茯苓甘草汤研究报道，阐述了茯苓甘草汤的现代研究进展；最后搜集和整理了近现代著名医家应用茯苓甘草汤的经验及验案。2理论研究理论研究部分首先对功能性消化不良胃虚饮停证的认识进行了理论溯源；其次对茯苓甘草汤的适宜证象进行总结，认为：①心下悸，②四肢不温，③口不渴，④胃中痞满，⑤胃中振水音，⑥不欲饮水，⑦舌淡白，苔薄白而滑，⑧脉沉弦是茯苓甘草汤的主证；随后对茯苓甘草汤方药组成解析，认为生姜是仲景治疗中焦饮停的一味主药。3实验研究3.1实验一目的：探讨建立功能性消化不良胃虚饮停证的动物模型方法并对其进行评价。

Regular diet, in addition to containing a large number of animals necessary nutrients, but also contains a certain amount of anti-nutritional factors, these anti-nutritional factors is difficult to digest in the use of sheet monogastric animals, so that intestinal contents became viscous, and wrapped a large number of nutrients, affect the digestion and absorption of nutrients, resulting in animals indigestion, lax and poor growth.

常规日粮中，除含有大量动物必须的营养物质外，还含有一定量的抗营养因子，这些抗营养因子难被单胃动物消化利用，使肠内容物变得粘稠，而且包裹大量的营养物质，影响营养物质的消化吸收，造成动物消化不良，排粘粪，发育不良等。

The mouse was carried out half nephric duct ligation to induce the renal interstitial fibrosis animal model,afterwards,to set up three groups,control group,sham operation group(intragastric administration with normal sodium according to 10ml/kg weight),treatment group...

采用小鼠单侧输尿管结扎法诱导肾间质纤维化的动物模型，用盐酸青藤碱治疗，并设对照组、假手术组(小鼠每日按10m l/kg体重用生理盐水灌胃，直至治疗结束)、治疗组(选用正清风痛宁缓释片按每日30mg/kg体重给小鼠灌胃，连续3周，)，检测血尿素氮、血肌酐、血清层粘连蛋白、纤维连接蛋白的含量，免疫组化法测肾脏组织转化因子β1(TNF-β1)的表达并进行肾脏病理学的观察。

The extract of Raphanus sativus roots demonstratsd stimulatory activity on gastrointestinal tract. And this activity may be induced by the increase of motilin content and enhancement of gastrointestinal electric activity.

白萝卜提取物可明显促进实验动物胃肠运动，该作用的发挥可能与胃动素水平升高及促进胃十二指肠电活动有关。

Experimemt 2: The nylon bag technique was used to study degradability of dry matter and protein and degrading characteristics of AM and PLM in the rumen of three goats by fixing perpetual rumen cannulae, and the degradability difference between the two roughages was analyzed.

试验二：以3头安装永久性瘤胃瘘管的关中奶山羊为试验动物，用尼龙袋技术研究了苜蓿草粉和柠条叶粉蛋白质和干物质的瘤胃降解率及降解规律，分析了两种饲料原料降解率的差异性。

Following sacrifice, some vomicas containing yellow and white liquor puris on the chest wall of 4 pigs were observed, which were wrapped but did not communicate the thoracic cavity. One artificial esophagus was not in place but found in the stomach. One artificial esophagus was twisted and formed an esophagus diverticulum. The false passage around the prosthesis formed so long as the animals survived more than 1 week, which was called as neo-esophagus.

动物处死后，从原胸壁切口开始进胸，4只动物见胸壁存在小脓腔，腔内见黄白色脓液，已包裹，与胸腔不相通，1只动物人工食管脱落后滞留于胃内；1只动物因术中吻合时缝合的人工食管过多且边距不均匀，导致人工食管扭曲，形成食管内嵌。1周时间人工食管周围形成假道，即&新生食管&，包绕人工食管。②

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。