动物实验的

And AEV agar gel precipitin antigen and an inactivated oil-emulsion vaccine against AE were produced. The purpose of this study, on the base of former research, was to reveal and better understand some molecular pathogenesis of AE, to set up a working model which could be generally used by anther researcher in the field of animal molecular pathology.

本项实验研究，旨在过去工作的基础上，利用分子生物学、分子免疫学的技术手段，探讨和揭示AE发生发展过程中的某些分子发病学机理的问题，并试图建立一种具有普遍指导意义的动物分子病理学研究的工作模型。

It was proved that the method which wasonvenient, rapid and reduplicate could be used to identify different samples of Oviductus Ranae. We would establish a sensitive and specific HPLC method for the quantity control of Oviductus Ranae.

本实验不仅建立了哈蟆油的HPLC指纹图谱，而且还首次成功地利用该方法对哈蟆油的真伪进行了鉴别，能够有效的监测哈蟆油的质量，作为其质量控制的手段，为名贵动物药哈蟆油的开发和利用奠定了基础。

By observing the effect of the short-distance transportation stress on the experimental animals, the method establishes the short-distance transportation stress evaluation, comprises technology index systems such as energy metabolism totem GLU, immunologic function executor WBC, HPA axial final effect laser CORT, etc., ensures that the health adaptive stage after the short-distance transportation stress is at least 72 hr and provides the nutrition replenisher intervention measure for relieving the adverse reaction of the short-distance transportation stress.

本发明通过观察短途运输应激对实验动物的影响，建立了短途运输应激评价的包括：能量代谢标志物GLU、免疫功能执行者WBC、HPA轴终末效应激素CORT等的技术指标体系，确定了短途运输应激后的健康适应期至少为72hr，并提供了减轻短途运输应激不良反应的营养补充剂干预措施。

Objective: The research was used the methods that had been improved by our lab for miRNA clone and expression analysis. Oreochromis nilotica, Xenopus laevis, Trachemys scripta elegans, Gallus gallus four species from Pisces、Ammphibia、Reptilia、Ayes had been used as the meaterrl to fred out the impacts of miRNA on animal evolution in this research as well as rich the miRNA database.

目的：利用本实验室改良的miRNA克隆方法及检测方法，我们从鱼纲、两栖纲、爬行纲、鸟纲中分别选取罗非鱼、蟾蜍、巴西龟、家鸡四种动物的脑为实验材料，丰富物种的miRNA数据库，并初步探讨miRNA在物种进化中的作用，为研究物种的进化提供新的思路和方法。

Methods: Adult mice of Kunming variety were used as experiment animals. After judgement of oestrus, the oestrus period and oestrus stage were determined, and then those mouse were grouped depending on oestrus stage. The mice were dealed with diferrent dosage of PMSG and HCG to make them superovulation, afterwords were mated individually with the male mouse together at same cage. The embryoes were rinsed out and evaluated after 3.5 days when the plug had been seen. The transplant mice were born after the high-quality embryoes were transferred into the receptors mouse uterus at the same oestrus stage which was stimulated by mating with the male mouse which spermaduct had been ligated previously.

以昆明系小白鼠为实验动物，取性成熟个体，先进行发情鉴定，确定其发情周期及不同的阶段，然后按发情阶段分组，分别用不同剂量的孕马血清促性腺激素和人绒毛膜促性腺激素进行超排处理并与公鼠合笼，在见栓后的第3.5天冲胚，对所得胚胎进行鉴定；将质量较好的胚胎选出，移入同期发情并经结扎公鼠交配刺激后见栓的受体小鼠子宫内，得到移植小鼠。

NTC, which holds many prestigious scientists whose names spread throughout the world, high-efficient scientific faculty, advanced equipments, top-rank laboratories as well as three high-grade experimental animals bases, equipped with humanized scientific supervision, are making great strides.

该机构拥有国内外知名的科学家，高效率的科研队伍，先进的仪器设备，一流的实验室及三个高品质的实验动物基地，并配以人性化的科研管理，正以惊人的速度发展壮大。

And unrooted trees were established. The accession numbers in GenBank of the sequences of 16S rDNA of the strains were followed by PFK1(DQ295034), PFK2(DQ295035), PFK3(DQ295036), LZCL(DQ295040), ZCS1(DQ295037), MMA1(DQ295041), MMA2(DQ295038), JSQ1(DQ295042), JSQ2(DQ295039), JSQ3(DQ295043). The Blast and phylogenetic tree analysis showed that

3将分离菌株进行形态、生理和糖发酵实验，结果表明这些表型鉴定的结论与16 S rRNA基因序列同源性分析结论一致，分离纯化5种产品的10株菌中仍有相同的3株菌与标签标注的不一致，即其中标签上分别标注为青春双歧杆菌和长双歧杆菌的两株菌鉴定结果均为动物双歧杆菌

SLE is involved in immediate allergic II~IV types reactions. The overexpression of immediate allergic reactions can be the potential factor to induce cell apoptosis. In the past researches, cystamine which is the inhibitor of TG2 and caspase can slow down the expression of SLE through suppressing cell apoptosis and also can prolong the ratio of surviving in NZB/W F1 mice model in serological and protein test.

其广泛的牵涉了II~IV型的过敏反应，过敏反应的过分激活为自体抗体大量产生引发细胞apoptosis的潜在因素，在本实验室过去曾发现在NZB/W F1 mice作为实验动物模式之下，以cystamine此TG2及caspase的抑制剂具有在血清学及蛋白检测中均可透过抑制细胞的凋亡，进而减缓SLE达到保护的作用，改善狼疮病症，且延长其存活率。

In this research, the pubertal five-week-old SD rats were chose as experimental object to establish the animal model for simulating maxillary protraction, on which was imposed the orthopedic force of 85g with utilizing the self-devised maxillary protraction appliance. Located lateral X-ray cephalograms were taken to measure and analyze the changes of maxillary development after exerting the orthopedic force for 4 weeks. The technique of immunohistochemistry was used to investigate theexpression of TGF-, in frontomaxillary sutures and palatomaxillary sutures with identical force performing for different extent of time. The average hue was selected as indicator, which was measured by the means of the analysis system of pathological color images. The measuring data were analyzed by the one-way ANOVA in software SPSS 11.0 version.

本研究采用自行设计的上颌前牵引装置，以生长发育期5周龄SD大鼠为实验对象，施加85g的矫形力，建立了模拟上颌前牵引的动物模型，通过X线头影测量分析，观察施力4周后上颌骨的生长发育情况；应用免疫组织化学技术，检测同一力值作用不同时段下TGF-β_1在上颌骨的额颌缝、腭颌缝的表达，彩色病理图象分析系统测定每张切片中阳性染色细胞的平均灰度值，结果采用SPSS 11.0进行方差分析。

Methods Twelve dogs were randomly divided into experimental and control groups (n=6). They were treated with two kinds of thoracic cavity closed drainage system respectively after artificial hemopneumothorax. The time of thoracic cavity closed drainage, anti-tensile strength,and hemoglobin contents in peripheral venous blood before operation and after reinfusion were observed.

对比分析自行研制的新型多功能胸腔闭式引流装置与现有的胸腔闭式引流装置对实验犬（n=6）及对照犬(n=6)的多项指标的影响，如胸腔闭式引流术所用时间、两种装置的抗拉力、引流胸血及胸血回输情况和创伤前及回输后周围静脉血液血红蛋白含量的变化以及动物生存情况等。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。