动物实验的

Now animals studies and test tube experiments are beginning to show that certain anaesthetics reduce the rate at which brain cells are born and develop, a factor that seems to be important to normal memory function.

目前动物研究和试管实验中已开始显示某些麻醉药减少的速度在脑细胞的诞生和发展的一个因素，这似乎是重要的，以正常的记忆功能。

The genome DNA was extracted from the red blood cells or blood of the infected sheep and goats which include as isolates of ovine Babesia from these different geographic areas. The gene specific primers were devised according to the pubulished 18srRNA sequence at GenBank. The target DNA segment was amplified by polymerase chain reaction, the PCR product was ligated into PGEM-Teasy vector.

用分离于四个地区的羊的巴贝斯虫感染实验动物，自感染羊的红细胞或全血中提取基因组DNA，根据GenBank中已发表的巴贝斯虫18S rRNA序列设计两对羊巴贝斯虫特异性引物，通过PCR扩增目的基因片段，将扩增产物连接到PGEM-Teasy载体中，经酶切鉴定，PCR分析，进行序列测定，结果显示羊巴贝斯虫四个分离株的18S rRNA基因大小为1800bp左右。

And 37. 1%, respectively. The results suggest that 18α-GA down-regulated CYP expression at the transcriptive levels.

表明GL在转录水平下调大鼠肝细胞CYP1A1，CYP2E1和3A1表达，以对CYP2E1的抑制作用最强，对CYP3A的抑制作用最弱，对CYP1A1的抑制作用居中，与整体动物的酶学实验结果基本一致。

And try to find out the effect of TSA on the proliferation and differentiation of rat tracheal stem cell. Methods 1.Preparation of 5-Fu Model and TSA treatment Two weeks" Wistar rats provided by the animal center of the China Medical University. The animals were sacrificed by 10% Chloral Hydrate 0.4ml/100g. Then the tracheas were excised aseptically. Then they were douched with PBS and cultured in 1:1 mix of Dulbecco"s modified Eagle"s medium and Ham"s F-12 medium (DMEM/F12) containing 120 mg/ml 5-Fu and 10% fetal bovine serum for 12h at 37°C. The tissues were then refreshed by culturing in DMEM/F12 containing 10% FBS and 200nM TSA.

1、制备气管损伤模型及剥离上皮取约200克左右的Wistar大鼠，雌雄不限(中国医科大学实验动物中心提供)，腹腔注射10%水合氯醛0.4ml/100g，无菌条件下取出气管，无菌PBS反复冲洗，洗净血液和粘液，置于DMEM/F12培养液中(含10%胎牛血清)，于培养液中加入终浓度为100mg/ml的5-Fu，37℃，5%CO2孵育12小时，弃去上述培养液，换成新鲜DMEM/F12液(含10%胎牛血清)，同时加入终浓度为200nM的TSA继续培养，于换液后3、6、12、24、48小时分别10%中性福尔马林固定，石蜡包埋，制成4μm厚的组织切片。

This method of developing model economized the quantity of elastase and increased the survival rate of animal in hypoxic condition than our previous experiments.

本模型的建立方法较本组以往的实验方法既节省了弹性蛋白酶的用量，又提高了缺氧动物的成活率。

Through reviewing the reproductive toxicity of some major environmental chemical pollutants, including endocrine disrupters, organic phosphor and chlorine pesticides, organic solvents, and some metals such as lead, cadmium, mercury, manganese, the paper finds that all these ECPs have different extents of toxic effects to experimental auimals on procreation, gestation and even on offspring, some of which have been evidenced by the population epidemiological studies.

本文通过对邻苯二甲酸酯类和双酚A等内分泌干扰物、有机磷和有机氯农药、常用有机溶剂以及常见金属的生殖毒性研究的综述，发现它们均在不同程度上造成对实验动物的生殖毒性、妊娠毒性以及对子代的毒性影响，其中部分在人群流行病学研究上得到证实。

The purpose of this reviewis to present a structured assessment of the anti-tumour and cytotoxic properties of 150 marine natural products, many of which are novel compounds that belong to diverse structural classes, including polyketides, terpenes, steroids and peptides.

产生这些活性化合物的生物包括无脊椎动物、海藻、真菌和细菌。在大量的实验及临床模型上，研究人员对31个结构明确的海洋天然产物进行了抗肿瘤药理研究，这样的研究进一步明确了其作用机制。

In vivo experiments showed that this kind of novel vaccine could not only protect mice from syngeneic and allogeneic tumor challenge but inhibit the growth of established tumors.

接着进行的动物体内实验证实这种新型疫苗不仅能够保护小鼠免受同系以及不同系肿瘤细胞的攻击，而且对小鼠已形成的肿瘤亦有明显的治疗作用。

Unlike Pavlov's dogs or other animals who respond only by instinct, we humans have the power of choice.

不像仅靠本能作出反应的巴甫洛夫（通过狗的实验研究了条件发射）的狗或其它动物，我们人类拥有选择的能力。

The cryobiology has been applied for breeding, preservation of genetic resources, and development of new strains of laboratory animals.

随着低温生物学理论的不断深入及其技术的不断完善，低温生物学技术已被广泛应用于实验动物的生产、遗传资源的保存及新品系开发等领域。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。