动物实验的

To establish a series of monoclonal antibodies for specific gene products of H-2 complex will not only provide probes for the research on gene constitution of H-2 complex, but also will provide effective genetic quality control measures for the international standardization of laboratory animals in China. It also can offer essential conditions for sophisticated research works in life science field, such as immunogenetics, organ transplantation, etc.

建立一系列H-2特异性基因产物的单克隆抗体，能为研究实验小鼠H-2复合体基因组成提供探针，为我国实验动物的国际标准化提供有效的遗传质量控制手段，也能为免疫遗传学、器官移植等生命科学领域内的尖端研究提供实验室工作方面的重要条件。

Results: diazepam increases the effect of AA.picroloxin and isoniazid reduce it and antagonize the analgesia effect of ACTH; diazepam increases the content of 5-HT in hipocampus,hypothalamus and midbrain;diazepam + ACTH increases,more than AC-TH or diazepam singly,the level of 5-HT in the 3 brain regions; picrotoxin and isoniazid reverse the effect of diazepam and ACTH on the content of 5-HT in the 3 brainsites; potentiation of diazepam on AA is antagonized by naloxone and atropine.

本实验用腹腔注射干扰GABA代谢和影响其受体药物的方法，探讨GABA在ACTH镇痛中的作用并初步观察其可能的机理。材料与方法实验用体重30～50g左右雄性小鼠153只。一，痛闽测定将动物置入改良的特制的铁笼内¨1f12，以恒速增长的电流强度刺激鼠足跖部

But whether the compound composed of them has suppressive effect on absence - like seizures of genetic tremor rats hasnt been reported. Furthermore, the animal models of investigating antiepileptic drugs were mostly induced by chemoconvulant or electrical stimulation. Since genetic factor is more primary in clinical epileptic patients, we used tremor rats fetched in from Japan as experimental animal models. We implanted electrodes in cortical and hippocampus of tremor rats and then recorded the electroencephalograms to observe effects of the traditional Chinese medicinal compound composed of Scolopendra, Bombyx Batryticatus, Uncaria and Arisacma Consanguineum on absence - like seizures in tremor rats.

而且以往多以化学致痫剂或电刺激等方法造模进行抗癫痫药物的研究，但在临床的癫痫患者中遗传因素占有相当大的比例，所以本实验应用了从日本引进的具有遗传性癫痫小发作的震颤大鼠作为实验动物模型，通过在震颤大鼠脑皮质和海马慢性安插电极后进行脑电图的记录，观察以蜈蚣、僵蚕、钩藤、制南星四味药为主的复方中药制剂对震颤大鼠癫痫小发作的抑制作用。

The method makes model of small rat gastric ulcer, go alone with the home stomach of fill of colza leach fluid gives drug, observation test group and contrast the cankerous index of mucous membrane of group of small rat stomach, discuss its to advance a law to observe the home goes alone with charcoal end to protective action; of the stomach the influence that colza water steep moves to small intestine passes experiment leaving put oneself in another's position to observe its the action; of flowing to small rat small intestine flesh observes with heating up board law the home goes alone colza water.

方法制作小鼠胃溃疡模型，以家独行菜子水浸液灌胃给药，观察实验组和对照组小鼠胃黏膜的溃疡指数，探索其对胃的保护功能；以炭末推进法观察家独行菜子水浸液对小肠运动的影响并通过离体实验观察其对小鼠小肠平滑肌的功能；以热板法观察家独行菜子水浸液对实验动物的镇痛功能。

Objective To study the protective action of compound agent madeup with effective ingredients of Hirudo and Rhizoma chuanxiong and Folium Ginkgo in animal ischemic cerebral infarction.Methods To mix Hirudin extracts of Folium Ginkgo and Ligustrazine according2:25:5into asepsis injection,rats local cereˉbral ischemia induced by embolism of middle cerebral arterywas used as experimental model to study the efˉfects of the compound agent and positive control drug,Nimodipine and extracts of Folium Ginkgo on cerebral infarct size and neurological deficits.Disposing the experimental data with statisticsand comparing their otherness among difˉferent groups.

目的 探讨用中药水蛭、银杏、川芎的有效成分组成的复方制剂对动物局灶脑缺血的保护作用方法将水蛭素、银杏叶提取物、川芎嗪按2∶25∶5的比例制成无菌注射液，应用鼠大脑中动脉栓塞导致缺血性脑卒中的实验模型，并用生理盐水、尼莫地平和银杏提取物作对照，观察不同药物对脑缺血面积和由缺血造成的大鼠行为障碍的影响，将实验据用统计学处理并进行组间的差异性比较。

Methods After the solution made from artificial grown lumbricus was sprung to the wound of animal and patient after hemorroidectomy, the wound inflammation, wound healing and changes of laboratory determinations were observed and compared to those of the control group.

将人工养殖的鲜地龙制成的溶液喷洒于实验动物及混合痔手术后患者的创面，观察其创面的炎症情况、愈合情况以及各实验观测指标的变化，并与对照组进行比较。

The experiment on electromagnetism pulse biology effect could suggest that quantities-effect of EMP effect on system morphemic and function with applying different frequency, power and expose time. Combined with epidemic investigation and medical psychological research in EMP researcher, the immediate, early stage and middle and long-term biological effect of EMP was elucidated and EMP protection standard was established.

电磁脉冲(electromagnetism pulse，EMP)生物学效应实验是通过应用不同频率、功率、暴露时间的EMP照射动物，明确EMP所致各系统形态和功能危害的量效关系，探索其效应机理；并结合对EMP从业人员的流行病学调查和医学心理学研究，揭示EMP所引起的即刻、早期、中期和远期生物学效应，提出EMP卫生防护标准，为物理防护和医学防护的研究提供重要的理论依据和实验依据。

Much early work on learning was actually done on rats and other animals (and much of this research was unjustifiably cruel, but that is another matter).

过去的人们为了研究学习过程这个问题实际上已经在老鼠和其他动物身上做过很多实验（而且很多的这些实验对于都是极端的残忍，当然，这就我们的主题无关的另一方面问题）古典制约作用。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。