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During secondary inner ear immune responses against keyhole limpet hemocyanin,to understand the change of interleukin-6 (IL-6) levels in perilymph and serum, IL-6 levels in perilymph and serum were investigated using an immunoassay.

为了解在用keyhole limpet hemocyanin引发的大鼠继发性内耳免疫反应时,实验动物血清及外淋巴液中是否有细胞因子活性的变化,采用免疫测定方法对实验动物血清及外淋巴液中白细胞介素(interleukin-6,IL-6)活性进行测定。

In animal, up to the present, there are a few reports about lipoid pneumonia, especially, in laboratory animal, there is no report regarding lipoid pneumonia induced by endogenous factor.

有关实验动物的类脂质性肺炎,在世界上少有报告,尤其实验动物由内因性引起的类脂质性肺炎,在世界上未见有病例报告。

"Vivisection: Operation on a living animal for experimental rather than healing purposes; more broadly , all experimentation on live animals."

活体解剖:为了实验而对活的动物做手术,目的不是为了治疗;更广义来讲,对活的动物所作的所有实验。

Animal and cell line: One depuratory grade Kunming Mus musculus albus pregnant for 12.5 days, was provided by the Nanchang University Medical Animal Center.

动物及细胞系:清洁级孕12.5 d昆明小白鼠1只,由南昌大学医学院动物中心提供,实验过程中对动物的处置符合动物伦理学标准。

The mice inoculated with Coxiella burnetii intrana- sally developed interstitial pneumonia,while the primary pathological changes of mice inoculated intraperitoneally are granulomas in spleen and liver.2.The pathological changes became more severe followed the dosage increasing.3.Coxiella burnetii can be detected in spleen and liver at day 2 after inoculation.the lesion became more and more serious from day 2 to day 12.The characteristic changes were observed at day 7,and recovered at day 14. 4.The reticuloendothelial system are main target of Coxiella burnetii.The pathogen was detected in cytoplasm of monocyte -macrophages of spleen, liver, lung, and endothelioid cells of blood vessel. 5. Coxiella burnetii can be found in macrophages lysosomes by electron microscopy. Most of them are round or rod, and polymorphic shape can also be observed in different size.

结果:1、通过不同感染途径的实验证实,滴鼻感染的小鼠主要表现为间质性肺炎,而腹腔注射感染小鼠则以脾脏、肝脏肉芽肿为主要病变。2、通过不同剂量的感染实验发现,随着感染Q热立克次体剂量的加大,动物病变愈加严重。3、通过感染后不同时间的动态病理学观察发现,在腹腔注射后第2d的脾和肝脏即可发现病原体,主要脏器的病理变化从第2d到第12d逐渐加重,第7d动物的病变最典型,至感染后14d动物的受损器官已开始出现修复性变化。4、 Q热立克次体主要侵害机体的网状内皮系统,在感染小鼠的肝、脾、肺和外周血管单核巨噬细胞以及血管内皮细胞胞浆中查见病原体。5、透射电镜观察可见Q热立克次体主要位于巨噬细胞吞噬溶酶体内,呈多形性,多见圆形和杆状,大小不一。

Methods We developed hepatic failure by two methods.(1) Surgical moldel of hepatic failure: Under general anesthesia, an end-to-side portocaval shunt was performed in seven trial minipigs. Forty-eight hours later after operation, these minipigs underwent transient total liver ischemia for 4 hours by ligating the hepatoduodenal ligament.

应用中国实验小型猪7头,采用Ⅰ期门静脉-下腔静脉吻合、Ⅱ期(48h后)供肝动脉暂时结扎4h的方法建立缺血性急性肝功能衰竭动物模型;中国实验小型猪6头给于1.2g/kg的D-氨基半乳糖诱导肝功能衰竭,建立药物性急性肝功能衰竭动物模型。

This experiment will discuss the method to create high lipohemia model of rabbit to observe the effect of Bijia Mountain brand mineral water on the level of the blood lipid of the animals with lipohemia.

本实验摸索实验动物家兔高脂血症模型造型的方法,观察笔架山牌矿泉对模型动物高脂血症的影响。

Assessment of animal behavioral effects is widely used in researches of central nervous functions , such as motorial function , learning and memory .

实验动物行为分析广泛应用于运动功能和学习,记忆等高级中枢神经功能的研究,较电生理,生化等其它参数分析方法,具有全面反应实验动物整体状态的特点。

Methods Scarfskins sheared from four different animal's skin were made into specimens, and then treated with α-MSH under the technique of immunohistochemical staining.

方法]对4组不同正常实验动物的表皮标本进行组织切片,应用免疫组织化学技术,观察α-MSH在4组不同实验动物表皮中的表达。

Many of the experiments were carried out with animals (in the famous'Skinner Box') and the first difficulty concerns the transferability offindings with animals to complex human behaviour, of which language isa prime example.

由于许多实验都是在动物身上进行的(例如著名的斯金纳箱实验),最先遇到的问题就是如何将从动物身上获得的实验结果转换到复杂的以语言为范例的人类行为上。

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