动物实验的

Medical colleges were instructed that if they wished to benefit from the Rockefeller largesse they had better convince 500 million Chinese to throw into the ashcan the safe and useful but inexpensive herbal remedies of their barefoot doctors, which had withstood the test of centuries, in favor of the expensive carcinogenic and teratogenic "miracle" drugs Made in USA, which had to be replaced constantly with new ones, when the fatal side-effects could no longer be concealed; and if they couldn't "demonstrate" through large-scale animal experiments the effectiveness of their ancient acupuncture, this could not be recognized as having any "scientific value".

医学院校被告知，如果它们想从洛克菲勒慷慨的赠与中得到好处，它们必须使五万万中国人民信服地把他们经过多少个世纪检验的赤脚医生安全、有效却又廉价的草药扔到垃圾箱里，让中国人民赞成使用美国制造的昂贵的有致癌、致畸作用的"神"药，当这些药致命的副作用再也掩盖不住的时候，则需要不断地用新药来替代；如果他们不能通过大规模的动物实验来"验证"他们古老的针灸的有效性，这就不能认为有任何"科学价值"。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Thus gp130 is the common signal transduction molecule. Gp130 molecule expresses universally on many tissues including immune system, hemapoietic system, and nerve system. Gp130 has an essential role in the function and regulation of the above systems, especially in the development of hemapoietic system. The signal transduced by gp130 is necessary during the differentiation and proliferation of hemapoietic cells. It is evidenced that the embryo of gp130 gene-knockout mouse was dead due to a serious impediment of the development of hemapoietic system.

Gp130在人体组织细胞上广泛性表达，介导和调节机体的免疫、造血以及神经系统的生理功能，尤其是在造血系统的发育过程中，具有极其重要的作用，动物实验表明，敲除小鼠的gp130分子基因可以使其在胚胎的早期阶段产生严重的造血系统障碍，最终导致流产，由此表明在造血细胞的分化、发育和生物学行为中，gp130介导的信号是必不可少的。

The dopamine release in vivo was monitored by fast cyclic voltammetry and the concentrations of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic acid were determined by high performance liquid chromatography electrochemical detection in the process study. In addition, the effects of some traditional Chinese herbs and related marine drug on behavioral changes were also evaluated in order to provide some suggestive clue for rational drug design of anti-Parkinon's disease .

许多因素都参与PD的DA能神经元的死亡，所以，本研究在制备PD动物模型的基础上，应用国内率先引进的快速周期伏安法检测设备在体动态监测DA的释放，同时应用另一种电化学检测技术—高效液相色谱电化学检测法离体检测DA及其代谢产物二羟苯乙酸和高香草酸的含量，结合行为学测定及其它方法，研究了雌激素和铁在PD发病中的作用，以及一些药物，如吲哚醌、牛磺酸、铁离子螯合剂、胆囊收缩素（CCK-8）、乌鸡白凤丸有效成份、大豆异黄酮及海洋酸性寡糖HSH971，对DA能神经元的影响，从DA能神经递质角度对PD的发病机理提供进一步的实验依据，并为PD的预防和治疗提供新的干预措施。

Later, under the untypical parasternal four-chamber view which can thoroughly display the ostium of coronary sinus, the catheter was promoted further to the ostium of coronary venous sinus. Then the echocardiography technician adjusted the transducer of TTE in order to thoroughly confirm the position of the catheter tip. After confirming the position of catheter tip in the coronary venous sinus, the operator inserted the catheter into the coronary venous sinus for 3～4 mm farther. It was noted that if resistance was encountered by operator, the operation must be stopped, which was the same as x-ray fluroscopy as image guiding. TTE guiding His bundle elactric cahteter、high right atrium electric cahteter and right ventricular electric catheter in site: It is difficult using TTE singly since there are too many crossroads in inferior venous.

本研究结果显示，(1)单独应用TTE作为影像学引导进行冠状窦电极导管置放的成功率为93.8％～96.7％，并且应用TTE作为影像学引导放置专用冠状窦电极导管和普通电极导管的成功率和放置时间在统计学上无显著差异；(2)TTE作为影像学引导，除过度肥胖患者外，可完全替代X线引导进行冠状窦电极导管置放，包括鞘管和扩张管的放置、指引导丝在静脉而非动脉内的证实均可由TTE完成引导，且较X线C型臂有一定优越性；(3)TTE结合普通X线胃肠透视机作为影像学导引，可顺利完成心内电生理检查时导管的安全到位，而不需昂贵的X线C型臂，可将心内电生理检查扩大到广大的基层医院；(4)TTE较X线更易和更早期发现介入治疗的并发症如急性心包填塞等，对并发症的防治甚至挽救病人的生命至为重要；(5)动物实验研究表明，TEE可引导射频导管消融术时大头电极导管成功到位。

However, there were few reports on the changes in perioral muscle pressures following RME treatment and retention and none domestically.In this study, a new perioral muscle measuring system was invented to measure the values and changes in cheek and tongue pressures before, after treatment and retention in order to supply experiment foundation and theoretical guiding for clinical research and treatment.The study consisted of two parts as follows:In the first part, we invented a new perioral muscle measuring system.

此后学者们进行了广泛的动物实验和临床观察，其中RME的治疗及保持是临床医生非常关注的问题，但RME引起的口周力变化以及这种变化与RME治疗和保持的关系鲜有报道，国内尚未开展此项研究。

125I seeds interstitial brachytherapy is a new kind of method to treat with malignant tumors. The aim of the experimental research through animals is to investigate the therapeutic effect and the pathological changes on rabbits' VX_2 liver tumor treated with ~(125)I seeds interstitial brachytherapy and percutaneous ethanol injection under CT guidance. We aim to identify the therapeutic effect and the criterion of ~(125)I seeds radiant activity selection, optimize the therapeutic regimen, investigate the tissue damage, its' prothetic time and the relationship between time and dosage effect, and approach to provide fundamental theories for ~(125)I seeds clinical using and arrive at the best dosage range of ~(125)I seeds interstitial brachytherapy. And it can become a new and effective kind of therapies to be applied for the clinical treatment to mid-advanced stage and recurring liver tumor.

放射性粒子组织间植入是肿瘤治疗的一种新方法，本研究通过动物实验探讨CT导引下瘤体内~(125)I粒子植入治疗，以及~(125)I粒子组织间植入联合经皮乙醇注射综合治疗兔VX_2肝转移癌的疗效及其病理基础，确证~(125)I粒子组织间植入治疗肝癌的有效性，确定治疗高度恶性肿瘤的粒子选用标准和最佳治疗方案，同时探索~(125)I粒子在家兔肝脏组织间植入内照射后放射损伤和修复的变化及其时间与剂量效应的关系，提出临床应用~(125)I粒子组织间植入治疗的最佳剂量范围，为该疗法在实际应用中合理化治疗方案的取得提供理论依据，并为临床中晚期及复发肝癌的治疗提供一种新颖、有效的手段。

These two peptides can help functional proteins rapidly translocate into cells both in vitro and in vivo in a non-invasive manner, and preserve their biological actions in several cell and animal models.

这两个胜肽链在试管与活体可以以不具侵略性( non-invasive )的方式携带开展型态的功能性蛋白质快速的进入细胞内，并且在许多的细胞与动物实验中均可以保留它们的生物功能。

MethodsThe activity of macrophage cell in abdomen cavity and NK cell in spleen and the SIgA contents in saliva were detected, and the survival rate of mice which had been infected by influenza virus FM 1 were observed after the powder of Le IFN α/supplement material mixture had been insufflated in mice buccal cavity.

一些研究表明，人体的干扰素受体，主要分布在口腔、腭咽及鼻腔黏膜部，只要少量的IFN同上述部位的受体结合，即可发挥IFN的生物活性[1] ，同时也有不少以人的αIFN通过口腔途径给药用于抗病毒和免疫调节的动物实验[2 ] 。

Previously, short echo time STEAM sequence and short TE PRESS sequence have been used to quantify brain Cr concentration.

综上所述，本研究旨在通过动物实验，以长TE的PRESS序列外标准方法及LCModel软件定量检测活体小猪的脑Cr浓度，然后用高效液相色谱方法分析相应离体脑匀浆液的Cr浓度，比较两种方法检测结果的相关性，以此探讨活体~1H-MRS方法检测结果的准确性。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。