动物实验的

Experimental animals were allowed to swim with different loads in a water pool at constant temperature.

结果 研制出一台可同时做六路平行实验的动物游泳测试仪，自动记忆动物在加载的情况下抗疲劳游泳的全过程。

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

Establish a quantitative detection method in micro level of blood on the base of reagent box for detecting the TC, TG, and HDL-C in ordinary amount of blood .

为能够用小鼠做实验材料进行降血脂保健食品的动物实验研究，建立了用微量血测定血液中TC、TG和HDL-C的方法。

And it was divided into four groups at randomly (n=6),The vertical compression,bending and torsion intensity in without and with reduction of cortical fragment of plate fixated contra side, without and with reduction of cortical fragment of plate fixated sidewere measured.A11 results were analyzed with statistic method.2、animal experiment :The 12 adult goats were divided into two group at randomly,wedged cortical defect with 1 cm of long and conjugate diameter was created in the middle-part of the medial femur and plate was fixed in the defect side and as the experimental side,the other side was control.after operation 4、8、12 weeks,investigation of the fracture healing by means of gross observation,X-ray examination and histology in both sides.

测试各组的垂直压缩、三点弯曲及抗扭转的刚度。2、动物实验：12只健康成年山羊，雌雄不限，体重在20～25kg之间，在同一只羊双侧股骨中段内侧制造楔形骨皮质缺损，分实验组和对照组两组进行钢板内固定并植骨，于术后4、8、12周对实验组及对照组行X线正位片检查，观察骨折愈合过程中不同时间点的影像学特点。于4、8、12周各组处死4只动物行大体标本观察，并取缺损区骨组织行组织学检查。X线检查及组织学检查结果根据LaneX线评分标准及组织学评分标准进行评分。最后采用SPSS13.0进行统计学分析。

OBJECTIVE: To propose an improved method to calculate the cross-sectional area of the vertebra, and validate the correctness of the method.

目的：改进生物力学实验中椎体横截面积的计算方法，拟通过动物实验说明此方法的准确性。

Animal experiments are necessary in view of their medical value, but they should be carried out within the scope of tolerableness.

因为具有医学价值，所以动物实验是必要的，但实验应该在可以容忍的范围内进行。

Methods:Anti-inflammation of XSG was observed by mouse model of auricle swelling and rat model of ankle joint swelling caused by xylol and egg-white respectively,and analgesia was tested by hot-plate caused sole-ache and acetic caused bellyache.Gastric ulcer model rats was duplicated in by Reserpine and Acetate.

采用小鼠二甲苯性耳廓肿法、大鼠蛋清性关节肿法进行抗炎实验观察；采用小鼠醋酸扭体法、热板法进行镇痛实验观察；用利血平、腹腔注射及冰乙酸直接胃壁注射复制大鼠胃溃疡模型，观察香砂六君颗粒对两种胃溃疡动物模型的溃疡指数的影响并计算溃疡抑制率。

Results In animal study, lumbricus could inhibit the growth of staphylococci, bacillus coli and bacillus aeruginosus. The time of wound healing in experimental group was 4 days shorter than that in control group. At 4d and 7 day the numbers of the capillary, blood vessel endodermis and desmohemoblast desmocyte and splitting epithelium of trial group were much more than those of control group. At 4d the trial group′s numbers of splitting mesenchymal cell were much more than that of control group. From 3d on the wound healing and granulation filling of experimental group were much quicker than those of control group.

结果 动物实验中，地龙能抑制金黄色葡萄球菌、大肠杆菌及绿脓杆菌的生长；实验组创面愈合时间较对照组提前了4天；第4、7天实验组动物创面的毛细血管数、血管腔内皮细胞数及间质成纤维细胞数均较对照组明显增多，上皮细胞分裂象也高于对照组，第4天的间皮细胞分裂象也高于对照组；自第3天始创面愈合及肉芽充填速度实验组明显快于对照组。

Methods: An animal model of Low pressure of upper airway was proposed by use of the little type pig, and the tension-stretch ratio relationships of the pharyngeal soft tissues such as cavum nasopharyngeum, soft palate and staphylion were measured in vitro through uniaxial tensile experiments.

用小型猪建立上气道低阻力动物模型来模拟阻塞性睡眠呼吸暂停综合征症状，对动物模型的咽组织（包括鼻咽腔软组织、软腭和悬雍垂）进行单轴拉伸实验，离体检测各部分的弹性，并和正常情形进行对比。

In traditional behaviour study the lack of effective measurement and analysis led to time taking, recorder disturbing, misplaying and inferior quantification.

本研究强调医工结合，充分利用现代生物传感、电子、计算机多媒体等高技术，在声光电屏蔽与严格温湿度控制的实验室内，对动物运动行为进行长时间无干扰监测分析，整个记录和初步统计过程完全由计算机自动控制完成，克服了现有行为学研究方法对动物产生的&二次干扰应激反应&等缺点。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。