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动物力学

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Methods:Animal models of 25 adult mixed breed dogs were established by transverse osteotomy at bilateral iliopectineal crest,which were fixed with ATMFS anterior column connector and steel plate respectively. The animals were examined at 2,4,6,8,and 12 weeks by X-ray,macropathological observation and biomechanical test. Tetracin fluorescent labeling,HE staining,Masson triad colour staining,in situ hybridization and immunohistochemical method were performed and histological image analysis were applied.

建立犬双侧骨盆弓状线骨折的动物模型,分别采用ATMFS前柱固定器和5孔重建钢板内固定,于术后2、4、6、8、12周分别行影像学检查、大体观察及生物力学测试;采用四环素荧光标记法、HE染色、Masson三色染色法、原位杂交法、免疫组织化学法进行组织学观察并图像分析;采用RT-PCR法检测骨折端不同时期骨钙蛋白和核心转录因子mRNA相对表达量的变化。

It is primarily dedicated to education and research on biology including Ecology and Evolution, Biomechanics and Comparative Physiology, Animal Sciences, Plant Sciences, Molecular and Cellular Biosciences, Reproductive Biology, Pathobiological Sciences.

主要致力于生物学科的教育与研究,学科领域包括生态学和进化、生物力学和比较生理学、动物科学、植物科学、分子和细胞生物学、生殖生物学、病原菌科学。

Purpose The clinical prospects of injectable calcium sulfate were verified through the mechanical tests, degradation and osteogenesis abilities' experiments, and the physicochemistry characteristics and biomechanical appreciation were evaluated.

对可注射式硫酸钙理化性能和生物相容性进行检测,通过动物实验研究其力学稳定性、降解性能及成骨性能,探讨可注射式硫酸钙的临床应用能力。

Objective To propose the mechanical method and design the experimental devices for establishing the saccular aneurysm animal model.

目的提出一种力学办法,并设计一套实验装置建立囊状动脉瘤动物模型。

Objective To examine biomechanic change of vessel wall and re-stenosis after dilation of stenosed vena cava in dogs and to infer possible mechanisms of hypotonic luminal benign stenosis in an attempt to provide new evidence for clinical study of potential mechanisms of vena cava stenosis.

目的 通过实验动物研究,初步探讨下腔静脉狭窄扩张后的管壁生物力学特征的改变,推测再狭窄的可能机制。

Rabbits were sacrificed at 12 weeks.The maximum loading of the femur-graft-tibia complexes were determined in 858 Mini BionixⅡtestor,and the rupture site was observed.

于术后12周处死动物,在858 Mini BionixⅡ型生物力学测试机测定股骨-移植物-胫骨的复合极限载荷、断裂部位。

Objective: This experimental study is based on earlier animal and clinical experiment, drawn thinkings of combinations with two methods and pursued a synthesis method of high-efficiency, cheap and convenient calcium and exercise treatment. We adopt taking natural-clam's casing powder and preconcereted-intensity excises to intervene shape of osteoporosis in gelded rats, than we compare the indexes such as the biomechanics indexes of the right femur and the third lumbar vertebrae, the weight and uterus quotiety of rats before the experiment to these indexes after the experiment. We hope to find better and suitable way to prevent and treat the postmenopausal osteoporosis and offer reference for clinic.

目的 本实验的主要目的是在前期动物实验的基础上,引入两种方式联合干预的思维,立足于探求既高效又经济、方便的补钙剂加上与运动疗法相联合的方式,对切除雌性大鼠卵巢而复制成的骨质疏松症模型进行服用天然贝类蛤蜊壳粉,同时施以预定强度的运动刺激的方法,再通过试验前后大鼠体重、子宫指数的变化及大鼠股骨和椎体生物力学指标的对比变化进行分析,以期探索出防治绝经后骨质疏松症的更好的、更适宜于大众的手段;并为天然钙剂和运动联合作用防治PMOP提供参考。

The coil spring were kept constant and recorded for 0hr, 1 hr, 2 hrs, 4 hrs, 8 hrs, 12hrs, ldays, 3days or 7days. Upon completion of experiments, the maxillae were removed. The specimens were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer for 24 hrs and decalcified in 10% ethylene diamine tetraacetic acid at room temperature for 5 wks.

本研究通过建立正畸大鼠牙齿移动动物模型和体外细胞培养,结合细胞力学试验方法和分子生物学测试技术,拟从组织、细胞水平以及分子生物学角度探讨ATF4在正畸牙齿移动过程中对牙周组织改建的影响及作用机制,进一步为正畸牙齿移动提供理论和实践依据。

After three months, gained blood from cardinal vein and detected the level of serum E2; put all rats to death and got femur bone tissue with asepsis condition. Detected the transcriptional level of PTHR1 gene with RT-PCR. And detected the expression level of PTHR1 protein with Western blot. Results: In ovariectomized groupthe level of serum E2 was 13.80±1.10 pmol/L, the E2 of model control group was 23.51±1.20 pmol/L.

术后3个月腹主静脉取血,测定各组大鼠血清E2水平;分别用小动物骨骼强度测定仪和双能X线骨密度仪检测右股骨生物学力学和骨密度;从左股骨中提取总RAN和总蛋白,分别检测PTHR1在转录和翻译水平上的变化。

Adult beagles were used as the animal model. To establish the lidocaine release profile, DAHs were first placed in extraction sockets, then removed at various time intervals of 10, 30, 60, 90, 120, 150, or 180 minutes.

本研究之目的是应用米格鲁犬之动物试验模式,活体评估DAH之利度卡因药物释出动力学,及组织学伤口愈合效果。

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