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At the same time,the morphological variations of the ampulla and ithmus of the oviducts were observed and measured by light microscopy. Results The serum levels of estradiol were E>PE>ME>DE>OVX.The differences between the two groups were significant.The height of the oviductal epithelia was E>PE>DE>ME.After ovariotomy,the epithelia of the oviduct were atrophied and regressive.

结果 大鼠血清雌二醇浓度依次为:动情期组>动情前期组>动情后期组>动情间期组>去卵巢组,各组间差异均有显著性;输卵管粘膜上皮高度在动情周期中变化为:动情期组>动情前期组>动情间期组>动情后期组;去卵巢后,输卵管粘膜上皮萎缩。

Using orthogonal experimental design with orthogonal layout of L16(45), effects of cadmium(0,100,1000,2000μmol/L) and estrous cycle(proestrous,estrous, postestrous, dioestrous)on steroidogenesis in the ovary were studied.

采用正交表L16(45)设计正交实验,研究镉(0、100、1000、2000μmol/L)和动情周期(动情前期、动情期、动情后期、动情间期)对卵巢切碎组织分泌E2和P4的影响。3。

Methods Using orthogonal experimental design L16(4^5, two factorial experiments were designed to explore the influence of four dosage levels of vitamin D30, 10^(-4,10^(-7),10^(-10)mol/L and four different stages (proestrous, estmus, postestrous, dioestrous)of estrous cycle on steroidogenesis in the ovary.

采用正交表L16(4^5)设计实验,研究VD3(10^(-4), 10^(-7), 10^(-10)mol/L在动情周期的4个阶段(动情前期、动情期、动情后期、动情间期)对卵巢切碎组织分泌E2和P4的影响。

The analysis of regression showed that there was a linear regression between the estradiol level in serum and the height of the oviductal epithelia.

上述两项检测结果经回归分析发现,从动情间期至动情前期再至动情期两者呈正相关。

In this experiment, the amount of NE in Hb of preestrus is obviously higher than that in estrus and diestrus.

本实验结果表明,动情前期Hb内NE含量明显高于动情期和动情间期

The level of UEA receptor at diestrus was much higher than that at estrus and that of pregnancy.

动情间期UEA受体水平明显高于动情期和孕期(P<0.01)。

The level of the ECL and the PHA-L receptors as higher at the estrus than that at the diestrus. ECL and PHA-L receptors maintained the lower level at the day 4 and 5 of pregnancy than those at the diestrus.

ECL和PHA-L受体水平在动情期高于动情间期,孕期呈现先下降后上升的趋势,且在孕4、孕5d的水平显著低于动情间期和动情期(P<0.01)。

During peri-implantation, the decrease of the ECL and the PHA-L receptors may lead to the failure of implantation in the oviduct, and the increase of the UEA receptors at diestrus may contribute to the process of fertilization.

围植入期ECL和PHA-L受体表达下降,在输卵管内形成了不利于植入的微环境,可能是胚泡正常情况下不植入输卵管的原因之一;动情期UEA受体水平显著低于动情间期,有利于受精过程的正常进行。

During periimplantation, the decrease of the ECL and the PHAL receptors may lead to the failure of implantation in the oviduct, and the increase of the UEA receptors at diestrus may contribute to the process of fertilization.

围植入期ECL和PHAL受体表达下降,在输卵管内形成了不利于植入的微环境,可能是胚泡正常情况下不植入输卵管的原因之一;动情期UEA受体水平显著低于动情间期,有利于受精过程的正常进行。

Methods According to the examination result of vagina smears, 12 female rats were killed in proestrus, estrus, metaestrus and diestrus respectively, 3 for each, and their uterus and ovary were taken out, from which the total RNA and total protein were extracted for determination of transcription of SLC26A3 mRNA by RT-PCR, and expression of SLC26A3 protein by Western blot.

根据阴道涂片检测结果,将12只雌性Wister大鼠分别在动情前期、动情期、动情后期和动情间期断头处死,取出卵巢及子宫,提取总RNA及总蛋白,用RT-PCR及Western blot方法检测卵巢及子宫内SLC26A3在动情周期中各时期的表达。

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