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The amount of LHRH in pituitary and hypothalamus in preestrus is also higher than that of the other.

而动情前期垂体和下丘脑LHRH含量也明显高于其它两个期,处于较高水平。

Then replate them onto feeder layer culture system (containing leukemia inhibitory factor) in order to passage and expand. Subsequently we identified the expanding cells by alkaline phosphatase staining, karyotype analysis and the formation of embryoid body which are typical characteristics of ESCs.

以分离培养的细胞作为供体细胞,近交系C57BL/6J为受体囊胚供者,采用囊胚注射法构建嵌合胚,体外恢复培养后移植进动情期子宫中,结果生出了黑白杂合的嵌合鼠。

The method is taken not pregnant estrum and pregnant 1 D, 2 D, 3 D, 4 D in the morning 4, D afternoon 4, the small rat uterus of D midnight, 5 D, 6 D, 7 D, 8 D makes section, with immune organization chemical coloring and image analyse a technology to be to STAT3 inside uterus of early pregnant small rat velar expression undertakes detecting.

方法取未孕动情期及孕1 d、2 d、3 d、4 d上午4、d下午4、d午夜、5 d、6 d、7 d、8 d的小鼠子宫做切片,用免疫组织化学染色和图像分析技术对STAT3在早孕小鼠子宫内膜的表达进行检测。

Methods: 4~6 months old SD female rats were set to be normal control group and 10~12 months old SD female rats whose oestrus elongation were set as primary senile female rat model.

4~6个月龄雌性大鼠为正常对照组;10~12个月龄,阴道细胞学表现动情期延长的雌性大鼠作为初老大鼠模型。

Results In the follicles of estrous cycle rat,the signals of MMP 2mRNA were detected in the granulosa cells and thecal cells;w...

结果 在动情周期中,各情期卵巢中卵泡的颗粒细胞和发育良好的卵泡的膜细胞中均可检测到MMP 2mRNA的杂交信号;MMP 9mRNA的表达始于已发育良好的窦前卵泡的膜细胞,此后在小窦状卵泡、窦状卵泡及排卵前卵泡的膜细胞中均检测到MMP 9mRNA的杂交信号。

Methods:Endometria of mice in the early phase of pregnancy and estrus cycle were collected and divided into 8 study groups,and every group included 10 mice.

收集动情周期各期和早孕2 d、3 d、4 d、5 d小鼠子宫内膜,共8组,每组10只小鼠。

It was shown that: 1 MMP2 activity reached the highest level during proestrus, and was lowest during diestrus; 2 MM2 acti vity was increased from the first day of pregnancy, peaked on day 4 of pregnancy; but decreased significantly at day 7 of the pregnancy. 3 MMP2 activity was obviously higher on day 1 of pseudopregnancy than on the other days, and its activity decreased gradually, reached the very low level at day 4 of pseudopregnancy and maintained the low level until day 7; 4 MMP2 activity was decreased significantly 24 hours after injection of 17βestradiol ( 01 mg) at metaestrus, while MMP2 activity had no change after progesterone (04 mg) injection.

明胶酶A活性变化如下:1在大鼠的动情前期明胶酶A的活性最高,间情期最低;2在妊娠早期(1~7天)的卵巢中,明胶酶A的活性在妊娠第4天达到最高,第1~3天及第5、6天其活性也较高,但第7天则明显下降;3在假孕(1~7天)大鼠的卵巢中,明胶酶A的活性在假孕第1天最高,第2、3天略下降,从第4天到第7天,其活性都处于较低水平;4动情后期大鼠皮下注射雌二醇(0.1mg/只)24小时后,明胶酶A活性明显上升。

Forty SD female rats were divided randomly into four groups : control (received normal saline, CTX, S1P+NS, S1P+CTX respectively. All rats were killed between the first and the second week in the diestrus stage after stopping medicines to compare the number of the primordial follicles,the primary follicles and the growth follicles. The expression of the Bcl-2 and Bax mRNA in the ovaries were examined by the RT-PCR.

40只雌性SD大鼠随机分为4组,即实验对照组、CTX组、 S1P+ NS组及S1P+CTX组,每组10只,于结束用药的第1~2周内的动情间期处死,观察卵泡数量的变化,并采用半定量RT-PCR检测卵巢组织Bcl-2及Bax 的mRNA变化。

Using immunohistochemistry SABC technique, the location, distribution and density of nerve fibers immunoreactive to nitric oxide synthase Ⅰ were studied in the genital organs of the female Wistar rats during diestrus.

摘 要:实验采用亲和免疫组化SABC法对雌性大鼠动情间期一氧化氮合酶Ⅰ的正常分布进行了观察。

Results The expression of endogenous LN was found in the basement membrane of epithelium, glands and blood vessels in uterine endometrium of both estrus and early pregnancy. And its was faint that LN present in basement membrane as the implantation occurs, but the LN immunostaining in the epithelial cells of endometrium was stronger. The exogenous LN has no obvious influence on blastocyst attachment and implantation. LN-antibody intra-uterine injection showed significant repressing effect on mouse blastocyst implantation.

结果 动情期及妊娠早期小鼠子宫内膜内源性LN在上皮和腺基膜及血管内皮基膜均有表达,并随着植入的发生基膜的LN表达减弱,而内膜上皮中LN免疫染色逐渐增强;外源性LN对小鼠胚泡粘附与植入子宫内膜不产生明显影响,层粘连蛋白抗体明显抑制小鼠胚泡着床。

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