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动情周期

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METHODS:The 12~14 months old rats which sexual circle being disorder were chosed;the groups were Yanfuchun 5.0 g.kg-1,Yanfuchun 10 g.kg-1,Gengnianan 1.1 g.kg-1,model group and young controls;the rats were administered in continuousness orally Yanfuchun 5.0,10.0 g.kg-1,Gengnianan 1.1 g.kg-1 and the same volume of water for 22 days respectively.

选用12~14月龄动情周期紊乱的雌性大鼠进行实验,实验分延妇春5.0、10 g.kg-1;更年安1.1 g.kg-1,模型组及青年对照组;分别给大鼠连续灌胃延妇春5.0 g.kg-1。延妇春10 g.kg-1;更年安1.1 g.kg-1及等量蒸馏水22天。

Endometriosis was surgically induced in 80 SD rats, The growth of the transplant and rats' estrus cycle was observed in different times after the implantation operation. The endometrum of the uninjured side and the ectopic endometrum were taken for observing by microscope and electroscope.

采用外科诱导法对80只大鼠行建模手术,在术后的第3周、第5周、第7周和第12周动态观察病灶的生长情况和大鼠的动情周期变化,取健侧子宫角中段的在位内膜、异位内膜行组织学研究。

At the same time,the morphological variations of the ampulla and ithmus of the oviducts were observed and measured by light microscopy. Results The serum levels of estradiol were E>PE>ME>DE>OVX.The differences between the two groups were significant.The height of the oviductal epithelia was E>PE>DE>ME.After ovariotomy,the epithelia of the oviduct were atrophied and regressive.

结果 大鼠血清雌二醇浓度依次为:动情期组>动情前期组>动情后期组>动情间期组>去卵巢组,各组间差异均有显著性;输卵管粘膜上皮高度在动情周期中变化为:动情期组>动情前期组>动情间期组>动情后期组;去卵巢后,输卵管粘膜上皮萎缩。

Using orthogonal experimental design with orthogonal layout of L16(45), effects of cadmium(0,100,1000,2000μmol/L) and estrous cycle(proestrous,estrous, postestrous, dioestrous)on steroidogenesis in the ovary were studied.

采用正交表L16(45)设计正交实验,研究镉(0、100、1000、2000μmol/L)和动情周期(动情前期、动情期、动情后期、动情间期)对卵巢切碎组织分泌E2和P4的影响。3。

Methods Using orthogonal experimental design L16(4^5, two factorial experiments were designed to explore the influence of four dosage levels of vitamin D30, 10^(-4,10^(-7),10^(-10)mol/L and four different stages (proestrous, estmus, postestrous, dioestrous)of estrous cycle on steroidogenesis in the ovary.

采用正交表L16(4^5)设计实验,研究VD3(10^(-4), 10^(-7), 10^(-10)mol/L在动情周期的4个阶段(动情前期、动情期、动情后期、动情间期)对卵巢切碎组织分泌E2和P4的影响。

The experimental results proved that TT gradually made the irregular estrous cycle of MP mice regular.

实验结果表明,TT能使MP小鼠无规则的动情周期逐渐恢复正常,表明TT对MP小鼠动情周期的紊乱有改善作用。

RESULTS: 52.17% of the rats in fresh embryo transfer group and 38.01% in frozen embryo transfer group resulted in restoration of regular estrous cyclic activity. Serum oestradiol and progesterone levels became normal on day 35 and day 49 after transplantation.

结果:新鲜移植组和冷冻移植组分别有52.17%和38.01%大鼠恢复了动情周期,两组血清雌二醇和孕酮在分别在35和49 d恢复到正常,且动情周期天数与正常对照组比较,差异无显著性意义。

Estrus,also oestrus:The periodic state of sexual excitement in the female of most mammals

&动情,动情周期:大多数哺乳动物的雌性产生的周期性的性冲动,在此期间内雌性具有排卵的优势,所以该期间最适合性交;刺激&

Methods According to the examination result of vagina smears, 12 female rats were killed in proestrus, estrus, metaestrus and diestrus respectively, 3 for each, and their uterus and ovary were taken out, from which the total RNA and total protein were extracted for determination of transcription of SLC26A3 mRNA by RT-PCR, and expression of SLC26A3 protein by Western blot.

根据阴道涂片检测结果,将12只雌性Wister大鼠分别在动情前期、动情期、动情后期和动情间期断头处死,取出卵巢及子宫,提取总RNA及总蛋白,用RT-PCR及Western blot方法检测卵巢及子宫内SLC26A3在动情周期中各时期的表达。

According to the WHOQOI-100 and WHOQOL-BREF measuring scale about quality of life , the state of quality of life were observed in these two groups; The visual vague scale was adopted to survey the degree to improve the dysmenorrhea.3 Animal Experiment 60 healthy sanitation female Wistar rat with normal estrous cycle were selected,200±20g in weight,according to the result of vaginal smear examination, make the model with operation at estrum to duplicate the model of endometriosis according to the Wang Yan-ping's method, the abdomen of the sham operated group were opened and closed with no management.

参考WHOQOI-100和WHOQOL-BREF生存质量测定量表,观察两组患者的生活质量;采用视觉模糊评分法观察患者的痛经改善程度。3动物实验选用动情周期正常的健康清洁级雌性成年Wistar大鼠60只,体重200±20g克,经阴道涂片检查,在大鼠的动情期进行手术造模。

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