加酶的

During mixed fermentation, two peaks existed in production of 2-keto-L-gulonic acid, including 8～20 hours and 24～40 hours; the activity of L-sorbose dehydrase increased rapidly and 2-keto-L-gulonic acid reductase slowly.

在流加糖发酵过程中，2-酮基-L-古龙酸的合成有两个高峰期：8～20小时和24～40小时高峰期。L-山梨糖脱氢酶总酶活迅速上升，2-酮基-L-古龙酸还原酶活性始终呈平缓上升趋势。

Objective To investigate the role of heme oxygenase/carbon monoxide pathway in the development of renal hypertension.

目的 探讨肾性高血压时血红素加氧酶/一氧化碳系统的变化，以明确血红素加氧酶在肾性高血压发病过程中的意义及作用。

Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果，选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验，采用快速银染法进行染色，随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索，鉴定了3个蛋白，它们是：1-氨基环丙烷-1羧酸合酶2，核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

Results When cellulase and pectinase compound,amlyse and diastatic enzyme were added in order in the method of stepwise biocatalysis,98% diosgenin from plants was extracted.IR spectrum of the product was in accordance with that of reference substance and the purity of it is more than 95% according to its HPLC chromatogram.

结果利用阶梯生物催化法，当纤维素酶和果胶酶复合酶制剂、淀粉酶以及糖化酶按顺序依次加入时，能够将植物中98%的薯蓣皂苷元提取出来，其产品的红外谱图与对照品谱图相吻合，经HPLC谱测定薯蓣皂苷元质量分数95%以上。

Alexander has demonstrated an iodide peroxidase in animal thyroid tissue and believes this enzyme is responsible for the iodination reactions through formation of an oxidized iodinating intermediate.

Alexander已经证明在动物甲状腺组织中有一种加碘过氧化物酶，并相信该酶对加碘反应起作用，它是通过形成一个氧化态的碘中间体而起作用。

The results showed that the activities of catalase, ascorbate peroxidase, peroxidase and dehydroascorbate in apoplast of cucumber leaf inoculated with Sphaerotheca fuliginea were significantly increased, but the activities of superoxide dismutase was obviously decreased; Silicon can remarkably increase the activities of APX, SOD, POD and DHAR and decrease the activity of CAT.

结果表明，诱导接种后，质外体中过氧化氢酶、抗坏血酸过氧化物酶、过氧化物酶及脱氢抗坏血酸还原酶活性显著升高，超氧化物歧化酶活性明显降低；加硅显著提高了APX， SOD， POD和DHAR的活性，降低了CAT活性。

Versicolor suited to grow and produce laccases at pH value 3.5-5.7, and unbuffered media were able to get good laccase productivity though buffered media could get higher laccase activity. Metal ions Cu(superscript 2+) and Mn(superscript 2+) in the culture could remarkably increase the activity of laccases. The optimal concentration of Cu(superscript 2+) was 0.008-0.08mmol/L, and the optimal concentration of Mn(superscript 2+) was about 0.01mmol/L, when the culture contained 0.1mmol/L 2,5-dimethoxyaniline.

结果发现该菌种适宜在pH值3.5~5.7环境下生长并合成漆酶，培养基中添加缓冲液可以获得更高的酶活，但不加则有较高的酶产率；铜和锰可以显著提高漆酶活力，当以0.1mmol/L 2，5-二甲基苯胺为诱导剂时，培养基中宜含0.008~0.08mmol/L Cu(上标 2+)，0.01mmol/L左右的Mn(上标 2+)。

He analysis of the category and function of TCB stress inducible proteins showed that different groups of proteins were induced by 5 mg L^(-1) TCB stress. They are detoxification enzymes (including esterase, aldo/keto reductases, and glutathione S-transferase), cell wall compound metabolism related enzymes (including UDP-glucose protein transglucosylase and GDP-mannose 3,5-epimerase 1), phytohormone metabolism and regulation related enzymes or proteins (including aci-reductone dioxygenase 4, beta-glucosidase, two members of pathogenesis-related proteins from family 10), primary and secondary metabolism regulative enzymes (including translational elongation factor Tu, cytosolic orthophosphate dikinase, triosephosphate isomerases, alanine aminotransferase, and isoflavone reductase).

CB胁迫能诱导根系内不同类型蛋白质的表达，它们是：解毒酶（包括脂酶、醛／酮还原酶、谷胱甘肽－硫转移酶），细胞壁物质代谢相关酶（包括蛋白转葡萄糖基酶、GDP-甘露糖－3，5-异构酶1），激素代谢或调节相关酶（包括酸式－还原酮二加氧酶、β-葡萄糖苷酶、病程相关蛋白家族10中的2个不同蛋白），原初或次生代谢相关酶（包括转录延长因子、细胞质磷酸丙酮酸双激酶、磷酸丙糖异构酶、丙氨酸氨基转移酶和异黄酮还原酶）。

Vmutageniz, We gained a stain of Aspergillus niger S13 with high cellulase production, Further more we research on the optimum solid culture a wheat stem 70 % bran 30 %, nitrogen source is yeast extract 0.68%,(NH4)2SO4 0.35%, trace element 0.1%, Vogel's solution 4 %, pH3.5, temperature 30℃, cultivation time 72-96hr, With such condition, its β-glucosidas activity reached 801.18mg·glucose/g·h, CMCase activity 6262.46mg·glucose/g·h filter paper activity 20.73IU.

本文通过分离筛选和紫外诱变法，选充出一株高产纤维素酶黑曲霉菌株S13，并进行了最佳固态发酵培养条件的研究，其结果为：最佳碳源为1％碱洗麦杆粉70％，麸皮30％；另加酵母膏0.68％，(NH_4)_2SO_4 0 35％，微量元素0.1％，Vogel's母液4％，初始pH3.5培养温度30℃，培养时间为72～96 小时，在此条件下其β-葡萄糖苷酶活力达801.18mgG/h·g，CMC酶活6262.32mgG/h·g，滤纸酶活20.73IU。

To obtain high cell density and high yield of MTG by Streptomyces hygroscopicus, the effects of exponential feeding of glucose and the addition of nitrogen source on MTG production were investigated.

在吸水链霉菌分批发酵研究的基础上，通过在菌体生长阶段指数流加葡萄糖，进行高细胞密度培养，获得了较高的菌体量；待菌体生长进入产酶期后，通过补加氮源，为产酶提供充足的氮源，其中通过流加蛋白质氮源，可以减少蛋白酶对成熟MTG的分解，促进产酶。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。