加酶的

Then select the very surfactants\' concentration to treat SBP for one hour, scan on the circular dichromatism spectrum and fluorescent spectrum; ultraviolet-visible spectrum scans SBP\'s variations within 2h after surfactants\' adding to immediately.

实验前加适量相应缓冲液溶解使SBP浓度为250mg/ml.30℃下，设置三种表面活性剂的浓度梯度，使得每种表活剂的加入使得SBP酶活力变化，并在某一特定浓度和特定时间残留酶活力稳定。

RESULTS: The occurrence rate of skin damage was significantly lower in hyaluronidase group and hyaluronidase plus chitosan group than in chitosan group, saline embrocation group, saline injection group, and control group 30% and 20% vs.

结果：透明质酸酶组和透明质酸酶联合几丁糖组的损伤发生率分别为30%和加20%，显著低于几丁糖组、生理盐水外涂组、生理盐水注射组和模型对照组的损伤发生率(90%、100%、90%、100%)(P.05)。

The zingibain obtained by UF can have beer and red wine clearfied. The optimal concentration of zingibain for both beer and red wine was 0. 05mg/L and the pre treatment temperature was 60 癈 in beer and 20~~30C in red wine. The ginger juice did not clarify beer and red wine.

用超滤法提取并冻干的生姜蛋白酶粗制酶对红葡萄酒、啤酒进行了澄清试验，结果表明：生姜蛋白酶能明显提高红葡萄酒、啤酒的澄清度，其最适加量，红葡萄酒、啤酒都为0.05mg／L，最佳作用温度啤酒为60℃，红葡萄酒为20～30℃；姜汁不宜作为酶源直接用于红葡萄酒和啤酒中。

Methods: Liver microsome was preparated from normal rat and human liver, which was to he treated with a series of concentrations of CsA or coadministration with Flu or MP. The activities of CYP3A in the microsomes were detected by using erythromycin as substrate.

製备大鼠和人肝微粒体，用不同浓度的CsA作用、加或不加MP、Flu，以红霉素为底物，经过NADPH系统孵育后，利用分光光度法测定人和大鼠肝微粒体的红霉素N-脱甲基酶活性。

Some preparation methods of galanthanime including extraction and organic synthesis were summarized, such as extraction from narcissus, galanthus woronawii losink, and lycoris radiate and countereurrent chromatography refining, and enzymatic extraction from hyacinths orientalis, as well as the organic synthesis using 3,4-dimethoxybenzaldehyde, methyl 3,4,5-trihydroxybenzoate, isovanilin and tyramine as raw material.

概述了加兰他敏从黄水仙、沃氏雪花莲中提取、石蒜中提取及逆流色谱法精制、喇叭水仙酶法中提取的方法、工艺；以3，4-二甲氧基苯甲醛、3，4，5-三羟基苯甲酸甲酯和异香草醛和酪胺为起始原料合成加兰他敏的方法。

In which the buffer's pH was adjusted, pfu Taq DNA polymerase and Klenow fragment were combined, the organic reagent methylamine was added to uncoil the secondary structure.

本研究通过调整反应体系缓冲液pH值、采用高保真pfu DNA聚合酶和Klenow酶组合、加缓解二级结构的有机试剂甲胺、换引物多步扩增而成功实现了重复区片段R02一R11的扩增。

It was difficult to extract corn pigments completely, for some of them were in a protein-bind state. It was better to use active carbon for the decolorization of GEON, the conditions were 40℃, stir time 6 hours, the ratio of carbon to hydrolyzate 1: 100 , but as to milk-like powder, it was better to extract pigments after spray drying.

最后，本文对玉米黄素的可抽提性也进行了初步研究，结果表明，部分色素与蛋白质结合而难以抽提，因而有机溶剂难以将色素抽提完全，对Gln活性肽营养液而言，可选用酶解后加活性炭进行脱色，脱色条件为40℃，搅拌6小时，炭液比为1∶100；而对黄粉蛋白乳粉而言，喷雾干燥后用乙酸乙酯脱色，可使酶解干燥后黄粉的异味减轻。

METHODS: The spiral modiolar artery in guinea pig cochlea was exposed by microsurgery, then the fluorogold or horseradish peroxidase was given around the SMA for retrograde tracing.

在正常豚鼠单侧耳蜗螺旋蜗轴动脉局部滴加逆行追踪剂辣根过氧化物酶或荧光金，观察双侧交感颈上神经节、星状神经节内HRP或FG逆标神经元的分布；采用逆标与免疫组化相结合的双重标记方法观察追踪阳性神经元是否表达酪氨酸羟化酶、神经肽Y、钙基因相关肽或P物质。

Methods: the spiral modiolar artery in guinea pig cochlea was exposed by microsurgery, then the fluorogold or horseradish peroxidase was given around the sma for retrograde tracing. the bilateral superior cervical ganglia and stellate ganglia were observed to find retrogradely labeled neurons. the neurotransmitters of these sympathetic fibers around sma were ascertained by using retrograde tracing combined with immunohistochemical double labeled technique.

在正常豚鼠单侧耳蜗螺旋蜗轴动脉局部滴加逆行追踪剂辣根过氧化物酶或荧光金，观察双侧交感颈上神经节、星状神经节内hrp或fg逆标神经元的分布；采用逆标与免疫组化相结合的双重标记方法观察追踪阳性神经元是否表达酪氨酸羟化酶、神经肽y、钙基因相关肽或p物质。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle， GV)卵母细胞；用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达：其中cDNA的合成分两种方法进行：一是将GV期单个卵母细胞直接进行RT合成cDNA，二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT；回收产物构建克隆质粒并测序。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。