加酶的

Ribusco-1,5-bisphosphate carboxylase/oxygenase located in the chloroplast is the most abundant protein in the leaves of light-grown plants.

1，5-二磷酸核酮糖羧化酶／加氧酶位于植物叶绿体中，是绿色植物中含量最丰富的蛋白，它催化CO2固定和光呼吸作用的第一步。

Hence, the exponential feeding mode of nutrients was carried out to control the catabolite repression by limiting the specific cell growth rate.

指数流加结果显示，通过限制细胞的比生长速率，可将阻遏作用控制在较低水平，酶产量得到了大幅度提高，是分批发酵酶产量的7.9倍，达到2565U/mL。

DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA，目的DNA的聚合酶链反应扩增及电泳检测，5-羟色胺2A受体基因T102C基因片断扩增的引物序列：5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3'；采用50 μL反应体系，DNA 0.5 μg，引物50 pmol，TagDNA酶2U加dNTP至终浓度200μmol/L。

Methods: 1. To detect the GC level in the serum by enzyme immunoassay kit.The serum samples were obtained from 22 psoriatic patients, 15 patients who had been clinical cured and 20 normal controls. There were standard, control, and sample wells. The standardN control and serum samples were pipeted into the appropriate wells and then the enzyme conjugate solution, GC antiserum were pipeted into each wells, incubated the wells at room temperature for 45 minutes, washed and dried each well, added TMB chromogen solution, incubated the wells for 15 minutes, added H2SO4 to stop the reaction.

1。酶免疫法测定银屑病患者治疗前、后血清GC水平的变化：血清标本来自22例治疗前银屑病患者、15例临床治愈的银屑病患者第四军匝大学硕＿］，学位论文和 20名正常人对照，设置标准品孔、对照品孔和样本孔，标准品、对照品和血清样品分别加入对应各孔中，在各孔中再加入酶联GC，兔抗GC血清，室温下放置 45分钟后清洗吸干，加 TMP色原，15分钟后 11p。

The facts that type I compounds could easily undergo deamination reaction to form α-methylene cyclopentanone derivatives and they could react with glutathione to form an adduct provided sufficient evidence for the hypothesis that the compounds acted as masked α-methylenecyclopen- tanones and reacted with biologically important sulfhydry-containing enzymes, and thus produced anticancer activity.

Ⅰ类化合物在稀溶液中极易进行去胺基化反应形成α-亚甲基环戊酮类化合物。并且Ⅰ类化合物于稀溶液中易与谷胱苷肽进行结合，这些事实为Ⅰ类化合物作为隐蔽的α-亚甲基环戊酮与酶进行加成，从而导致酶失活而具有抗癌活性的设想提供了证据。

Methods From the January,2003 to the December,2008,when we were working in the Xuanhan people,s Hospital and Chengdu Geriatric Hospital,The 39 patients diagnosed Binswanger Disease were admitted, Randomized went in treatment group or control group.All in the general treatment,meanwhile,the control group add Shuxuening 15ml,intervenous drop infusion,once a day.The treatment group add Fibrinolysin,iv gtt,once a day.Both using for 7 days.

2003年1月～2008年12月我们在宣汉县人民医院和成都市老年病医院工作期间，共收治皮质下动脉硬化性脑病患者39例，随机分为治疗组和对照组，在"基础治疗"的同时，对照组加舒血宁注射液15ml，静滴，每日1次；治疗组加纤溶酶注射液200U，静滴，每日1次，均用7d。

So,obviously,the Ca and P level of group Ⅰ diet couldnt meet the nutritinonal requirement of fattening egg-type cockerels.

试Ⅱ组和CK组比较，采食量和料重比分别降低3.78%和4.03%，差异均显著(P＜0.05)，日增重和经济效益分别提高0.24%和0.46%，试Ⅱ组的经济效益和CK组接近，但日粮中加入植酸酶后，减少了粪便磷排泄量，有利于控制环境污染，因此育肥蛋公雏宜采用低钙磷加植酸酶日粮。

In higher plants,glycin betaine is synthesis from choline by choline monooxygenaseand betaine aldehyde dehydrogenase.

在高等植物中，甜菜碱是由胆碱经胆碱单加氧酶(choline monooxygenase，CMO)和甜菜碱醛脱氢酶(betainealdehyde dehydrogenase，BADH)催化生成的。

ABA increased activity of RuBPoxgenase and Glycollic acid oxidasee of C〓 Cycle, on the contrary Zeatin decreased it. Beacuse of increasing rate of C〓 cycle, it added to content of Ser and Gly.

ABA可提高RuBP加氧酶及乙醇酸氧化酶活性，加速C〓循环，Zeatin则相反。C〓循环的加强可以使得中间产物丝氨酸和甘氨酸增加。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。