加氨

In the first step of thedouble annulation route, 2-amino-3-carbamoyl-5,6-dihydro-4-pyridones 2 werecreated in high to excellent yields by a formal [5C + 1N] annulation reaction ofketene--acetals 1 with ammonia.

第一步，二硫缩烯酮1和氨源通过[5C + 1N]加成反应产率极高的得到了2-氨基-3-氨基甲酰基-5，6-二氢-4-吡啶酮2。

Based on the addition of vinyl magnesium bromide to the chiral non-racemic synthon 2 derived from-1, an enantioselective total synthesis of-3, the antipode of marine alkaloid halitulin 10 steps, overall yield: 5.3% from (R-1 was achieved using aza-Claisen rearrangement as a key step.

取得的主要结果如下：一、从-苯基甘氨醇1出发，通过已知的哌啶手性合成砌块2的加成反应，建立了经aza-Claisen重排反应进行海洋生物碱halitulin手性分子片断的对映体--isohaliclorensin 3不对称合成的路线。

Based on the addition of vinyl magnesium bromide to the chiral non-racemic synthon 2 derived from-1, an enantioselective total synthesis of-3, the antipode of marine alkaloid halitulin 10 steps, overall yield: 5.3% from (R-1 was achieved using aza-Claisen...

取得的主要结果如下：一、从-苯基甘氨醇1出发，通过已知的哌啶手性合成砌块2的加成反应，建立了经aza-Claisen重排反应进行海洋生物碱halitulin手性分子片断的对映体--isohaliclorensin 3不对称合成的路线。

Synthesis of novel sulfonamide-prolinols and their application on the asymmetric addition of diethylzinc to benzaldehyde.

三、研究了脯氨酸衍生的新型手性磺酰胺-氨基醇的合成及其催化二乙基锌对苯甲醛的不对称加成反应 1。

The effect of several factors on calcium carbonate inhibitionbehavior of polyaspartic acid is discussed in this paper.

结果表明：在低投加量的情况下，分子量为4000的聚天冬氨酸具有最为优良的阻垢性能。

The carbamate solution from the high-pressure scrubber and fresh ammonia are added.

新鲜氨和从高压洗涤塔来的氨基甲酸盐溶液加到。

Urethane adduct was synthesized from decarboxylated cashew nut shell liquid,hexamethylenediisocynate and polyethylglycol,which was then reacted with melamineformaldehyde resin to form aqueous coating material.

采用天然植物油、己二异氰酸酯和聚乙二醇在较温和的条件下反应，制得了水溶性的氨基甲酸酯加成物，以此和低醚化度甲醚化三聚氰氨甲醚甲醛树脂制备了水性涂料。

A preparation method of the visible-light photocatalyst Bi2WO6 nano-powder is characterized by comprising the steps as follows:(1) the selection of raw materials;(2) bismuth nitrate Bi(NO33 question mark 5H2O is added into a citric acid solution to obtain the citric acid solution containing white sediment;(3) EDTA is added into ammonia water which is then added dropwise with the citric acid solution containing the Bi(NO3)3 question mark 5H2O so to obtain a solution of bismuth;(4) the solution of bismuth is added with (NH4)6W7O24 question mark 6H2O solution to form a precursor solution which is then stirred, dried, coked, cooled and ground to obtain decarburized powder;(5) the decarburized powder is roasted for 2h to 4h under 450 DEG C to 550 DEG C to obtain the visible-light photocatalyst Bi2WO6 nano-powder.

可见光催化剂Bi 2 WO 6 纳米粉体的制备方法，其特征在于它包括如下步骤：1原料的选取；2将Bi(NO 3 ) 3 5H 2 O加到柠檬酸溶液中，得到含有白色沉淀的柠檬酸溶液；3将乙二胺四乙酸加到氨水中，并缓慢滴加入含有Bi(NO 3 ) 3 5H 2 O的柠檬酸溶液中，得铋的溶液；4将(NH 4 ) 6 W 7 O 24 6H 2 O溶液加入上述铋的溶液中，组成前驱液；搅拌，干燥，焦化，冷却后，研磨，得去碳的粉体；5将去碳的粉体在450～550℃下焙烧2～4h后，得到可见光催化剂Bi 2 WO 6 纳米粉体。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

Methods Primary brown adipocytes from rat fetuses, and from wild-type neonate mice and neonate mice deficient in the gene encoding protein tyrosine phosphatase-1B (Ptpn1, also known as Ptp1b) were cultured in the absence or presence of TNFα and different nuclear receptor agonists.

原代棕色脂肪细胞自大鼠胚胎、野生型新生小鼠和蛋白酪氨酸磷酸酶-1B基因基因(Ptpn1，也被称作 Ptp1b)缺陷新生小鼠，在加或者不加TNFα和不同浓度核受体激动剂中培养。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了