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Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of M-CSF (25 μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞,在含有25μg/LM-CSF和0,1,10,100μg/LTNF-α的α-MEM培养液中培养5d后,观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成;细胞在含有25μg/LM-CSF和30μg/LsRANKL的α-MEM培养液中进行培养,比较加入和不加入10μg/LTNF-α培养4、5、6和9d后,所形成的TRAP多核细胞的数目和骨吸收面积。

LOSA-1 is an assistant for increasing production of Light olefine with FCC.The result of its comnercial application in No.2 heavy oil FCC unit in Luoyang company shows that it can raise propylene content in liquefied gas.When its equilibrium addition is about 200kg/d with the proportion to fresh catalyst volume of 5%,the propylne content in liquefied gas can keep at 42%~45%. FCC;propylene;assistant LOSA-1;commercial application

LOSA-1助剂是岳阳三生化工公司和国内科研院所开发的增产轻烯烃FCC助催化剂,该剂在中国石化洛阳分公司2号重油催化裂化装置工业应用的结果表明,LOSA-1助剂具有较强的提高液化气中丙烯浓度的性能,当其平衡加入量控制在200kg/d左右、对新鲜催化剂加入比例为5%左右时,液化气中的丙烯体积浓度能够保持在42%~45%。

Through comparing and analyzing the metallurgical effects, proper addition quantity of the modifier was 80 kg per heat. The experiment results showed that the modifier smelted the slag fast and reduced the oxidizability of the top-slag rapidly. Comparing with the original process, the average content of aluminum in the steel was increased by 0.06%, desulphurization ratio was increased by 89.25% and TFe was reduced by 0.6%, then stabilizing the production of aluminum containing steel.

试验中用改质剂代替原工艺中的精炼剂(Q235C钢种),通过对改质剂不同加入量的冶金效果进行对比分析,确定的改质剂加入量为每炉80 kg,试验表明,改质剂化渣快,能迅速降低顶渣的氧化性,与原工艺相比,钢中平均铝含量提高0.06%,脱硫率提高89.25%,渣中全铁降低0.6%,稳定了含铝钢的生产。

The phosphorus oxychloride or phosphorus tribromide N, N-dimethylformamide mix and react to form the Vilsmeier reagent used in the reaction. The newly prepared Vilsmeier reagent is added into the reaction bottle provided with the return condenser and the blender; a reaction raw material N, N-dimethylformamide solution expressed in the reaction formula is added into the system; the temperature is risen; the corresponding multi substituting pyridine-2 (1 H)-ketone compound can be made after the column chromatography of the silica gel; the production rate is between 60 and 95 percent according to different reactions.

由三氯氧磷或三溴化磷N,N-二甲基甲酰胺混合获得反应中所用Vilsmeier试剂;将新配制的Vilsmeier试剂加入装有回流冷凝管、搅拌器的反应瓶中,向体系中加入反应通式中表示的一种反应原料的N,N-二甲基甲酰胺溶液,升温,经硅胶柱层析分离得相应的多取代吡啶-2(1H)-酮类化合物,产率视不同反应在60~95%之间。

1MM GTP was mixed with tubulin (2mg/ml) and varying concentrations of sodium selenite. The mixture was incubated at 37℃ for 1hr. MTs were separated from unassembled tubulin by centrifugation. Both supernatants and pellets were subjected to SDS-PAGE.

其中一组实验是在相同的微管蛋白体系中加入不同浓度的亚硒酸钠,然后加入GTP,37℃聚合,离心,沉淀为聚合的微管,而上清液为未聚合的微管蛋白。

Adding sugar to the three raw starch and their low cross-linkedsuccinylated starch,the viscosity and thermal paste viscosity were rised eventually; addingcitric acid, lactic acid and sodium chlorid to the three raw starch and their low cross-linkedsuccinylated starch, the final viscosity and thermal paste viscosity showed varying degreesof decline.

加入蔗糖使的三种原料淀粉及其低交联酯化淀粉的热糊粘度与最终粘度有所上升;加入柠檬酸、乳酸、氯化钠后使得三种原料淀粉及其低交联酯化淀粉的热糊粘度和最终粘度呈现不同程度的下降。

The digital watermark was hided by the modification of DCT coefficient of chromatism in the YC_bC_r, the matrix of chromatism was disparted into 8×8 blocks, which were divided into two types by the density of edge point, then the watermark components with different strength were hided into the blocks according to the classification.

利用彩色图像在YCbCr域内对色差分量的DCT系数的改变加入水印,将色差分量矩阵进行8×8分块,根据每个小块内边缘点的密度将所有小块分成两类,不同类的小块分别按照不同的拉伸因子,加入水印信息。

And try to find out the effect of TSA on the proliferation and differentiation of rat tracheal stem cell. Methods 1.Preparation of 5-Fu Model and TSA treatment Two weeks" Wistar rats provided by the animal center of the China Medical University. The animals were sacrificed by 10% Chloral Hydrate 0.4ml/100g. Then the tracheas were excised aseptically. Then they were douched with PBS and cultured in 1:1 mix of Dulbecco"s modified Eagle"s medium and Ham"s F-12 medium (DMEM/F12) containing 120 mg/ml 5-Fu and 10% fetal bovine serum for 12h at 37°C. The tissues were then refreshed by culturing in DMEM/F12 containing 10% FBS and 200nM TSA.

1、制备气管损伤模型及剥离上皮取约200克左右的Wistar大鼠,雌雄不限(中国医科大学实验动物中心提供),腹腔注射10%水合氯醛0.4ml/100g,无菌条件下取出气管,无菌PBS反复冲洗,洗净血液和粘液,置于DMEM/F12培养液中(含10%胎牛血清),于培养液中加入终浓度为100mg/ml的5-Fu,37℃,5%CO2孵育12小时,弃去上述培养液,换成新鲜DMEM/F12液(含10%胎牛血清),同时加入终浓度为200nM的TSA继续培养,于换液后3、6、12、24、48小时分别10%中性福尔马林固定,石蜡包埋,制成4μm厚的组织切片。

That's where water treatment comes into the picture. A water treatment plant purifies the water by adding coagulant that precipitate impurities and particles in the water, allowing it to be flocculated and filtered. Chlorine is then added as a disinfectant before it can properly be called "tap water".

滤水厂将收集到的原水净化,一般都是将凝集剂加入原水中,使混杂於水中的悬浮物凝集成较大的粒块,再经过沉淀、过滤的净化过程,最后加入氯气消毒才能成为「滤水」。

The shortages of typical BP algorithm are that its convergence speed is slow and it possibly mires to local minimum. To solve the former shortage, BP algorithm was modified from several aspects, such as adjusting learning rate adaptively, adding momentum factor, preprocessing training data and modifying network structure, etc.. For the latter shortage, factor γ was introduced to avoid most of local minimum and accelerate the convergence speed.

为解决现有BP算法收敛速度慢的问题,对BP算法进行了改进,采用了动态改变步长、加入动量因子、对训练样本进行预处理以及调整网络结构等措施;针对经典BP算法可能收敛到局部极小点的问题,加入γ因子,可以避免大部分的局部极小值,从而使网络的收敛速度加快。

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Lao Qiu is the Chairman of China Qiuyang Translation Group and the head master of the Confucius School. He has committed himself to the research and promotion of the classics of China.

老秋先生为中国秋阳翻译集团的董事长和孔子商学院的院长,致力于国学的研究和推广。