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Thymocytes were also pretreated with or without ERKs, JNKs and p38 kinase inhibitors prior to UVB irradiation and DNA fragmentation was assessed by agarose gel electrophoresis. The activation of ERKs, JNKs, p38 kinase were investigated by western-blot.

透射电镜观察细胞超微结构的改变;琼脂糖凝胶电泳观察各组不加入或分别加入ERK抑制剂、JNK抑制剂和P38抑制剂后的DNA ladder;western-blot检测磷酸化ERK、磷酸化JNK、和磷酸化P38的活性。

NOVELTY - Alkali chemicals are added to the cyanogen group gold-containing liquid, to obtain an alkaline liquid. Zinc is added to the alkaline liquid, and the formed deposit is dissolved in aqua regia. A reducing agent is added to the deposit solution and a deposit is produced. Gold is then separated by heat-processing the deposit.

本发明的新颖性在于:将碱性化学物质添加到氰化含金液中,以获取碱液;将锌加入到碱液中,并将形成的沉积物溶解到王水中;然后在沉积溶液中加入一种还原剂,形成新的沉积物;最后通过加热处理沉积物来分离金。

The following day, Jackson performed to a sellout crowd of 40,000 at the Sydney Cricket Ground.

然后到了午夜,在一个私人房间的楼下,当这歌手加入到约30个伙伴的演唱会终点站派对时,身穿白衣化好妆的Rowe悄悄加入。

Methods The purified general bacteria in broth culture for46hourswerepreserved in glycerol-physiological saline,the purified streptococcus and Neisseria scraped from the agar culture were preserved in fresh sheep-blood which was added suitable glycerol-physiological saline,the purified fungi were cultivated in half-solid culture;-20℃general bacteria and fungi,-80℃streptococcus and Neisseria.

将普通细菌的菌种纯化后接种于普通肉汤管,4~6h增菌后,加入适量甘油-生理盐水保存液,-20℃冰箱保存;链球菌属和奈瑟氏菌属的菌株纯化后,用接种环取菌置于新鲜脱纤维绵羊血中,加入适量保存液,-80℃低温冰箱保存;真菌菌种纯化后接种于带硅胶塞的半固体培养基,25℃培养24h后,-20℃冰箱保存。

The goldsmith, who had a on his back, and —— like all hunchbacks —— was forward enough, stepped right up. The tailor was at first a little shy and held back, but as soon as he saw what fun it was, he too took heart and joined in.

那个金匠背上有个瘤,就像所有的驼背一样,他大胆地加入了跳舞者的圈子,而裁缝开始还有些害怕,想退缩,但他看到所有人都玩得那样开心,便也鼓起勇气加入了他们的行列。

The goldsmith, who had a hump on his back, and —— like all hunchbacks —— was forward enough, stepped right up. The tailor was at first a little shy and held back, but as soon as he saw what fun it was, he too took heart and joined in.

那个金匠背上有个瘤,就像所有的驼背一样,他大胆地加入了跳舞者的圈子,而裁缝开始还有些害怕,想退缩,但他看到所有人都玩得那样开心,便也鼓起勇气加入了他们的行列。

This article discussed mainly about the draft beer production technology with balsam pear as auxiliary materials in the meantime, discussed the elements in balsam pear and the juice making method.

本文主要探讨了在生产鲜啤酒过程中,加入一定比例的苦瓜汁参与发酵或在过滤前加入,研制出苦瓜鲜啤酒。并对苦瓜的成分、瓜汁的制备进行了论述。

In 2000 joined the keyboarder Halfface, followed by the guitarist Machine and Dementia aka the photographer Sandra Jensen (who developed the art direction of the band).

2000年,键盘手Halfface加入这个团体,紧随其后一位名叫Sandra Jensen的摄影师(他为乐队创造了一个全新的艺术方向)以痴呆吉他手的身份加入。

The mechanical testing indicates that alloying and solid-solution treatment can evidently enhance mechanical properties of ZM5 alloy,Ca and mischmetal additions refined the microstructure obviously.

拉伸试验表明,合金元素的加入和固溶处理明显改善了ZM5镁合金的常温力学性能,微量Ca和稀土元素的加入能够显著细化显微组织。

Later incorporated in the coastal areas of the fashion dish in the menu when they joined the red onion, pepper, curry and lettuce, the Andean until the arrival of immigrants, it was the last to join the amorphous boiled sweet potato and corn in the form of the stick.

后来在收编沿海地区的时尚菜谱时这道菜里又加入了红洋葱,辣椒,咖喱和莴苣,直到安第斯移民的到来,它才最后定形为加入煮红薯和玉米棒子的形式。

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