副枝

Western blot analysis shows that the protein could be identified only by the antiserum to M. bovis but not the sera against.M, paratuberculosis, brucellosis, babesia and infectious bovine rhinotracheitis virus.

Western blot分析显示：该融合蛋白能与抗牛分枝杆菌阳性血清发生特异性反应，而与牛副结核病、牛布氏杆菌病、牛巴贝斯病和牛传染性鼻气管炎病阳性血清不反应。

Leaves narrowly lanceolate to linear-lanceolate, base cuneate; plants glabrous; epicalyx segments shorter than to equaling sepals.

叶狭披针形的到线状披针形，基部楔形；植株无毛；为了等于萼片的副萼片短于。 2 L。 virgatum 帚枝千屈菜

Epicalyx segments deltate, abaxially keeled, slightly incurved in bud; branchlets and panicles densely gray puberulous; floral tubes 6- or 7-ribbed; ribs rounded or obscure.

在芽中的副萼片三角形，背面具脊，稍弯曲；浓密的小枝和圆锥花序灰色被微柔毛；花被管6-或7肋；棱圆形的或不明显。 6 L。 venusta 西双紫薇

The Vietnamese material is dearly distinguished from the examined holotype (AMNH 8365)and paratypes (AMNH 10316, 10307) of Schistura fasciolata by the pelvic-fin origin situated below unbranched or first branched dorsal-fin ray (vs. pelvic-fin origin dearly in front of dorsal-fin origin in S.fasciolata).

越南采集的标本和横纹南鳅的模式标本（AMNH 8365）和副模式标本（AMNH 10316， 10307）有明显的差别，腹鳍起点位于不分支鳍条或第一分背鳍枝鳍的下方（vs S.fasciolata的腹鳍起点明显在背鳍起点的前方）。

The Company's subsidiary, Designer Diagnostics, is engaged in the research and development of diagnostic test kits designed to be used for the rapid identification of infectious diseases such as Paratuberculosis and Mycobacterium avium-intracellulare.

该公司的附属公司，设计师诊断，从事研究和诊断医疗的发展要为传染病的快速识别使用，例如副结核病和结核分枝杆菌胞内。

The expression protein was analyzed by using the Western-blot, which proved that it had theantigenic activity of Mycobacterium paratuberculosis.

经免疫印迹分析证实，该融合蛋白具有副结核分枝杆菌抗原性。

Studies show the M. paratuberculosis secretes immunological competent protein in short termculture and opposites in long term culture.

研究表明，在短期培养过程中，副结核分枝杆菌分泌具有免疫活性的蛋白质，而在长期培养则相反。

The research results and expression product could serve as a basis for further studies on the gene-engineering vaccine, subunit vaccine and nucleonicacid vaccine against Bovine Paratuberculosis.

为进一步研究副结核分枝杆菌基因工程亚单位疫苗、核酸疫苗及诊断试剂奠定了基础。

Paratuberculosis;map0862 gene;map2154c gene;splicing overlapping extension;recombinant expression

禽分枝杆菌副结核亚种；map0862基因；map2154c基因；重叠延伸剪接技术；重组表达

In order to develop an indirect ELISA for diagnosing bovine paratuberculosis, the DNA fragmentsof map086 2and map2154c were amplified from the genome DNA Mycobacterium avium Subspeciesparatuberculosis K10. Then the DNA fragments of map0862 and map2154c were spliced byoverlapping extension, yielding a fusion gene map0

为了建立一种有效的牛副结核病间接ELISA方法，本研究设计了两对引物，从副结核分枝杆菌P_(18)株的基因组中PCR扩增出两个目的基因map0862和map2154c、，采用重叠延伸剪接技术(splice by overlapping extension，SOE)获得融合基因map0

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。