副枝

Inflorescences with a single cymule, rarely a second in unusually robust material, sometimes elongated and branched; corona lobes lanceolate to circular, flattened, gynostegium rounded to almost flat, not narrowed above outer corona lobes; corolla yellow

花序具一单的小聚伞花序，很少在异常粗壮的材料里的一秒，有时拉长和分枝；披针形的副花冠裂片对圆，扁平，圆形对几乎平，在上面不缩小外部副花冠裂片；花冠黄 6

Paratuberculosis (MAP,Mycobacterium paratuberculosis) induce a series of characteristics as chronic hyperplastic enteritis andprogressing extenuation in ruminants also called Johne\'s disease.

副结核病是由副结核分枝杆菌即禽分枝杆菌副结核亚种Mycobacterium avium subsp。

Bovine paratuberculosis is caused by Mycobacterium paratuberculosis and it is a kind ofchronic wasting disease in ruminants globally, which is called Johnes disease.

副结核病是由副结核分枝杆菌引起的一种全球性以反刍动物为主的慢性、消耗性传染病，也称Johnes。

Pasteurization, which is often used for milk or waste milk, can be used to reduce bacteria levels and has been shown to be very effective at killing a variety of pathogenic bacteria including Salmonella, E. coli, Mycobacterium avium subspecies paratuberculosis, Mycobacterium californicum, Mycobacterium bovis, and Listeria monocytogenes.

巴氏灭菌常使用于牛奶或牛奶废料，它可以用来降低细菌水平，并已被证明能有效的杀死多种致病细菌，其中包括沙门氏菌、大肠杆菌、副结核分枝杆菌亚种、californicum分枝杆菌、牛分枝杆菌和李斯特菌。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

Cymes mainly on branched axis, 3 or 4 × dichotomously branched, branches slender and patent; peduncle 1-2 cm, secondary peduncle 5-15 mm; pedicels very short, less than ca. 3 mm or absent.

聚伞花序主要在分枝的轴上，3或者4二歧分枝，分枝纤细和开展；花序梗1-2厘米，副花梗5-15毫米；非常短的花梗，少于长约3毫米或无。

The reaction mechanism, by-reaction inhibition, effect of initiator, and monomer on grafted products are studied extensively. Polyethylene grafted with maleic anhydride, as the hot melt adhesive for the aluminum-plastics compound pipe, is also studied as a goal for engineering application.

对反应挤出接枝体系进行了热分析，确定了合理的反应挤出工艺条件：用红外光谱和化学滴定的方法分别对接枝结构和接枝率作了定性表征和定量测定；对三种反应挤出体系的反应机理和副反应的抑制，以及引发剂、单体对接枝产物的影响进行了深入系统地研究；对PE接枝MAH作为铝塑复合管的热熔胶进行了工程化应用研究。

The suitable reaction conditions are determined by thermal analysis for grafting systems via reactive extrusion technology. The grafted structure and grafted ratio are determined and tested by infrared spectrometry and chemical tritration method. The reaction mechanism, by-reaction inhibition, effect of initiator, and monomer on grafted products are studied extensively. Polyethylene grafted with maleic anhydride, as the hot melt adhesive for the aluminum-plastics compound pipe, is also studied as a goal for engineering application.

对反应挤出接枝体系进行了热分析，确定了合理的反应挤出工艺条件：用红外光谱和化学滴定的方法分别对接枝结构和接枝率作了定性表征和定量测定；对三种反应挤出体系的反应机理和副反应的抑制，以及引发剂、单体对接枝产物的影响进行了深入系统地研究；对PE接枝MAH作为铝塑复合管的热熔胶进行了工程化应用研究。

The suitable reaction conditions are determined by thermal analysis for grafting systems via reactive extrusion technology. The grafted structure and grafted ratio are determined and tested by infrared spectrometry and chemical tritration method. The reaction mechanism, by-reaction inhibition, effect of initiator, and monomer on grafted products are studied extensively.

对反应挤出接枝体系进行了热分析，确定了合理的反应挤出工艺条件；用红外光谱和化学滴定的方法分别对接枝结构和接枝率作了定性表征和定量测定；对三种反应挤出体系的反应机理和副反应的抑制，以及引发剂、单体对接枝产物的影响进行了深入系统地研究；对PE接枝MAH作为铝塑复合管的热熔胶进行了工程化应用研究。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。