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One night the big bad wolf, who dearly loved to eat fat little piggies, came along and saw the first little pig in his house of straw. He said "Let me in, Let me in, little pig or I'll huff and I'll puff and I'll blow your house in!""Not by the hair of my chinny chin chin", said the little pig. But of course the wolf did blow the house in and ate the first little pig.

一天晚上,一只早就想吃胖小猪的大灰狼,来到第一只小猪用稻草建的房子前,他说,"让我进去,让我进去小猪,不然我就吹掉你的房子""不要废话"小猪说但是狼真的吹破了房子,吃掉了第一只小猪。

The DNA replication is usually assayed by denatured gel electrophoresis and autoradiography, which are complex, time-consuming, and incapable of acquiring the dynamic data in real time.

目前DNA复制的研究,一般采用放射性标记与凝胶电泳相结合的方法,操作复杂,不利于方便快捷地捕获生命活动过程中的重要信息。

Chronic renal failure; with pyonephrosis; diagnosis; treatment

慢性肾功能衰竭;肾积脓;诊断;治疗