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After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株猪嗜血杆菌血清5型地方株为研究对象,提取细菌基因组DNA,用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切,回收大小为1~5Kb的DNA片段,将其连接入原核表达载体pET-28a-c,最后转化宿主菌,结果成功地构建了基因表达文库,为后续的基因筛选工作奠定基础;另外,本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计,以猪嗜血杆菌血清5型地方菌株的基因组DNA为模板,进行PCR扩增反应,结果表明成功地获得两个新基因:23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸),并进一步做了不同物种之间的分子系统发育分析,构建了基于23S rRNA基因的邻接法系统发育树,阐明了猪嗜血杆菌与其它菌种的分子进化关系。

For those in control group (n=14), the animals did not undergo any surgery of the nerve fibers and ganglia. In Vi group (n=9), the animals underwent bilateral resection of the nasociliary and postganglionic parasympathetic nerve fibbers. In SCG group (n=8), the animals underwent bilateral resection of supper cervical ganglia. In V1+SCG group (n=9), the animals underwent both surgeries as Vi and SCG group did. In sham operation group (n=11), the animals underwent carotid manipulation with blunt-tipped forceps as well as dissection of nasociliary and postganglionic parasympathetic nerve fibers around the ethmoidal foramen, without cutting any nerves. SCS was performed on rats and CBF was monitored by laser Doppler flowmeter recordings.

SD大鼠分5组:对照组(n=14,不对神经纤维和神经节作任何手术处理)、V1组(n=9,切断双侧鼻睫状神经及其交感节后纤维)、SCG组(n=8,切断双侧颈上神经节)、V1+SCG组(n=9,切断双侧鼻睫状神经及其交感节后纤维和双侧颈上神经节)、假手术组(n=11,手术暴露双侧鼻睫状神经及其交感节后纤维和双侧颈上神经节,但不切断神经纤维或神经节);给予大鼠SCS,并用激光多普勒实时记录脑血流。

The unit of radioreaction in ethmoidal sinus was ethmoidal cell. Several ethmoidal sinus cells were fused to patch in severe cases. The radioreactions in ethmoidal sinus, maxillary sinus and sphenoidal sinus were seen within three months, after three months and after one year respectively. And nasal sinus radioreactions of most cases were not absorbed spontaneously after one year. When the two kinds of radiotherapies were equal in dosing level, there was no obvious difference between them in respect to the radioreaction of accessory sinus, but when the dosing level was above 3182GBq, the radioreaction of accessory sinus was remarkably increased, compared with those caused by lower-dose radiotherapies.

筛窦放射反应以筛窦小房为单位,重者也可以多个小房融合成片状;筛窦的放射反应在放疗后3个月内即可出现,上颌窦常在3个月以后出现放射反应,而蝶窦一般在放疗后1年以后才出现;大多数病例鼻窦放射反应在1年以后难以自动吸收、消退;两种方法放疗剂量相同时所致鼻窦放射反应无明显差别,但放疗剂量在3182 GBq以上时所致鼻窦放射反应较其它放疗剂量时明显增高。

Control group, the animal underwent SCS and LDF recordings without any surgery of the nerve fibers and ganglia. V1 group, the animal underwent bilateral resection of the nasociliary and post-ganglionic parasympathetic nerve fibbers. SCG group, the animal underwent bilateral resection of supper cervical ganglion. V1 + SCG group, the animal underwent both surgeries as V1- and SCG-group animals did. Sham group, the animal underwent the carotid manipulation with blunt-tipped forceps as well as the dissection of nasociliary and post-ganglionic parasympathetic nerve fibers around the ethmoidal foramen, but without cutting any nerves.

动物分成5组,对照组:不对神经纤维和神经节作任何手术处理;V1组:切断双侧鼻睫状神经及其交感节后纤维;SCS组:切断双侧颈上神经节;V1+SCG:切断双侧鼻睫状神经及其交感节后纤维及双侧颈上神经节;假手术组:手术暴露双侧鼻睫状神经和其交感节后纤维及双侧颈上神经节,但不切断神经纤维或神经节。

Provincial government economy consultants such as Brennan , the CEO of Britain AstraZeneca, Sanmu-Fanguang, the president of the Bank of Tokyo-Mitsubishi UFJ, Ltd., Du Zherui, the president and Management Committee Chairman of the French Carrefour Group, Fan Dawei, the chairman, president and CEO of Emerson Electric Company, Uichiro Niwa, the chairman of the Itochu Corporation, Ding Zhaozhong, the professor of Massachusetts Institute of Technology and Nobel laureate, Chen Yongzheng, the senior vice president and Greater China CEO of Microsoft, Ke Zilei, the president and CEO of Netherlands Royal Philips Electronics, Tingtian-Shengyan, the president of Japanese Sharp Corporation, Wu Hele, the member of the Executive Committee of German Siemens AG, Liu Zunyi, the president of the Chinese University of Hong Kong, Yang Lieguo, the Chairman of Singapore Standards, Productivity and Innovation Board, Mude, the president of the University of Maryland, Bei Songning, the president and CEO of UPM Group,4 consultant representatives like Luo Ruizhe, the vice president of Lucent Alcatel's global executive and the president of Asia Pacific region, Bo mule the member of Germany's BASF Corp. Executive Board, Cai Beishan, the vice global executive president of Swedish Ericsson Group, Zeng Shisheng, the President Assistant of Singapore Keppel Ltd, have separately given the speech on "the developmental momentum of China's pharmaceutical industry ", and put forward many unique insights and ideas from different angles, different levels, and different areas, as well as the positive and constructive opinions and suggestions.

英国阿斯利康公司首席执行官布伦南,日本三菱东京日联银行股份有限公司会长三木繁光,法国家乐福集团总裁兼管理委员会主席杜哲睿,美国艾默生电气公司董事长、首席执行官兼总裁范大为,日本伊藤忠商事株式会社会长丹羽宇一郎,美国麻省理工学院教授、诺贝尔奖获得者丁肇中,美国微软公司资深总裁、大中华区首席执行官陈永正,荷兰皇家飞利浦电子集团总裁兼首席执行官柯慈雷,日本夏普株式会社会长町田胜彦,德国西门子股份公司执行委员会成员吴贺乐,香港中文大学校长刘遵义,新加坡标准、生产力与创新局主席杨烈国,美国马里兰大学校长牟德,芬兰芬欧汇川集团总裁兼首席执行官贝松宁等14名省政府经济顾问,阿尔卡特朗讯公司全球执行总裁兼亚太区总裁罗睿哲,德国巴斯夫股份有限公司执行董事会成员薄睦乐,瑞典爱立信集团公司全球执行总裁北山,新加坡吉宝企业有限公司董事长助理曾士生等4名顾问代表,分别在会上就&中国制药行业的发展动力&等作了专题演讲,从不同角度、不同层面、不同领域提出了许多独到的见解和理念,提出了积极而富有建设性的意见建议。

Mm,humeroradial joint cavity 0.69±0.44 mm, proximal radioulnar joint cavity 0.90±0.56 mm, thethickness of joint cartilage (joint cartilage of humerus 1.15±0.35 mm, joint cartilage of ulna 1.04±0.16 mm, joint cartilage of radius 0.98±0. 17 mm) and the collateral ligament (thickness ofthe ulnar collateral ligament 1.38±0.42 mm, thickness of the radial collateral ligament 1.32±0.42 mm) were all measured.

结果:肱尺关节腔为0.70±0.44 mm,肱桡关节腔为0.69±0.44 mm,桡尺近侧关节腔为0.90±0.56 mm;关节软骨厚度,骨关节软骨厚度为1.15±0.35 mm,尺骨关节软骨厚度为1.04±0.16 mm,桡骨关节软骨厚度为0.98±0.17mm;侧韧带:尺侧韧带厚度为1.38±0.42mm,桡侧韧带厚度为1.32±0.42mm。

To assess the thermal contribution from oxidation reaction, the standard formation enthalpes of all reactants and products were used to calculate the oxidation enthalpies. It could be found that all oxidation reactions were exothermic. And oxidation played a more important role during the toluene trinitration at high temperature, because the reaction temperature of toluene trinitration is the higher than mononitration and dinitration, and the temperature coefficient of oxidation is higher than that of trinitration too.

为了考察不同阶段氧化反应对反应放热的贡献,用反应物和产物的标准生成焓推得氧化反应的理论反应热,计算结果表明,各段硝化的氧化反应均为放热反应,其中三段硝化反应由于反应温度最高,氧化反应的温度系数比硝化反应高,故氧化反应对三段硝化影响最大。

Pereira's attitude towards the Jesuit China Mission has been described by Western historiography as an obstinate defense of the interests of the Portuguese Padroado, something that can not be denied. However, he was neither alone ― his was a position followed also by several non-Portuguese Jesuits ― nor an extremist: after all, he was just a follower of the founding universalistic principles and ideals of the Society of Jesus.

徐日升对&礼仪之争&、中文礼拜、本地神职人员及传教士的招募,更不用说他对教会组织、等级依赖、后勤支持、教堂的设立及生存等方面的看法,研究得十分贫乏,尽管他在耶稣会身居要职,如北京学院院长(1688–1691)、北京地区巡视员(1687–1688,1691–1692)、中国会长(1692年6月29日至1695年6月29日)。

METHODS: Frozen fresh knees of cadavers were dissected gradually. Structural composition of MCL was observed, and the length and width of superficial and deep layers of the MCL were measured. The position, shape, size of the attachment points and their relationships were observed, and the tensity of the superficial and deep layers of MCL and posteromedial articular capsule were observed during flexion and extension of the knee, from which their functions were deduced.

冰冻新鲜尸体标本膝关节进行逐层解剖,观察内侧韧带结构组成:测量内侧韧带浅层和深层的长度、宽度;观察它们附着点的位置、形状、大小以及相互关系,以及观察内侧韧带浅层与深层及后内侧关节囊在屈伸膝关节时的紧张度,由此推测其功能。

Sybilla:A woman in my place has two faces; one for the world, and one which she wears in private. With you I'll be only Sibylla.

茜贝拉:在我这个位置的女人有两面孔;一展示给世人,一是私底下所显现,对你而言我只是茜贝拉。。。

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