副

"Our protocol represents a baseline for the amount of calcium that a given patient will require following successful parathyroidectomy for the treatment of primary hyperparathyroidism," she told Medscape General Surgery.

她向Medscape外科学表示，我们的方案代表成功接受副甲状腺切除治疗原发性副甲状腺功能亢进的患者，所需要的钙补充基准量。

Parathyroidectomy remains the only definitive therapy for this disease. The development of minimally invasive radioguided parathyroidectomy has enabled a quick and safe approach to this therapy.

在治疗方面，副甲状腺切除手术是目前唯一能有效根治的方法，其中微创放射导引副甲状腺切除手术(Minimally invasive radioguided parathy-roidectomy)的发展则可有效缩短手术时间及其并发症。

We concluded that serum calcium should be screened in all urolithiasis patients, and parathyroidectomy will offer greatest satisfaction in treat-ing primary hyperparathyroidism.J Urol

我们认为所有尿路结石病患皆应接受血清钙筛检，因为一旦诊断是原发性副甲状腺功能亢进症，副甲状腺切除术将可获致极佳之疗效。

Bovine paratuberculosis is caused by Mycobacterium paratuberculosis and it is a kind ofchronic wasting disease in ruminants globally, which is called Johnes disease.

副结核病是由副结核分枝杆菌引起的一种全球性以反刍动物为主的慢性、消耗性传染病，也称Johnes。

Sera from 90 cattle with bovine paratuberculosis and 100 cattle without bovine paratuberculosiswere tested.

通过对90份牛副结核病血清和100份无副结核病牛血清的检测结果表明，ELISA方法的敏感性为84.44％(76／90)，特异性为97％(97／100)。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

In order to develop an indirect ELISA for diagnosing bovine paratuberculosis, the DNA fragmentsof map086 2and map2154c were amplified from the genome DNA Mycobacterium avium Subspeciesparatuberculosis K10. Then the DNA fragments of map0862 and map2154c were spliced byoverlapping extension, yielding a fusion gene map0

为了建立一种有效的牛副结核病间接ELISA方法，本研究设计了两对引物，从副结核分枝杆菌P_(18)株的基因组中PCR扩增出两个目的基因map0862和map2154c、，采用重叠延伸剪接技术(splice by overlapping extension，SOE)获得融合基因map0

The plant Quarantine Lab had intercepted more than 200 quarantine pests such as : Tilletia indica , Tilletia controversa , Bursaphelenchus xylophilus , sorghum halepense , Acanthoscelides obtectus , Sternochetes mangiferae , Bactrocera dorsalis , Coptoermes curvignathus for nearly 5000 batches ; The Animal Quanantine Lab had intercepted about 20 cow which had some pathogens evidence of infectious disease such as : bovine infectiouse rhinotracheitis , leukaemia bovum , paratuberculosis and so on ; The food microorganism lab had detected over 300 unqualified products such as Salm-Sury , Listeria monocytogenes , Mycobacterium paratuberculosis etc .

多年来，分中心各实验室切实履行严格把关的职责，植物检疫共截获大豆疫霉病菌、小麦印度腥黑穗病菌、小麦矮腥黑穗病菌、松材线虫、假高梁、菜豆象、芒果果核象甲、桔小实蝇、大家白蚁等国家禁止进境的危险性有害生物220 多种近10000 批次；动物检疫发现感染牛传染性鼻气管炎、牛白血病、副结核等传染病的疫牛20 多头；食品微生物检测共检测沙门、单增、副结核的不合格商品300 多批；分子生物学实验室为国内企业出具非转基因证书和不含有牛羊源成分证书50 余份，使相关的产品能够顺利出口到欧盟、韩国和日本等国。

Tzu Chi's care is boundless. So, in Sao Miguel Paulista, the stationery students receive is a New Year's treat! On top of that, volunteers return to offer medical service and give these people a pair of glasses, so they can enjoy the world with greater clarity.

慈济的爱无界线，在圣米桥保力司达也能看到，文具是志工送学童的新年礼物，不仅如此蓝天白云的志工，甚至延续上回的义诊服务，把爱化作一副副眼镜，希望这儿的居民因为善心，人生旅程能一帆风顺。

Based on the analysis of Sea Surface Height Anomaly from satellite observation, it is proved that the oscillation with period of 70～210 days is dominating in two branches of the eastward countercurrent in Subtropical North Pacific: Subtropical Countercurrent and Hawaiian Lee Countercurrent.

根据对卫星观测的海平面高度资料的分析，进一步证实了在北太平洋副热带两支向东的流（副热带逆流和夏威夷背风逆流）所在的区域内，海平面高度的70～210天周期振荡是主要的低频变化。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。