剪接

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

Hepatitis B virus is the smallest DNA virus which is a member of the viral family Hepadnavirida. It uses its DNA as template and the RNA polymerase II of the host cells to transcribe its viral transcripts. There are four major RNAs transcribed from HBV genome. All of the four major transcripts are unspliced, terminated at a common site, polyadenylated at the 3' terminal and capped at the 5' terminal.

B 型肝炎病毒属於肝病毒科，为目前已知最小的去氧核醣核酸病毒，利用其病毒的去氧核醣核酸以及宿主细胞内的核醣核酸聚合酶可转录出四种主要的核醣核酸产物，此四种主要的转录产物均未经剪接并终止於核心开放编阅序列的共同polyadenylation 讯号，且在其5'端均经过capping修饰。

Comparison of the mbC cDNA sequences indicated the presence of multiple variants of this α1 isoform.

单一的转录产物在小鼠脑中被替换剪接而产生了这些异构体。

For comparison, a legit image from March 27, 2009 3:18 showed now such signs of smearing or cut-and-paste.

一个2009年3月27日 3：18分的合法影像科可作为比较，显示了其涂改，剪接和粘贴的迹象。

Analysis of EST revealed there are probable alternative splices at its 5 end.

对EST数据的分析结果表明在其5'端可能存在选择性剪接。

Conserved motifs found in each cluster were searched against factor database and most of them were functional.

这些保守短序列很有可能在剪接位点的选择过程中发挥重要的作用。

In this study, 105 TDFs were in silico mapped in the rice high-density linkage map. Nineteen TDFs were mapped to the quantitative trait loci regions for root growth under water-limited conditions in, at least, two of three rice populations derived from three crosses with the same parent of Azucena (Bala×Azucena, IR64×Azucena and IR1552×Azucena). Four of 19 genes (T37, L16, T17 and T7) were mapped based a RIL population of IR1552×Azucena by southern blot analysis. Five genes encode putative or hypothetical protein. Other 14 genes were similar with known genes in databases including expansin (OsEXP2), late embryogenesis-abundant gene , an SR-related protein essential for spliceosome assembly (SART1), autophagocytosis protein , bHLH protein, fruit-ripening protein similar to ASR, nickel-binding protein 2A, DNA-binding protein, pyruvate dehydrogenase kinase , stomatin-like protein, SR1 induced by sucrose starvation, vacuolar protein sorting protein (VSP33a), gibberellin action negative regulator and retroelement.

根据核苷酸序列将105个基因电子定位到水稻的高密度连锁图谱上，其中19个差异表达基因定位在Bala×Azucena、IR64×Azucena和IR1552×Azucena中至少两个群体共同的与根生长相关的QTLs区间，并用Southern杂交将其中的4个定位到IR1552×Azucena群体遗传连锁图谱相应的位点上。19个基因中的5个编码推断的未知功能蛋白质，其余14个编码已知功能蛋白，分别为膨胀素（Os-EXP2）、胚胎后期丰富蛋白、剪接体安装必需的SR相关蛋白（SART1）、自吞噬蛋白、碱性螺旋-环-螺旋转录因子、与ASR相似的果实成熟蛋白、镍结合蛋白、DNA结合蛋白、丙酮酸脱氢酶激酶、stomatins类蛋白、蔗糖调节蛋白SR1、液泡蛋白分类蛋白（VSP33a）、GA负调节因子、逆转座元件。

He used in hand's coffee cup to bump the narrow-mouthed bottle, swayed the film editing exited from mine hand caper.

他用手上的咖啡杯碰了碰细口瓶，晃荡的剪接就从我的手跳跃出去。

This model is proved to be particularly suitable for modeling the biological structure of acceptors.

隐半马氏模型能够很好地刻画3'剪接位点的结构。

In the study, RT-PCR and sequencing were employed to detect the expression levels of CCK-B receptor, CCKBRi4sv and gastrin mRNAs in 30 human gastric carcinoma and their corresponding normal tissues, 10 gastritis and 2 autopsied normal stomach specimens as well as in a gastric carcinoma cell line SGC-7901 cells.

本研究首先用RT-PCR及测序方法检测了30例人类胃癌组织及癌周正常胃粘膜、10例胃炎、2例尸检胃组织及胃癌细胞株SGC-7901中CCK-B受体、CCK-B受体的异位剪接变异体(intron 4-contained variant of cholecystokinin B receptor，CCKBRi4sv)及胃泌素基因的表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。