剪接

These analyses disclose the potential components influencing the exon splicing frequency, and provide the clue for further decipherment of novel ASEs creation, splicing selection and transcription regulation.

这些分析结果揭示了影响外显子剪接频率的潜在组成特征，也为进一步解读新的选择性剪接外显子的生成、剪接选择和转录调控提供了线索。

Finally, this thesis presents an initial study on the splicing and alternative splicing mechanism of eukaryotic genes.

最后，我们对真核基因的剪接机制，以及选择性剪接的机制进行了初步的研究。

However,there are a lot of problems unsettled by now in this field,such as the low efficiency of splicing in heterologous hosts,the low yield of intein mediated purification system and the impracticability of artificial control on the process of splicing.

但是，在目前的研究中，仍然存在着大量未解决的问题，如异源生物体内剪接效率低、蛋白质内含子纯化系统效率低以及不能实现蛋白质剪接人为控制等。

Point mutation and additional sequence insertion in introns might have given rise to new isoforms.

对比usf1和usf2的各个选择性剪接变体发现，二者的剪接模式都很保守，而新的变体是由点突变或内含子中额外序列的插入造成的。

The relationship between the genetic deficiencies and hemorrhagic disorder were characterized. The homozygosity of the Thr359Met in the propositus with FⅦ: C activity 2% was related to severe clinical symptoms; The proband with double heterozygous lesions of Arg152Leu and one single nucleotide deletion at position 11487-9, combined with Arg304Trp in exon 8 with FⅦ activity 1% suffered severe clinical bleeding tendency. one proband with double heterozygous mutations (Agr304Trp and Arg304Gln) with 10% of FⅦ activities characterized asymptom. the heterozygous mutations (Thr359Met, Arg152Gln,-55C→T) with FⅦ: C 1%～5. 5% showed moderate or mild clinical symptoms.

其中8961 G＞T（Arg152Leu）和10966-8delC两种突变为国际首次报道，IFSla+5g＞a突变导致的异常剪接为国际首次报道。1个F7及F10基因联合缺陷家系，其中，F10基因28139 G＞T（Val384Phe）突变为国际首次报道；发现了1个由F7及组织因子(tissue factor，TF)两种基因联合缺陷导致的出血家系，其突变分别为F7基因启动子区-55C＞T杂合突变；TF 9363 C＞T（Arg131Trp）杂合多态性（频率2.63％）。6种F7基因突变发生在催化区；2种突变发生在裂解位点；1种发生在启动子区；1种突变发生在剪接位点，除一种缺失突变外，其余均为点突变；所有家系的基因突变都来自先证者的父亲和/或母亲。

These data suggest that increasing the turnover rate of G6pt by alternative splicing may induced through a common MyoD-dependent mechanism during myogenesis of muscle cells. The alternative splicing of vG6pt is unstable and has higher turnover rate during myogenesis.

由这些实验结果得知，G6pt的另类剪接能藉著MyoD的相关机制而被诱发，而此另类剪接所产生较不稳定的vG6pt使在肌肉组织中有较高的汰换速率。

Additionally XBP1 mRNA which functioned as centrally regulating molecule during ER stress was spliced partially,while it was completely spliced in positive control cells and unspliced in H7721.These results gave the proof that blocking expression of GnT-V caused H7721"s ER stress and response was more weak than that caused by DTT. It may be the chronic process.Additionally,previous studies reported that the ds-RNA may activate the kinase PKR which phosphorylated eIF2 α and inhibited synthesis of proteins.In this paper the antisense cDNA of GnT-V was integrated with GnT-V-AS/H7721"s gene and functioned through the binding of antisense RNA and mRNA of GnT-V.Additionally,the integration of GnT-V cDNA in genome also may be a non-specific factor.

对这三种ER stress关键分子的检测发现：和阴性细胞相比较，实验细胞中BIP的表达无论是蛋白水平还是转录水平都有明显的上调，但上调程度都要低于DTT处理的ER stress阳性细胞；XBP-1 mRNA在实验细胞中部分被剪接，在阳性细胞中XBP-1 mRNA完全被剪接，而在阴性细胞中其以非剪接形态存在；此外和DTT处理的ER stress阳性细胞相似，实验细胞中的PERK发生磷酸化，表明ER stress过程中通过磷酸化eIF2α抑制蛋白合成机制的活化，这和芯片所检测到的GnT-V-AS／H7721细胞蛋白合成系统水平下调相一致。

The results showed that the ZNF191fu and ZNF434fu functioned as transcription repressors while the ZNF396fu can only weakly suppress the transcription activity of the reporter, and the ZNF397fu and ZNF447fu showed no signanificant capability of transcription regulation.

而转录因子发挥作用需要进入到细胞核中，因此，我们进一步分析了这些基因的不同剪接本在细胞内定位的情况，结果显示我们所检测的5个成员的nf剪接本都能被相应的fu剪接本带入细胞核内，并且这种作用是通过SCAN结构域所介导的。

And ZNF397nf function as transcription repressors. The different transcriptional regulation activity of the nf isoforms and their interaction with the fu isoforms would contribute to the complexity of the transcription regulation. To further examine the biological function of interaction between different isoforms we also analyze the potential role of ZNF191 and ZNF434 on signaling pathway. We found that ZNF191fu can significantly enhance the HSE-luciferase activity, while ZNF434fu can enhance the AP1-luciferase activity, then we analyze whether the nf isoforms have impact on the fu isoforms.

而这些成员的nf剪接本与它们对应的fu剪接本的转录调控功能有所不同，其中ZNF191nf与ZNF434nf剪接本的转录调控活性相对它们的fu剪接本显著降低，呈现较弱的转录抑制作用，但ZNF447nf剪接本却呈现出显著的转录激活作用，同时ZNF397nf剪接本则表现出显著的转录抑制作用，ZNF396nf剪接本的转录调控作用与其fu剪接本相当。nf剪接本这种与fu剪接本不尽相同的转录调控活性以及能与fu剪接本互作的能力，大大增加了转录调控作用的复杂性，这使得通过少量的转录因子作用来实现众多基因时空表达的不同成为可能。

Regulating mechanisms, influencing factors, specificities and function of alternative splicing in plant.

管如此，仍在选择性剪接的调控机制、剪接特异性和影响因素及剪接功能等方面取得了一定的进展。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了