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The results indicate that the STR method could be used in genetic monitoring of special inbred Xiphophorus helleri.

这些STR位点能够区分剑尾鱼三个近交系品系。结论 STR检测技术可望用于近交系剑尾鱼的遗传质量监测。

The HSP70 gene expression was determined in hepatopancreas, spleen, heart and head kidney in green swordtail Xiphophorus helleri challenged by Vibrio alginolyticus 0, 2, 5, 8 and 11 days after administration using RT-PCR. Results showed that tile gene expression of the HSP70 was of tissue specific.

用溶藻弧菌Vibrio alginolyticus感染剑尾鱼Xiphophorus helleri,取感染后第0、2、5、8、11 d的活鱼及感染后第2d濒死鱼,采用半定量RT-PCR方法分别检测HSP70基因在肝胰脏、脾脏、头肾和心脏组织中的表达。

Helleri RR-B strain and the average homozygote rate reached 100%. These loci could be used as markers in genetic monitoring of X. helleri RR-B strain, which had obtained fairly high genetic homozygosity by inbreeding for more than 20 generations.

结论]18个微卫星座位可作为剑尾鱼RR-B系遗传监测的分子标记;剑尾鱼RR-B系经20多代的近交培育已具有很高的遗传均一性。

The total RNA was extracted from spleen of Xiphophorus helleri which had been immunized for two weeks. The core sequence was amplified by RT-PCR. The cDNA end sequences were amplified by RACE, and then the full-length cDNA of sIgM heavy chains gene were obtained by putting all of them. The full-length sequence contains the immunoglobulin signature sequence: FKCIANH.

以免疫两周的剑尾鱼为材料,提取脾脏总RNA,通过逆转录-聚合酶链式反应扩增出分泌型免疫球蛋白M(secretory immunoglobulin M, sIgM)重链基因的核心序列,再应用3'和5'快速扩增cDNA末端方法扩增其末端序列,拼接后获得剑尾鱼sIgM重链全长cDNA序列。

This paper is an attempt to analyze the nutritional requirements and current diets, in order to promote further study of nutritional requirements and establishment of nutritional standard for laboratory zebrafish and swordtail.

本文就斑马鱼、剑尾鱼的营养需求及饲料现状进行了分析,以期能为斑马鱼、剑尾鱼营养需求方面更深入的研究及营养标准的制定有所帮助。

However, different types of tropical fish, the water temperature requirements are different, such as the guppy, swordtail can put up with around 10 ℃ low temperature, Discus fish, tiger fish, will be below 18 ℃, when the death.

但不同种类的热带鱼,对水温的要求是有差异的,如孔雀鱼、剑尾鱼可以忍耐10℃左右的低温,七彩神仙鱼、虎皮鱼等在低于18℃时便会死亡。

The results also showed that swordtail fish was more sensitive to these pollutant, and the result with swordtail fish had better reproduplication, so swordtail fish was a kind of good laboratory animals for aquatic toxic test.

在3个营养级别的受试生物中,剑尾鱼对上述毒物具有较好的敏感性,结果稳定,重现性好,说明剑尾鱼是一种优良的水生毒性试验材料。

The joint toxicity of two toxicants to swordtail fish was synergistic in 24h and antagonistic in 48h and 96h, both were treated as ratio 1∶1 of toxi...

剑尾鱼对Hg和Se的最大容许浓度分别为:0.0084和0.064 mg/L。结果还表明:剑尾鱼幼体对Hg和Se敏感,是一种较理想的环境污染指示生物。

Results showed LC_(50) of divalent mercury to swordtail fish were 0.84, 1.35, 1.71mg/L in 24h, 48h and 96h respectively, while LC_(50) of tetravalent selenium to swordtail fish were 6.64, 12.59, 17.91mg/L respectively.

结果表明:Hg+2对剑尾鱼的 24、48、96 h的 LC50分别为 1.71、1.35、0.84mg/L;Se+4对剑尾鱼24、48、96 h的Lc50分别为17.91、12.59、6.64 mg/L;在浓度和毒性比都为1∶1的条件下,24 h都表现联合作用,而48 h、96 h则为拮抗作用。

Livebearing toothcarps, such as guppies, platies, black mollies and swordtails also eat algae.

卵胎生鳉科鱼类,例如孔雀鱼、月光鱼、黑摩丽和剑尾鱼也吃食藻类。

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