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RESULTS:In vertebral pulp extirpation group, the stress was proved to be the highest at superior edge, posterior middle part, lower edge and anterior middle part of small joints under anteflexion, backward extension, compression, lateroflexion and revolving states, moreover, small joint stress in artificial lumbar intervertebral disk was higher than that in normal intervertebral disk, but obviously lower than that in vertebral pulp extirpation group;however, the small joint of the middle part of artificial lumbar intervertebral disk bore the highest stress under revolving states.

结果:髓核摘除组小关节的上边缘部、后中部、下边缘部和前中部在前屈、后伸、压缩、侧屈、旋转状态应力最大,人工腰椎间盘组的小关节的应力比正常椎间盘高,但明显小于髓核摘除组,但正中部在旋转状态下,人工腰椎间盘小关节的中心部位承受的应力最大。

Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数按摩明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。

Vestibule; temporal bone; bony labyrinth; inner ear; perilymph; angular acceleration; hair cells; stereocilia; crista ampullaris; ampulla; cristae; cupula; vertigo; VOR; vestibulo-ocular reflex; vestibular nerve; vestibular nuclei; vestibular ganglion; equilibrium

前庭;颞骨;骨迷路;内耳;外淋巴;角加速度毛细胞;静纤毛;壶腹嵴;壶腹;嵴;吸盘;眩晕;VOR;前庭眼球反射;前庭神经;前庭神经核;前庭神经节;平衡

Double-labeled neurons were also observed in the raphe nucleus, the lateral paragigantocellular nucleus, and the vestibular nucleus.

另外, 在中缝大核、旁巨细胞外侧核及前庭神经核也观察到双标神经元。

The double labelling technique by combination of horseradish peroxidaseretrograde tracing with immunohistochemical staining for γ-amino-butyric acidwas used to observe the distribution of GABA-positive neurons in the abducens and vestibular nucleus,and its projection to oculomotor nucleus of rats.

用辣根过氧化物酶(horseradish peroxidase,HRP)逆行标记结合γ-氨基丁酸(γ-amino-butyric acid,GABA)的免疫组织化学双标技术观察大鼠的展神经核和前庭神经核内GABA阳性神经元的分布,以及其向动眼神经核的投射。

It is the first time that these phenomena were observed: Two nuclei are surrounded by plasma and locate at the central of the female gametophyte and the others nuclei are positioned at the fringe of the female gametophyte; Before the pollen tube enters the female gametophyte. the nuclei in chalazal end begin splitting of plasma and form multinuclear cell.4. The pollens of Gnetumc are spherical or applanate with single aperture. The ornamentation of exine is spine. The basis part of spine is lenience and the top part of spine is tip or obtuse sphere.

首次在买麻藤属植物的雌配子体中观察到2个游离核位于配子体的中央位置,且被一团原生质所包围的,其余的游离核位于边缘的现象以及花粉管进入雌配子体前,合点端的核已经发生胞质分裂形成多核细胞的现象 4、买麻藤的花粉近球形或扁平型,有单萌发孔,外壁表面具小刺状纹饰,小刺基部宽大,末端尖或钝圆。

The proliferation of SGC-7901 cell line was markedly inhibited by shRNA-GC-CⅢ( P .05). There was changes in cell morphology, such as cell volume reduction, karyopyknosis and chromatin conglomeration. FCM showed that GC-C inhibition caused the appearance of sub-G 0 /G 1 peak which increased from 5.47%(48h after transfection) to 5.63%(72h after transfection).

转染GC-C shRNA真核表达载体后SGC-7901细胞的增殖能力受到抑制( P 。05);细胞形态发生改变,细胞体积缩小,核固缩,染色质浓缩聚集于核膜;流式细胞术检测显示G 1 峰前出现亚二倍体峰,转染48、72h后凋亡率分别为5.47%和5.63%( P nbsp; 0.05)。

We didn\'t find activated points in cerebellum and deeper brain.3.Acupuncture of sham point can significantly activated BA2,6,8,13,21,37,40,44,45,47,putamen and other areas.Both the left and right side of the brain have activated points,but points on the right side are more than those on the left.Both sides of middle temporal gyms,parietal lobule,supramarginal gyms and the lentiform nucleus have obvious activated points.The activated points mainly concentrated in the cerebral cortex,the deep-activated are mainly in the putamen.4. Sham needling in sham point can significantly activate BA6, 8,9,10,18,21,37,40,43,44.The activated points are mainly distributed in the right side of the brain.The left side also has some activated points;5.In the comparasion of Acupunture and sham-needling in S J5,we find that BA8 and cerebellum have distinct regional activated points;6.In the comparasion of acupuncture in SJ5 and sham point,we find BA2 and left cerebellar regions have activated points;7.Sham needling in S J5 compared with sham point,we find BA7,8,9,18 and other areas have activated points,the main activated points are at the left brain.It is not difficult to find that the distribution of activated points are mainly in the middle brain,no obvious activated points at the temporal lobe.

结果:通过对数据的处理和分析,我们初步发现:1、外关穴真针刺能显著激活Brodmann area45、37、44、40、22、13、37、47区、海马、杏仁核、黑质等区域,小脑左侧更明显,左侧颞叶皮层激活点多于右侧,且脑部左侧深层激活点多于右侧;2、外关穴假针刺能显著激活BA46、44、41、13、40、37、19区等区域,激活点主要集中表现在大脑皮层,以颞叶为主,小脑及深部未发现明显激活点;3、非穴真针刺组结果分析初步表明,BA2、6、8、13、21、37、40、44、45、47区以及壳核等区域有激活点,大脑左、右侧均有激活点,但右侧更多,双侧颞中回、顶叶下小叶、缘上回及豆状核有明显激活点,激活点主要集中在大脑皮层,深部激活点主要在壳核;4、非穴假针刺能显著激活BA6、8、9、10、18、21、37、40、43、44区等区域有激活点,主要分布在大脑的右侧,左侧也有不少激活点,就其具体分布主要在颞叶和额叶,少部分分布在顶叶和枕叶;5、外关穴真针刺与假针刺对比发现,BA8区和小脑等区域有明显的激活点;6、外关穴真针刺与非穴真针刺对比发现BA2区、左侧小脑等区域有激活点;7、外关穴假针刺与非穴假针刺对比发现,BA7、8、9、18等区有激活点,主要反应点在左侧大脑的枕叶中回、楔叶,顶叶楔前叶及额上回、中回,就其分布不难看出主要在大脑中部,颞叶未见明显激活点。

Donor cells in manipulation medium contain of 7% ethanol prior to injection were injected directly to enucleated cytoplasts.

供核细胞注核前置于含7%乙醇的操作液中,然后直接注入到去核的卵母细胞质内。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。