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To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中,苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变,并筛选在自生生长时期、共生生长时期的表型,以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因,我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法,发现lsrA基因主要在根瘤侵染区开始表达,表达时序也在侵染阶段左右,但晚于bacA基因表达;LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体,nifA和fixK基因的转录水平降低,nifH基因的转录被完全阻断,因此LsrA蛋白为根瘤发育所必需,是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因,并研究了它的调节特性。oxyR突变后,苜蓿中华根瘤菌对过氧化氢敏感性提高,适应性降低。

The result shows that: The variation rhythm of the sensitivity index changed unanimously for Jensen,Blank and Stewart water production functions of spring wheat in Liangzhou city,the volume is small in the former stage,then become large at the middle stage,and less at the last stage. Jensen and Stewart water production functions are better of spring wheat in Gulang County in 1987-1989. The Variation rhythm of water deficiency sensitive index is unanimous: the volume are small at first,then large at middle,and diminish at last. The Variation rhythm of water deficiency sensitive index is unanimous in Jensen,Blank and Minhas of flax in Liangzhou county in 1987-1989. The water deficiency sensitive index in Gulang district in 1987-1989 models bad,are too many minus.

结果表明:1985-1986 年凉州春小麦Jensen 模型、Blank 模型和Stewart 模型的敏感指数变化规律接近一致,在前期较小,中期增大,最后再减小。1987-1989 年古浪春小麦水分生产函数以Jensen 模型和Stewart 模型拟合较好,其敏感指数变化规律比较一致,敏感指标值前期较小,中期增大,后期又减小。1983-1985 年凉州亚麻水分生产函数以Jensen 模型、Blank 模型和Minhas 模型敏感指标值变化规律比较一致:先减小,后增大,最后再减小。1987-1989 古浪亚麻敏感指数出现负值较多,模型拟合较差。

The result shows that: The variation rhythm of the sensitivity index changed unanimously for Jensen,Blank and Stewart water production functions of spring wheat in Liangzhou city,the volume is small in the former stage,then become large at the middle stage,and less at the last stage. Jensen and Stewart water production functions are better of spring wheat in Gulang County in 1987-1989. The Variation rhythm of water deficiency sensitive index is unanimous: the volume are small at first,then large at middle,and diminish at last. The Variation rhythm of water deficiency sensitive index is unanimous in Jensen,Blank and Minhas of flax in Liangzhou county in 1987-1989. The water deficiency sensitive index in Gulang district in 1987-1989 models bad,are too many minus. 3 Establishing the non-linear model to definitive the irrigation quota and dimension of single crop,according the three prototype Precipitation,allocating the limited water optimally according to full water supply,95 percent,75 percent and 50 percent. Economy benefit is the biggest aim function,so getting three groups for irrigation quota,irrigation dimension and benefit,the biggest benefit is the most optimal result.

结果表明:1985-1986 年凉州春小麦Jensen 模型、Bl(来源:10a1ABC论文网www.abclunwen.com)ank 模型和Stewart 模型的敏感指数变化规律接近一致,在前期较小,中期增大,最后再减小。1987-1989 年古浪春小麦水分生产函数以Jensen 模型和Stewart 模型拟合较好,其敏感指数变化规律比较一致,敏感指标值前期较小,中期增大,后期又减小。1983-1985 年凉州亚麻水分生产函数以Jensen 模型、Blank 模型和Minhas 模型敏感指标值变化规律比较一致:先减小,后增大,最后再减小。1987-1989 古浪亚麻敏感指数出现负值较多,模型拟合较差。3 建立单作物种植条件下灌溉定额与灌溉面积的确定的非线性规划模型,将有限的水量按照充分供水,95%、75%和50%供水来分配水资源,以经济效益最大为目标函数,得出三组灌区灌溉面积和效益结果,其中以效益最大为最优解。

The results showed that(1) TES accelerated the meiosis and maturity of germ cells in leptotene, zygotene, pachytene, diplotene and diakinesis of prophase 1 of spermatocytes while STI inhibited the meiosis and maturity,(2) there was a significant e...

结果表明:(1)TES对前期Ⅰ5个分期的生殖细胞具有促进细胞分裂和成熟的作用,STI抑制细胞分裂和成熟;(2)TES和STI对前期Ⅰ粗线期药物效应显著;(3)TES和STI在小鼠体内最大有效效应分别持续约计70和50小时。

Preceding planning in real estate industry is paid little attention to and is directed without a set of systematic method, which directly leads to great resources waste, such as a large amount of vacant merchandise houses, new developed products continuing to be divorced from market.

随着房地产业的迅猛发展,房地产业正逐步成为国民经济新的增长点,但房地产前期策划水平仍处于初级阶段,开发企业不重视房地产前期策划,有些重视但没有一套系统的方法来指导,这直接导致大量商品房空置,而且下一轮开发的产品继续与市场脱节,造成了资源严重浪费。

Objective To determine the expression levels of vascular endotheliul growth factor and placental growth factor in the placeta tissue of patients with preeclampsia and eclampsia and explore the relationship of VEGF and PLGF to preeclampsia and eclampsia.

目的 检测血管内皮生长因子和胎盘生长因子在子痫前期及子痫患者胎盘组织中的表达,探讨两者与子痫前期和子痫的相关性。

Objective To analyze the clinical characteristics of end target organ damage of severe preeclampsia and the relationship of different patterns of end organ damage and perinatal outcomes.

目的探讨重度子癎前期(severe preeclampsia,S-PE)终末靶器官受累特点,分析早发型和晚发型重度子癎前期各种终末器官受累类型与围产结局的关系。

The Research of the Expression and the Relationship between Insulin Like Growth Factor Ⅱ and Matrix Metalloproteinase 9 in Pre-eclampsia.

胰岛素样生长因子Ⅱ及基质金属蛋白酶9在子痫前期中的表达及其相子痫前期,I

The molality of malondialdehyde in rind and pulp increased in early period and decreased later, but the time of peak appearing and range of increasing in early period were different in varieties: the peak appearing was earlier and range of increasing was larger in inbreed than those in hybrid and four season pummelo.

果皮、果肉中的丙二醛质量摩尔浓度前期上升,后期下降,但峰值出现的时间和前期增幅都不同,自交琯溪蜜柚的峰值来得早,增幅也大。

The activity of catalase in rind of both hybrid and inbreed showed the trend of decreasing, but increased in early period and decreased later in four season pummelo, while in inbreed pummelo pulp it showed the trend of decreasing, but increased in early period and decreased later in hybrid and four season pummelo.

自交和杂交琯溪蜜柚果皮中的过氧化氢酶活性呈下降的趋势,四季柚前期上升,后期下降;而果肉中的CAT活性变化是自交琯溪蜜柚呈下降趋势,杂交琯溪蜜柚和四季柚前期上升,后期下降。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

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