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Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池,将lipid A包被于非衍生样品池,分别建立以PGN、CpG DNA及lipid A为靶点的技术平台,对114种抗炎中药水提物进行筛选、评价其活性物质含量,并评估出针对上述三种病原分子均具有较高结合活性的中药;(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术,从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分;在体外实验中,测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力;MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响;ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用;在体内实验中,采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌,建立细菌脓毒症小鼠模型,观察活性组分对脓毒症模型小鼠的保护作用。

This idealised front/rear weight distribution, together with the frame's strength/rigidity balance and caster and trail dimensions make for a natural handling feeling that perfectly complements the machine's sensual and exciting power characteristics.

这idealised前/后座的重量分布,连同框架的强度/刚性的平衡和连铸机和径尺寸,使一个自然处理的感觉,能够完美地契合机器的感官刺激,功率特性。

In the previous researches, it was treated only as a tool to generate strings. It's biases during string generation was ignored. These biases include: The strings were ill-proportioned according to path, what the learning strings expressed was not what the testing strings tested, and the learning strings in each path couldn't express the rules of this path. So the string set of previous researches didn't express the grammars designed by experimenters completely, accurately and exclusively.

在以往研究中,研究者仅仅将人工语法作为现成的实验材料,以获取自己所需的刺激字符串为最终目的,忽略了生成字符串过程中可能出现的种种偏差,造成实验用字符串分布不均,测验字符串与学习字符串不对应,各线路学习字符串样例亦未能完整表达规则,因而生成的字符串集已经无法完整、准确、唯一地体现人工语法。

The results show that activation nutrition St-12 could activate the beneficial bacteria preferably, and restrain the harmful bacteria including sulfate reducing bacteria, iron bacteria and sulfer bacteria for oil recovery to a certain degree.

结果表明,激活剂配方St-12能较好地刺激有益内源菌群落的生长,且对硫酸盐还原菌、铁细菌及硫细菌等对采油不利的细菌起到了一定的抑制作用。

However, the finding that conditioned culture is also effective in stimulating wound healing may have important implications for the cure of the ischaemic complications of diabetes.

然而,有条件的环境也能有效地刺激伤口愈合,这个发现也许对治愈糖尿病缺血性并发症有重要的意义。

Car lovers favorite - Karting field near completion, the area of 9300 square meters, you can enjoy here to stimulate feelings of the coaster.

汽车爱好者的最爱--卡丁车场即将建成,面积有9300平方米,您在这里可以尽情地感受飞车的刺激。

Toyota stimulates personal and professional growth, shares opportunities for development and maximises individual and team performance.

丰田刺激个人和专业成长,共享的发展机遇,最大限度地提高个人和团队的表现。

In addition, VEGF is a selective mitogen for endothelial cells, and may directly stimulate cell proliferation.

实验发现,VEGF通过与其受体作用既可以提高血管通透性,有利于血浆蛋白外渗,又可有选择地直接作用于血管内皮细胞,刺激其分裂增殖。

AIM: To study the changes of mitochondrial membrane potential in mouse thymocytes stimulated by dexamethasone.

目的:研究地塞米松刺激小鼠胸腺细胞过程中线粒体膜电势的变化。

"I would presume that it has come to their attention that in order for the stimulus plan to work, we're going to have be successful selling our debt, and China is key to that," said Benn Steil, director of international economics at the Council on Foreign Relations in New York.

纽约外交关系协会国际经济部负责人斯戴尔说,我会这样猜想:他们已经意识到,为了让刺激计划奏效,我们就必须成功地出售债务,而中国是其中的关键;我们将指望他们能买走约30%的美国国债。

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