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刺激-反应

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Median nerve or deep peroneal nerve stimulation could inhibit completely or partially the deflection of ischemic ECG ST segment due to stimulation of dorsomedial hypothalamic nucleus.

电刺激正中神经和腓深神经均能抑制或部分抑制下丘脑背内侧核诱发的缺血性心电ST段偏移,以刺激MN更为明显。

Objective: To observe the changes of P-VEP of reverse occlusion and reverse occlusion combined with pattern stimulus therapy in esotropic kittens, and evaluate the reliability of amblyopic curative effect of pattern stimulus.

中文摘要:目的:观察斜视性弱视幼猫反转遮盖和反转遮盖+模式刺激治疗的图形视诱发电位(pattern visual evoked potential, P-VEP)改变,评价模式刺激治疗斜视性弱视幼猫效果的可靠性。

Objective: To observe the changes of P-VEP of reverse occlusion and reverse occlusion combined with pattern stimulus therapy in esotropic kittens, and evaluate the reliability of amblyopic curative effect of pattern stimulus.

目的:观察斜视性弱视幼猫反转遮盖和反转遮盖+模式刺激治疗的图形视诱发电位(pattern visual evoked potential, P-VEP)改变,评价模式刺激治疗斜视性弱视幼猫效果的可靠性。

The lateral rectus muscle of one eye was sectioned under anaesthesia in order to induce an esotropic squint in strabismic group and therapeutic group. In therapeutic groups, the non-squint eyelids of six 8-week-old esotropic kittens were sutured to induce the model of occlusion therapy, another six kittens were reversely occluded combined with pattern stimulus therapy.

斜视组和治疗组均在4周龄时行外直肌切断+部分切除手术,产生内斜视。8周龄时,治疗组行非斜视眼眼睑缝合造成遮盖治疗模型,其中6只猫只接受自然刺激治疗,另6只猫追加模式刺激治疗。

Regardless of the experiment conditions are under single stimulus or double stimulus, the result will come up with their apparent selective bias, when Ns meet with negative words, when Ss meet with positive words, when Is meet with introversion words and when Es meet with extroversive words. And our study proves that there exist the trait-congruency effects.

无论是单一刺激条件还是双重刺激条件下,Ns对负性词、Ss对正性词、Is对内向词以及Es对外向词都表现出明显的选择性偏向,研究证实了特质一致性效应的存在。

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞,建立小鼠T淋巴细胞活化、增殖的模型,以不同浓度的CPT作用于该模型,流式细胞术检测T细胞早期活化标志CD69分子的表达;以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数;以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色,建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型,通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用;采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用;利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

In fundic strips, ghrelin and GHRP-6 could decrease the on-response induced relaxation and increase off-response induced contraction of the muscle, with the effect of Ghrelin obviously stronger than that of GHRP-6.L-NNA could increase the effects of Ghrelin and GHRP-6-induced muscle contraction, and L-AA could decrease their effects.

在胃底部,Ghrelin和GHRP-6可使开电刺激诱导的平滑肌舒张活动减弱,断电刺激诱导的肌条收缩活动增强,且Ghrelin作用明显强于GHRP-6.L-NNA可显著增强Ghrelin和GHRP-6的促平滑肌收缩效应,但L-AA可显著减弱该作用。

Mean fungistatic rate to mold was99.99%.The acute oral LD50 was 1302.5 mg/kg in mice.This product was non-irritant to skin,but slightly irritant to eye.

结果:驱蚊保护时间达6h以上;抑菌率达99.99%;小鼠急性经口LD50为1302.25mg/kg;对家兔皮肤刺激积分值为0,48h后对眼刺激平均指数为0.5。

However,development of new device is needed and controlled clinical studies are required to further prove clinical efficacy of GES for obesity.

然而,为进一步证实胃电刺激的临床有效性,需开发新一代的胃电刺激器和进行临床对照试验。

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