划线培养

Furthermore, several bacteria genera are anaerobic or facultative anaerobic, and therefore cannot form pure cultures by ordinary streak plate method. Combination of well developed classical anaerobic microbiological techniques with modern molecular techniques, along with dynamic understanding of growth status and mode of odor production by different bacteria genera is critical for successful future studies.

此外， 猪粪尿排泄物中很多微生物是厌氧或兼性厌氧菌，无法通过普通的平板划线法而获得纯培养，今后应将传统的微生物厌氧培养方法与现代微生物分子生物学技术结合，动态了解和掌握不同菌群的生长状态及在恶臭产生过程中的作用。

Methods: The marine bacteria were isolated with the infinite dilution and plate streak technique and screened for antimitosis active strains by Pyricularia Oryzae model. The active strain 32-11-2-2 was subjected to morphological, physiological and biochemical study, and was classified by the analysis of the 16S rDNA sequence. Results A total of 421 strains of marine bacteria were isolated from marine water and sediment samples; 54 of them had antimitotic activity.

采用无限稀释和平板划线法分离微生物样品，利用稻瘟霉模型进行活性筛选；并对其中的活性菌株32-11-2-2的形态、培养特征以及生理生化特征进行研究，使用16S rDNA序列分析的方法对菌株32-11-2-2进行了分类鉴定。

Methods Porcine coronary SMCs were incubated with serum-free medium containing different concentrations of simvastatin. Infiltration of 3H-TdR into SMC DNA was measured as an index of cell proliferation. Simvastatin's effect on migration was tested with Sarkar's technique.

采用体外猪冠状动脉SMC培养技术，以3H-TdR的参入量表示冠状动脉的SMC DNA合成情况，应用划线方法测定冠状动脉SMC的迁移距离，观察辛伐他汀对OX-LDL诱导的冠状动脉SMC增殖和迁移的影响。

Methods The 3-5 generation fibroblasts were inoculated in 24-well with rat-tail collagen. A denuded area was made when the 3 generation of the fibroblasts was influent into a monolayer. Cells were then exposed to the presence of various concentrations of viper venom, mitomycin C, and the cells migrated into the denuded area were counted evely 6 hours.

将培养的第3~5代人结膜下成纤维细胞接种于涂有鼠尾胶原的24孔板中，待细胞生长融合后在培养板上划线，造成无细胞的裸露区，分别加人不同浓度的蛇毒制剂、丝裂霉素C及二者的混合液，每6h观察记录移行至裸露区的细胞。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。