划线培养

Furthermore, several bacteria genera are anaerobic or facultative anaerobic, and therefore cannot form pure cultures by ordinary streak plate method. Combination of well developed classical anaerobic microbiological techniques with modern molecular techniques, along with dynamic understanding of growth status and mode of odor production by different bacteria genera is critical for successful future studies.

此外， 猪粪尿排泄物中很多微生物是厌氧或兼性厌氧菌，无法通过普通的平板划线法而获得纯培养，今后应将传统的微生物厌氧培养方法与现代微生物分子生物学技术结合，动态了解和掌握不同菌群的生长状态及在恶臭产生过程中的作用。

Methods: The marine bacteria were isolated with the infinite dilution and plate streak technique and screened for antimitosis active strains by Pyricularia Oryzae model. The active strain 32-11-2-2 was subjected to morphological, physiological and biochemical study, and was classified by the analysis of the 16S rDNA sequence. Results A total of 421 strains of marine bacteria were isolated from marine water and sediment samples; 54 of them had antimitotic activity.

采用无限稀释和平板划线法分离微生物样品，利用稻瘟霉模型进行活性筛选；并对其中的活性菌株32-11-2-2的形态、培养特征以及生理生化特征进行研究，使用16S rDNA序列分析的方法对菌株32-11-2-2进行了分类鉴定。

Methods Porcine coronary SMCs were incubated with serum-free medium containing different concentrations of simvastatin. Infiltration of 3H-TdR into SMC DNA was measured as an index of cell proliferation. Simvastatin's effect on migration was tested with Sarkar's technique.

采用体外猪冠状动脉SMC培养技术，以3H-TdR的参入量表示冠状动脉的SMC DNA合成情况，应用划线方法测定冠状动脉SMC的迁移距离，观察辛伐他汀对OX-LDL诱导的冠状动脉SMC增殖和迁移的影响。

Methods The 3-5 generation fibroblasts were inoculated in 24-well with rat-tail collagen. A denuded area was made when the 3 generation of the fibroblasts was influent into a monolayer. Cells were then exposed to the presence of various concentrations of viper venom, mitomycin C, and the cells migrated into the denuded area were counted evely 6 hours.

将培养的第3~5代人结膜下成纤维细胞接种于涂有鼠尾胶原的24孔板中，待细胞生长融合后在培养板上划线，造成无细胞的裸露区，分别加人不同浓度的蛇毒制剂、丝裂霉素C及二者的混合液，每6h观察记录移行至裸露区的细胞。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。