切短的

Renqiu existence machining Manufacturing Co., the main production Nail Die, drawing module, Nail knife, cut materials and various special-shaped knife alloy die, as long as there are samples, drawings or my company can in the shortest possible time to produce Customer satisfaction with the mold.

任丘永固机械加工制造有限公司，主要生产制钉模，拉丝模，制钉刀，切料刀及各种异型合金模具，客户只要有样品或图纸我公司便可在最短的时间内生产出客户满意的模具。

Objective To compare the efficiency of constructing three truncated Echinococcus multilocularis18 gene expression vector using two clone methods.

目的比较3种截短的泡球蚴Em18基因聚合酶链反应扩增产物经TA克隆和直接酶切2种方法，在原核表达质粒构建中的运用。

The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物，采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框，将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达，通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化，采用Western-blot检测其IgE结合活性。

The Chitinase of Saccharomyces cerevisiae(CTS1-2) is an Endochitinase which is endogenous in Saccharomyces cerevisiae. Its recognition and slipt site lies in the β-1,4 glucosidic linkage of homopolymer. Included in the amino acid sequence from N-terminal to C-terminal of the Saccharomyces cerevisiae chitinase are signal peptide sequence, catalytic domain, binding domain and a C-terminal shorten region.

酿酒酵母几丁质酶(Chitinase，CTS1-2)是一种来自酿酒酵母的内切几丁质酶，它的酶切位点位于其同聚物内的β-1，4连接的糖苷键，酶蛋白分子结构从N端到C端依次为信号肽、几丁质催化区、几丁质结合区以及一个短的C末端区。

Methods:The whole mature protein coding sequence of three truncated dystrophin gene was amplified by RT-PCR method applied to human muscle cDNA. The fragment was inserted into prokaryotic expression vector pET28a plasmid.

以人肌肉组织cDNA为模板，采用RT-PCR扩增三个截短的肌营养不良蛋白cDNA，分别克隆入原核表达载体pET28a中，经限制性内切酶双酶切及DNA序列分析鉴定目的基因。

Stipules green, falcate, rarely ovate or ovate-lanceolate, herbaceous, margin sharply serrate or lobed, rarely entire, apex acute or acuminate; petiole sparsely pilose or pubescent; leaf blade interrupted imparipinnate with (2 or)3 or 4 pairs of leaflets, reduced to 3 leaflets on upper leaves; leaflets sessile or shortly petiolulate, obovate, obovate-elliptic, or obovate-lanceolate, 1.5–5 × 1–2.5 cm, abaxially appressed pilose on veins, or densely pubescent or tomentose-pubescent between veins, rarely glabrescent, markedly or sparsely glandular punctate, adaxially pilose, or hirsute or hirtellous on veins, rarely glabrescent, base cuneate to broadly so, margin acutely to obtusely serrate, apex rounded to acute, rarely acuminate. Inflorescence terminal, spicate-racemose, branched or not; rachis pilose.

绿色，镰刀形的托叶，卵形或卵状披针形的很少，草本，边缘锐切成锯齿状或者有叶，很少全缘，先端锐尖或渐尖；叶柄疏生柔毛或短柔毛；叶片间断奇数羽状具(2或)3或4小叶，退化至3小叶在上部叶片上；短的小叶无柄或具小叶柄，倒卵形，倒卵状椭圆形，披针形或倒卵形， 1.5-5 * 1-2.5 厘米，背面的贴伏的具柔毛的在脉上，在脉之间的或密被短柔毛的或被绒毛的短柔毛，很少脱落无毛显著具点，正面具柔毛的或疏生腺体，或具粗毛或在脉上，很少脱落无毛，基部楔形到宽楔形，花序顶生，穗状总状，与否的分枝；轴具柔毛。

Since Watson and Crick have brought forward the model of DNA helix structure and explain about it's reproduce mechanism in molecule level, research work concerning DNA has been continuously lucubrated. However, whether translating inheritance information and rebuilding DNA molecule via menstruating DNA sequence, or to date the most compelling gene project, chromosome protract et. al, there all need a sort of molecule scissors cleaving DNA, DNA cleavage reagent. At present, tool enzyme of cleaving DNA is mostly DNA restriction enzymes founded in 70 years, which only cleave DNA at specific 4-8 base pair sequences that are usually palindromic, so this restricts the at very fast speed developing of gene technology at certain extent.

自从Watson和Crick提出DNA的双螺旋结构并对其复制机制在分子水平上进行解释后，使得对DNA的研究不断深入，但无论是通过测定DNA的序列来破译遗传信息、改造DNA分子，还是目前最令人关注的基因工程、染色体绘制等，都需要一种断裂DNA分子的&剪刀&——DNA断裂试剂，目前断裂DNA的工具酶主要是上个世纪70年代发现的DNA限制性内切酶，但所知的Ⅱ型限制性内切酶的DNA序列识别长度较短（约为4～8个碱基对），而且一般要求回文结构，这就给飞速发展的基因工程技术带来一定的限制。

Mobile positioning system or dynamic table so that the laser head is cutting material in the system-driven subcapital high speed, laser head from the light source and cutting heads, light source produces a very short wavelength focused laser beam, laser beam through cutting heads, vertically focused the material being cut on the surface, heating and melting, evaporation is cutting material, resulting in the closure of Lancing, slotted openings formation pads.

移动定位系统妪动工作台或激光头，使得被切割材料在系统驱动头下高速运动，激光头由光源部分和切割头组成，光源部分产生波长很短的聚焦激光束，激光束通过切割头，垂直聚焦在被切割的材料表面上，加热、融化、蒸发被切割材料，形成切缝，闭合的切缝形成焊盘开孔。

Their helmets and boots were off, and their MA5Ks, cut-down versions of the standard MA5B assault rifle, were close at hand.

他们脱下了头盔和靴子，他们的MA5K步枪好像切短的标准MA5B突击步枪，就放在手边。

The experiment contrasted the feed value between rice stalk silages and whole corn silage.

相同品种的水稻秸青贮，整株青贮与切短青贮之间存在差异。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。