切短物

The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物，采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框，将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达，通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化，采用Western-blot检测其IgE结合活性。

The Chitinase of Saccharomyces cerevisiae(CTS1-2) is an Endochitinase which is endogenous in Saccharomyces cerevisiae. Its recognition and slipt site lies in the β-1,4 glucosidic linkage of homopolymer. Included in the amino acid sequence from N-terminal to C-terminal of the Saccharomyces cerevisiae chitinase are signal peptide sequence, catalytic domain, binding domain and a C-terminal shorten region.

酿酒酵母几丁质酶(Chitinase，CTS1-2)是一种来自酿酒酵母的内切几丁质酶，它的酶切位点位于其同聚物内的β-1，4连接的糖苷键，酶蛋白分子结构从N端到C端依次为信号肽、几丁质催化区、几丁质结合区以及一个短的C末端区。

With ammonium persulfate/sodium thiosulfate as Redox initiator system,a binder based on vinyl acetate/acrylate copolymer used for chopped strand mat was prepared at low temperature.

以过硫酸铵/硫代硫酸钠为氧化还原引发体系，在低温下研究制备出了短切毡用的醋酸乙烯酯与丙烯酸酯类共聚物粘结剂。

In addition, ppg provides industrial coatings to various companies including haier, nokia, motorola, whirlpool and dell. ppg architectural coatings are widely used in many well-known structures including those in tian anmen square and the flag post, beijing oriental plaza, beijing international exhibition center, beijing international airport, the shanghai jinmao building, shanghai pudong international airport, shanghai oriental pearl tower, guangzhou baiyun international airport, tianjin jinhuang building and taida building.ppg's fiber glass business entered china's marketplace in the 1980s and established manufacturing sites in taiwan jiayi, jiangsu kunshan and shandong zibo. in march 2006, the world's largest fiber glass furnace, costing $120 million, was put to production in kunshan, boasting more than 70 thousands tons of output. in 2006, ppg invested jointly with sinoma in zibo to launch a fiber glass plant that will have more than 200,000 tons of annual output.

除涂料业务外， ppg的玻璃纤维也于80年代进入中国，并已先后在台湾嘉义、山东淄博和江苏昆山投资了生产基地；2006年3月，在昆山基地投资的1.2亿美元，年产7万吨的世界最大的玻纤丝窑正式投产；2006年与中材金晶合作在淄博投资建成了一个年产20万吨以上的玻纤生产基地；并计划在苏州与必成实业共同设立一个玻纤研发中心，以支持ppg在亚太区客户的应用问题。ppg玻纤在全球共拥有九座生产基地，并四座研发中心，以及遍布世界营销与技术服务网的全球玻纤领导者，制造连续拉丝玻璃纤维产品，包括短切纤维、直接纱、玻纤纱、无捻粗纱和玻纤卷物等。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。