切短

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

The transurethral partly resection of prostate is a good treatment for the BPH patients at high risk,it has the advantages of shorter operation time ,less bleading early resumption and satisfactory results , the prostatectomy is more trauma , but can totally cut the prostate is the foundamental operation of prostatectomies , it can be chosed if the TURP is failure and it is suitable to be developed in the matrical hospital and the beginners .

经尿道前列腺部分电切术治疗高危前列腺增生的效果理想，具有手术时间短、出血少、创面小、恢复快，并发症少等优点。耻骨上前列腺摘除术创伤较大，但切除腺体彻底，是一切前列腺手术的基础，可作为腔内手术失败后的备选治疗方案，适宜在广大基层医院及初学者间开展。

Additionally, because recovery times are short for procedures such as myringotomy and tubes, circumcision, and inguinal herniorrhaphy, early discharge from the facility is feasible.

恢复时间短的手术如鼓膜切开与置管术，包皮环切术和腹股沟疝修补术早期离开医院都是可行的。

Bioinformatics studies of the functional impact of alternative splicing have revealed a wide range of regulatory mechanisms, from NAGNAG sites that add a single amino acid; to short peptide segments that can play surprisingly complex roles in switching protein conformation and function (as in the Piccolo C2A domain); to events that entirely remove a specific protein interaction domain or membrane anchoring domain.

选择性剪切的功能性影响的生物信息学研究揭示了各式各样的调控机制，从添加一个单氨基酸的NAGNAG位点；到可以在开关蛋白质构象和功能(如在Piccolo C2A区域)中起到惊人地复杂作用的短肽片段；到完全移除一个特定蛋白质相互作用区域或膜锚定区域的事件。

Because studies of SVMP of Naja mocambique mocambique are more abundant, hence taking this as the standard for comparison between all these three SVMP, and using synthesized short peptides as the substrates for SVMP to test enzyme activity.

在实验中利用合成之短链？肽当作测试酵素活性的受质，发现三者切PSGL以及GPIb的位置一样。

Results Bleeding occurred in 18 cases postoperatively, with 5 cases in the retropubic prostatectomy group,12 in TURP group and 1 in TUVP group.

经尿道前列腺电切术以其创伤小、手术时间短、术后恢复快等优点，已是公认治疗前列腺增生的金标准[2-3]。

To meet the diverse needs of our customers, our company continuously developing new products and has introduced from abroad advanced Rewinder, Slitter, printing machine can be arbitrary client request processing, and welcome new and old customers come to discuss, we will be in the shortest time possible quality and efficient completion of orders, in order to provide the most satisfaction with your products.

为满足广大客户的各种需求，我公司不断开发新产品，并拥有从国外引进的先进复卷机、分切机、印花机可按客户要求任意加工，欢迎广大新老客户前来洽谈，我们将在最短的时间内优质高效地完成订货，为你们提供最为满意的产品。

To meet the diverse needs of our customers, our company continuously developing new products, and has advanced Rewinder, Slitter, printing machine can be arbitrary client request processing, welcome old and new customers came to negotiate, we will in the shortest possible time, quality and efficient completion of orders, in order to provide the most satisfaction with your products.

为满足广大客户的各种需求，我公司不断开发新产品，并拥有先进复卷机、分切机、印花机可按客户要求任意加工，欢迎广大新老客户前来洽谈，我们将在最短的时间内优质高效地完成订货，为你们提供最为满意的产品。

In this paper, the effect of silicane coupling agent γ-aminopropyl triethy-oxysilane(A-1100) on interface properties of short glass fibre reinforced polycarbonate is report-ed.

针对短切玻纤增强聚碳酸酯体系，研究了硅烷偶联剂γ-氨丙基三乙基硅烷(A-1100)在上述复合体系中的界面偶联特性。

Due to very short changing intervalls additional film slitting is required no more.

由於换卷时间极短，所以不再需要另外的薄膜分切操作。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。