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The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物,采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框,将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达,通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化,采用Western-blot检测其IgE结合活性。

Carbon fiber cement composite showing its excellent piezoelectricity, the application of its piezoelectricity was studied.

鉴于短切碳纤维和碳纤维毡水泥基复合材料具有很好的压敏性,对CFCC的压敏性进行了应用研究。

CFCC and CFFCC beam's Seebeck effect has shown good linear character,and Seebeck curve can nearly cover when temperature in increasing and decreasing process.

短切碳纤维混凝土涂层梁及碳纤维毡混凝土涂层梁的Seebeck效应均显示较好的线性,且升温阶段和降温阶段几乎重合。

With ammonium persulfate/sodium thiosulfate as Redox initiator system,a binder based on vinyl acetate/acrylate copolymer used for chopped strand mat was prepared at low temperature.

以过硫酸铵/硫代硫酸钠为氧化还原引发体系,在低温下研究制备出了短切毡用的醋酸乙烯酯与丙烯酸酯类共聚物粘结剂。

Our company produces all kinds of chopped strand mat for 6000Ton per year , 5000 Ton woven roving per year, 30 million square meters fiberglass mesh per year.

本公司年产各种规格短切毡6000余吨;玻纤方格布5000余吨;各种玻纤纱3000余吨;玻纤网格布2000万平方米。

The Company an annual output of various specifications chopped more than 5,000 tons of carpet; fiberglass rovings Plaid 3,000 tons; a variety of more than 3,000 tons of glass fiber yarn; Fiberglass Mesh 20 million square meters.

本公司年产各种规格短切毡 5000 余吨;玻纤无捻粗纱方格布 3000 余吨;各种玻纤纱 3000 余吨;玻纤网格布 2000 万平方米。

The sludge in SBMBR1 presented granular characteristics and settled very well. The sludge in SBMBR1was brown and the sludge flocs was smashed by the shear force due to high intensity of aeration. The sludge size decreased and the sludge flocs blended with water forming ropy solutions, and there was no distinguish interface between water and sludge. Low sludge age and high loading operation enabled the sludge in SBMBR1 keeping higher sludge activity and the MLVSS/MLSS ratio and SOUR values of SBMBR1 were higher than SBMBR2. Both in SBMBR1 and SBMBR2, there were large amount of zoogloea, bacillus, coryneform of bacteria, and pediococcus. However, in SBMBR1 there were also a lot of Protozoa like epistylis and etc. A certain amount of filamentous bacteria can also be found in SBMBR1. The biological species in SBMBR1 were much more abundant than that in SBMBR2.3. Membrane fouling on flat-sheets membrane was observed and analyzed with AFM, SEM and TEM. After the measurement of membrane surface EPS and membrane surface ETX analysis, the pollutants composition on the surface of flat-sheets membrane was calculated.

从外观观察SBMBR1反应器中的活性污泥呈浅黄色,随着运行时间的增加,污泥粒径逐步增加,SV、SVI值逐步下降,呈现出较强的颗粒特征,沉降性能良好;SBMBR2污泥呈土黄色,污泥絮体被高强度的曝气所产生的切应力所打碎,污泥粒径迅速降低,絮体较为细碎,和水相混合成均一粘稠的溶液,看不到泥水分界面;低泥龄、高负荷运行,使得SBMBR1可以保持较高的微生物活性,MLVSS/MLSS值、SOUR值均高于SBMBR2;两级反应器活性污泥中均有大量的菌胶团,长杆菌、短杆菌和球菌是构成主体,但SBMBR1活性污泥中含有大量固着型原生动物如累枝虫等,还存在着一定数量的丝状菌,生物相要比SBMBR2丰富。3、借助原子力显微镜、扫描电镜、透射电镜等手段对平板膜污染现象进行观测和分析,并通过膜表面污染物EPS测定、污染膜表面能谱分析,得出平板膜表面污染物的组成。

The most distinct intensification in maximum azimuthally mean tangential velocity occurs when the convective patch is positioned in RMW, the second in the interior region.

RMW附近扰动使最大平均切向风速增大最明显,其次是内核区,而且这种增强表现出显著的震荡特征,震荡周期约为6~7h,内核区的震荡较弱,震荡周期略短。

This specimen was one of the first RRSO cases in our experience and on review of the original report, this ovary was not dissected into multiple slices along its short axis but was only bivalved along its long axis.

此例是我们最开始处理RRSO这类病例中的一例。翻开这例的原始病理报告,我们发现其卵巢标本没有沿着短径切成多个薄薄的小段,而只是沿着长径一分为二。

Carberry says Boeing is confident that legal hurdles can be surmounted: fibers reclaimed from retired Boeing 777 parts and a host of military jets have been used to make a single, nonstructural part for a military aircraft.

碳纤维的等级决定了其价格,不过Carberry估计再生纤维在公开市场上的价格会游离在每磅5.5美元到6.00美元之间,约为原始短切纤维价格的三分之一到一半。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。