切的

Methods The target cDNA was amplified by PCR and PCR products were indigested by restriction enzyme Nco Ⅰ and Hind Ⅲ. Then the indigested products were recombined into pET-BBH and pET-BBH was expressed in E. coli BL21 (DE3) by IPTG inducing. Next, the expressed proteins were identified by SDS-PAGE, western blot and enzyme activity test. Finally, fluorescence quantitative PCR was used to test its expression quantity in different worm stages.

PCR扩增GAMMA-BBH cDNA基因，产物经NcoⅠ和HindⅢ限制性内切酶酶切后连接至原核表达载体重组为pET-BBH，在BL21(DE3)中用IPTG诱导表达，SDS-PAGE、Western blot和酶活性测定鉴定表达产物，用荧光定量PCR方法检测广州管圆线虫不同虫龄GAMMA-BBH的表达量。

The proximal jejunum of the graft was anastomoscd to that of recipient, and an jejunostomy was created by exteriorizing the distal end of the donor jejunum with the recipient distal jejunum anastomosed to the side of the graft blow the stoma.

肠道重建采用切除受体部分回肠，将供肠近端与受体肠近切端端端吻合，供肠远端与受体肠远切端端侧吻合后把供肠远端拖出，腹壁造口的方法。

Chelsea were a goal down after 19 minutes through Lubomir Michalik but Salomon Kalou and an own goal by Jussi Jaaskelainen put them in front by the interval.

切尔西比赛第19分钟，对方后卫梅查理克先攻入一球，但是卡劳和雅斯克莱宁的乌龙球帮助切尔西在半场结束时取得领先。

Kala then explained how negotiations had gone with Chelsea:'Initially, I said I wanted to go to Chelsea as I hadn't played that much.

卡哈接着解释了和切尔西谈判破裂的一些问题：一开始，我说自己想转会切尔西，因为我不能获得很多出场机会。

Restriction sites polymorphisms of rRNA gene for Leptospira can be used to classify and identify Leptospira in China and also to study on molecular epidemiology for leptospirosis.

rRNA基因限制性内切酶酶切位点多态性可用于钩体分类，而且是钩体病分子流行病学调查的一种好方法。

Compared with the monopolar electrode system, bipolar electrode system was associated with a reduction in operation time and absorption volume of uterine distention medium and makes less influence on blood biochemistry.

与单极电切术比较，双极电切术手术时间短，膨宫液吸收量少，对患者生化的影响更小。

Chelsea Football Club is happy to confirm that Petr Cech has returned home from hospital.

切尔西俱乐部高兴的宣布，门将彼得·切赫已经出院回到了家中。

Petr Cech reveals on Chelsea TV tonight that he is targeting a mid-January return to full training.

切赫在今晚的切尔西电视节目中透露他争取在1月中旬恢复完整训练。

The Charon library DNA was then digested with restriction enzymes BamH Ⅰ and Hind Ⅲ, and DNA fragments of length of about 0.5-1. 5kb was subcloned into plasmid pUC 18 which was also digested by BamH Ⅰ and Hind Ⅲ and dephosphorylated by alkaline phosphatese before being ligated with Charon fragments.

抽提λCharon文库总DNA，用BamHⅠ和HindⅢ完全酶切电泳，用低熔点琼脂糖回收长约0.5-1.5kb的DNA片段；连接到pUC18上，pUC DNA先用BamHⅠ和HindⅢ酶切，后用碱性磷酸酯酶脱磷。

We own series of advanced Japanese KOMORI 4-color Lithrone 40 and Lithrone S40, Heidelberg 4-color offset press, Automatic paper-cutter, high-speed cardboard box sticking machine, and a set of photomechanical equipment etc.

有国际先进的日本小森KOMORI LITHRONE 40及日本小森KOMORI LITHRONE S40对开机，海德堡SPEED MASTER对开四色胶印机，全自动波拉切纸机，自动糊盒机，自动模切机等。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。