切的

Methods: Two sets of primers were designed according to the cyclostyle of pcDNA3.1-HBV-YVDD, and the full-length of HBV genome with different enzyme digestin spot was amplified.

以pcDNA3.1-HBV-YVDD为模板设计两对引物，扩增带不同酶切位点的HBV全基因，扩增产物纯化后，经酶切、连接，得到pcDNA3.1-HBV-YVDD重组体。

From April 2000 to June 2002, 512 cases of laparoscopical urologic operation have been performed through transperitoneal and retroperitoneal or extraperitoneal approach: 41 cases of nephrectomy, 36 cases of radical nephrectomy, 10 cases of nephroureterectomy with cystoscopic en bloc excision of the distal ureter and bladder cuff, 3 cases of partial nephrectomy, 78 cases of partial adrenalectomy or resection of adenomas, 71 cases of total adrenalectomy, 17 cases of ureterolithotomy, 122 cases of decortication of renal cyst, 1 case of Anderson-Hynes dismembered pyeloplasty, 7 cases of renal pedical lymphatics disconnection, 2 cases of relative living donor nephrectomy.

本课题通过对223例肾疾病、149例肾上腺疾病、17例输尿管结石病人进行腹腔镜及后腹腔镜手术，其中肾囊肿去顶术140例、单纯肾切除术41例、根治性肾切除术36例、肾输尿管全切及输尿管口膀胱袖套状切除术10例、后腹腔镜肾部分切除术3例、Anderson-Hynes肾盂成形术1例、肾蒂淋巴管离断术7例、腹腔镜和后腹腔镜活体亲属供肾切取术各1例、肾上腺部分或单纯肿瘤切除术78例、肾上腺全部切除术71例、输尿管切开取石术17例。记录手术时间、手术中失血量、肠道功能恢复时间及并发症，将结果与传统开放手术和国外学者的腹腔镜手术结果进行比较，对数据进行统计分析。

The analysis of the results show as follow:(1)The consolidated-undrained creep, which doesn't include the volume strain, can be expressed by logarithmic functions as a type of under a different deviator stress.(2)The shear creep behaves in a linear elastic character in a lower stress level, and it is a nonlinear behavior in a higher stress level.

分析表明：(1)固结不排水蠕变中没有体积变化，不同偏应力下剪切蠕变可用对数形式表达；(2)固结不排水剪切蠕变在低应力水平下表现为线性粘弹性，而高应力水平时则具非线性的特性。

Stud shear reinforcement can be arranged in perpendicular and diametrical directions, and it should be evenly arranged around the column region.

抗冲切锚栓的布置方式包括正交布置和径向布置，且抗冲切锚栓应在柱周边区域均匀分布。

The carving is done in the kitchen, and not on the dining table as in the West.

格格切肉是在厨房里进行的，而不是像西方那样在餐桌上切肉。

Methods: A retrospective study on the data of 63 cases with CIN treated with hysteroscopic electrosurgery was done at our hospital between January 2004 and June 2007. The clinical-pathological date were analyzed.

回顾性分析2004年1月～2007年6月在我院接受宫腔镜宫颈锥切术63例CIN患者的临床病理资料，记录手术时间、手术出血量，分析手术前、后病理符合情况，术后切缘阳性及再次手术情况。

Use this shredding mechanism, the length of the stem is 30 mm theoretically, it has met the ensilage requirements.

使用此切碎装置，玉米茎杆切碎理论长度 30 mm，达到了青贮的要求。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

RESULTS:①Enzymolysis and nucleotide sequence analysis of recombinant plasmids showed that two VEGF-C siRNA eukaryotic expression vectors were constructed successfully.

结果：①重组质粒的酶切、核苷酸序列分析结果：经酶切和测序鉴定证实成功构建了血管内皮生长因子C小干扰RNA表达载体，建立了稳定转染细胞株。

In exterior differentiation, one studies tensors, exterior algebra, tangent bundle, tangent vector fields, one-parameter groups action on a manifold, Frobenius's theorem, tensors fields, exterior differential form, integration on manifolds, Stokes's theorem etc.

外微分部分主要涉及外代数、切丛，光滑切向量场，单参数变换群，光滑张量场，外微分形式，Frobenius定理，外微分形式的积分，Stokes定理及其应用等。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。